Antibiotic Susceptibility of Staphylococcus aureus Plankton and Biofilm Forms Isolated in Implant-Associated Infection

Babushkina, I. V.; Mamonova, I. A.; Ulyanov, Vladimir Yu.; Гладкова, Е. В.; Shpinyak, S. P.

doi:10.1007/s10517-021-05328-8

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…There was strong consensus (79% agreement) with the “No” recommendation . Laboratory studies support that biofilm maturity increases generally with age for cultures up to 3 weeks 10 . The degree of tolerance is dependent on the antimicrobial agent, the species, the treatment exposure time and the model system.…”

Section: Resultsmentioning

confidence: 96%

“…Laboratory studies support that biofilm maturity increases generally with age for cultures up to 3 weeks. 10 The degree of tolerance is dependent on the antimicrobial agent, the species, the treatment exposure time and the model system. Thus, since the degree of tolerance depends not only on the age of the biofilm but many other factors, 11 it was recommended that a single measurement is not sufficient to determine whether a biofilm is mature.…”

Section: Conceptual: Understanding Concepts In Bacterial Tolerance An...mentioning

confidence: 99%

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The 2023 Orthopedic Research Society's international consensus meeting on musculoskeletal infection: Summary from the in vitro section

Hickok,

Li,

Oral

et al. 2024

Journal Orthopaedic Research

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Antimicrobial strategies for musculoskeletal infections are typically first developed with in vitro models. The In Vitro Section of the 2023 Orthopaedic Research Society Musculoskeletal Infection (ORS MSKI) international consensus meeting (ICM) probed our state of knowledge of in vitro systems with respect to bacteria and biofilm phenotype, standards, in vitro activity, and the ability to predict in vivo efficacy. A subset of ICM delegates performed systematic reviews on 15 questions and made recommendations and assessment of the level of evidence that were then voted on by 72 ICM delegates. Here we report recommendations and rationale from the reviews and the results of the internet vote. Only 2 questions received a >90% consensus vote, emphasizing the disparate approaches and lack of established consensus for in vitro modeling and interpretation of results. Comments on knowledge gaps and the need for further research on these critical MSKI questions are included.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 96%

Section: Conceptual: Understanding Concepts In Bacterial Tolerance An...mentioning

confidence: 99%

The 2023 Orthopedic Research Society's international consensus meeting on musculoskeletal infection: Summary from the in vitro section

Hickok,

Li,

Oral

et al. 2024

Journal Orthopaedic Research

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“…This contrasts with the bactericidal properties of other surfaces such as dragon y and cicada wings which demonstrate the greatest potency against gram-negative bacteria [23]. An anti-biofouling surface that prevents gram-positive bacteria from attaching and forming bio lms would play an important role in preventing infection, since antibiotics are much more effective against planktonic cells than against bio lms [24,25].…”

Section: Discussionmentioning

confidence: 98%

Carbon-Infiltrated Carbon Nanotubes Inhibit the Development of Staphylococcus aureus Biofilms

Bowden,

Wells,

Miller

et al. 2023

Preprint

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Background: Staphylococcus aureus forms biofilms that cause considerable morbidity and mortality in patients who receive implanted devices such as prosthetics or fixator pins. An ideal surface for such medical devices would inhibit biofilm growth. Recently, it was reported that surface modification of stainless steel materials with carbon-infiltrated carbon nanotubes (CICNT) inhibits the growth of S. aureus biofilms. The purpose of this study was to investigate this antimicrobial effect on titanium materials with CICNT coated surfaces in a variety of surface morphologies and across a broader spectrum of S. aureus isolates. Results: Study samples of CICNT-coated titanium, and control samples of bare titanium, a common implant material, were exposed to S. aureus. Viable bacteria were removed from adhered biofilms and quantified as colony forming units. Scanning electron microscopy was used to qualitatively analyze biofilms both before and after removal of cells. The CICNT surface was found to have significantly fewer adherent bacteria than bare titanium control surfaces, both via colony forming unit and microscopy analyses. This effect was most pronounced on CICNT surfaces with an average nanotube diameter of 150 nm, showing a 2.5-fold reduction in adherent bacteria. Since S. aureus forms different biofilm structures by isolate and by growth conditions, we tested 7 total isolates and found a significant reduction in the biofilm load in six out of seven S. aureus isolates tested. To examine whether the anti-biofilm effect was due to the structure of the nanotubes, we generated an unstructured carbon surface. Significantly more bacteria adhered to a nonstructured carbon surface than to the CICNT surface, suggesting that the topography of the nanotube structure itself has anti-biofilm properties. Conclusions: The CICNT surface possesses antimicrobial properties that result in fewer adherent S. aureus bacteria. These antimicrobial properties are consistent across multiple isolates of S. aureus and are affected by nanotube diameter. The experiments performed in this study suggest that this effect is due to the nanostructure of the CICNT surface.

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“…To screen ArlSHK ATPase activity inhibitors, the Promega Kinase-Glo(R) Luminescent Kinase Assay was used as previously described [ 44 , 71 , 72 , 73 ]. The optimized condition for reactions was as follows: 2 μg purified ArlSHK, 3.5 μM ATP, 30 min incubation at room temperature.…”

Section: Methodsmentioning

confidence: 99%

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

Wang,

Bai

et al. 2024

Microorganisms

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Staphylococcus aureus can form biofilms on biotic surfaces or implanted materials, leading to biofilm-associated diseases in humans and animals that are refractory to conventional antibiotic treatment. Recent studies indicate that the unique ArlRS regulatory system in S. aureus is a promising target for screening inhibitors that may eradicate formed biofilms, retard virulence and break antimicrobial resistance. In this study, by screening in the library of FDA-approved drugs, tilmicosin was found to inhibit ArlS histidine kinase activity (IC50 = 1.09 μM). By constructing a promoter-fluorescence reporter system, we found that tilmicosin at a concentration of 0.75 μM or 1.5 μM displayed strong inhibition on the expression of the ArlRS regulon genes spx and mgrA in the S. aureus USA300 strain. Microplate assay and confocal laser scanning microscopy showed that tilmicosin at a sub-minimal inhibitory concentration (MIC) had a potent inhibitory effect on biofilms formed by multiple S. aureus strains and a strong biofilm-forming strain of S. epidermidis. In addition, tilmicosin at three-fold of MIC disrupted USA300 mature biofilms and had a strong bactericidal effect on embedded bacteria. Furthermore, in a BioFlux flow biofilm assay, tilmicosin showed potent anti-biofilm activity and synergized with oxacillin against USA300.

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Antibiotic Susceptibility of Staphylococcus aureus Plankton and Biofilm Forms Isolated in Implant-Associated Infection

Cited by 4 publications

References 7 publications

The 2023 Orthopedic Research Society's international consensus meeting on musculoskeletal infection: Summary from the in vitro section

The 2023 Orthopedic Research Society's international consensus meeting on musculoskeletal infection: Summary from the in vitro section

Carbon-Infiltrated Carbon Nanotubes Inhibit the Development of Staphylococcus aureus Biofilms

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

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