Antibiotic Susceptibility of Enterohemorrhagic Escherichia coli O157:H7 Isolated from an Outbreak in Japan in 1996

Abstract

The antibiotic susceptibilities of 43 strains of Escherichia coli O157:H7 identified in the summer of 1996 in Japan were investigated. Growth of 90% of O157 strains was inhibited at a concentration of ≤0.5 μg/ml by several agents including fosfomycin with glucose-6-phosphate.

The effect of probiotic treatment withClostridium butyricumon enterohemorrhagicEscherichia coliO157:H7 infection in mice

“…The mainstay of the therapy for hemorrhagic colitis is the management of dehydration, electrolyte abnormalities and gastrointestinal blood loss. The antibiotic susceptibilities of EHEC have been investigated [33]. However, no controlled trials of antibiotic treatment of EHEC infection have been reported.…”

Perfil de susceptibilidad a los antimicrobianos en cepas de E coli productoras de Shiga toxina (STEC) aisladas de infecciones humanas y de alimentos

“…Otros estudios han demostrado que la exposición a concentraciones subinhibitorias de quinolonas 13 y cotrimoxazol 11 producirían una gran liberación de toxinas, probablemente por activación de la respuesta SOS con sobreexpresión de genes Stx 22 . En el extremo opuesto, otro estudio ha descrito que se produciría una disminución de la producción de Stx al utilizar norfloxacina, fosfomicina, kanamicina, ampicilina y claritomicina en modelo de ratón murino, en donde también se demostró disminución del riesgo de complicaciones así como disminución de la excreción de STEC y de la citotoxina en deposiciones y sangre 23 . Desde el punto de vista clínico, se ha descrito que ciertos antimicrobianos administrados precozmente en el curso de la infección podrían prevenir la progresión hacia SHU 14,15 .…”

Systematic review: are antibiotics detrimental or beneficial for the treatment of patients with Escherichia coli O157:H7 infection?

“…An in vitro investigation of antibiotic susceptibilities during the 1996 outbreak in Osaka, Sizuoka and Tokyo showed that the growth of 90% of 43 strains of E. coli O157 collected from 97160 stool specimens was inhibited at a concentration of ≤0.5 μ g/mL by 15 of 24 antimicrobial agents tested, which included new quinolones, cephalosporins (except cefaclor) and fosfomycin with 3 μ g/mL of G6P. G6P is released from serum into the intestine of patients with O157 and induces the transport of fosfomycin into the bacteria 47 …”

“…In 1996, several large outbreaks of EHEC O157:H7 infection occurred in Japan affecting more than 9000 people and causing 11 deaths 47 . Although treatment with antibiotics is not considered proven to be efficacious for this infection, the Japanese Ministry of Health and Welfare recommended that fosfomycin, norfloxacin or kanamycin be administered orally to children and that fosfomycin or a new quinolone be given to adults orally during the initial stage of infection 47 . A survey study targeting physicians who treated these patients revealed that most of the patients (95.9%) received antimicrobial agents and that fosfomycin comprised 84% of the prescribed treatment 32 …”