Antibiotic susceptibility of clinical isolates of Pseudomonas aeruginosa

Cervantes-Vega, C; Chávez, Jaime; Rodríguez, Manuel García

doi:10.1007/bf00428643

Cited by 11 publications

(5 citation statements)

References 15 publications

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“…Such resistance-modifying activity of the silver becomes quite important given P. aeruginosa is known rarely to be susceptible to augmentin [ 53 ], and augmentin resistance is quite prevalent among its clinical isolates [ 54 ]. Streptomycin-resistance was reported by [ 55 ] to be among the most common resistance patterns in P. aeruginosa clinical isolated from Mexico.…”

Section: Resultsmentioning

confidence: 99%

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Gajera,

Thakkar,

Godse

et al. 2023

BMC Microbiol

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Background Pseudomonas aeruginosa is a notorious pathogen. Its multidrug resistant strains are listed among priority pathogens against whom discovery of novel antibacterial agents and, elucidation of new anti-pathogenicity mechanisms are urgently warranted. This study describes multiple antibacterial effects of a colloidal nano-silver formulation- Silversol® against a multi-drug resistant strain of P. aeruginosa. Results Minimum inhibitory concentration (MIC) of Silversol® against P. aeruginosa was found to be 1.5 ppm; and at sub-MIC of 1 ppm, it was able to alter quorum-sensing regulated pigmentation (pyocanin 82%↓; pyoverdine 48%↑), exopolysaccharide synthesis (76%↑) and biofilm formation, susceptibility to antibiotics (streptomycin and augmentin), protein synthesis and export (65%↑), nitrogen metabolism (37%↑ nitrite accumulation), and siderophore production in this pathogen. Network analysis of the differentially expressed genes in the transcriptome of the silversol-treated bacterium identified ten genes as the potential molecular targets: norB, norD, nirS, nirF, nirM, nirQ, nosZ, nosY, narK1, and norE (all associated with nitrogen metabolism or denitrification). Three of them (norB, narK1, and norE) were also validated through RT-PCR. Conclusions Generation of nitrosative stress and disturbance of iron homeostasis were found to be the major mechanisms associated with anti-Pseudomonas activity of Silversol®.

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Section: Resultsmentioning

confidence: 99%

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Gajera,

Thakkar,

Godse

et al. 2023

BMC Microbiol

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“…B14-6 was susceptible to gentamicin (MIC 0.5 µg/mL), tobramycin (MIC 0.5 µg/mL), kanamycin (MIC 2 µg/mL), and apramycin (MIC 4 µg/mL) at 25 °C. However, this bacterium was resistant to streptomycin (MIC 32 µg/mL), which was comparable to the mesophilic Pseudomonas aeruginosa , often displays resistance to streptomycin ( Table 1 ) [ 21 , 22 ]. Taken together, these results indicate that Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides

et al. 2022

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Bacteria can evade antibiotics by acquiring resistance genes, as well as switching to a non-growing dormant state without accompanying genetic modification. Bacteria in this quiescent state are called persisters, and this non-inheritable ability to withstand multiple antibiotics is referred to as antibiotic tolerance. Although all bacteria are considered to be able to form antibiotic-tolerant persisters, the antibiotic tolerance of extremophilic bacteria is poorly understood. Previously, we identified the psychrotolerant bacterium Pseudomonas sp. B14-6 from the glacier foreland of Midtre Lovénbreen in High Arctic Svalbard. Herein, we investigated the resistance and tolerance of Pseudomonas sp. B14-6 against aminoglycosides at various temperatures. This bacterium was resistant to streptomycin and susceptible to apramycin, gentamicin, kanamycin, and tobramycin. The two putative aminoglycoside phosphotransferase genes aph1 and aph2 were the most likely contributors to streptomycin resistance. Notably, unlike the mesophilic Pseudomonas aeruginosa PA14, this cold-adapted bacterium demonstrated reduced susceptibility to all tested aminoglycosides in a temperature-dependent manner. Pseudomonas sp. B14-6 at a lower temperature formed the persister cells that shows tolerance to the 100-fold minimum inhibitory concentration (MIC) of gentamicin, as well as the partially tolerant cells that withstand 25-fold MIC gentamicin. The temperature-dependent gentamicin tolerance appears to result from reduced metabolic activity. Lastly, the partially tolerant Pseudomonas sp. B14-6 cells could slowly proliferate under the bactericidal concentrations of aminoglycosides. Our results demonstrate that Pseudomonas sp. B14-6 has a characteristic ability to form cells with a range of tolerance, which appears to be inversely proportional to its growth rate.

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“…However researchers have demonstrated the bactericidal activity of streptomycin against Pseudomonas aeroginosa [19] [28] but recent studies has demonstrated the developing streptomycin resistance in Pseudomonas aeruginosa [29] [30].…”

Section: Discussionmentioning

confidence: 99%

Effect of Combined Therapy on Colistin Resistant <i>Pseudomonas aeruginosa</i>: An <i>in Vitro</i> Study

Fatima¹,

Anwar²,

Madhkhali³

et al. 2019

AiM

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Pseudomonas aeruginosa is known for its antibiotic resistance to the clinicians. The infections caused by this pathogen are hard to treat because of its highly versatile property to mutate and acquire drug resistance. Pseudomonas aeruginosa also possesses intrinsic property of resistance to certain antibiotics like tetracyclines. However; in a practice to overcome the problem of multi drug resistance, clinicians restored the use of some antibiotics that were previously been used to treat the Pseudomonal infections; but they were discontinued because of its toxic effects. Colistin is an example of one such antibiotic. Use of Colistin was barred for its neurotoxicity. However in recent clinical trials, Colistin was reintroduced to fight with this superbug. Sadly in recent years, Pseudomonas aeruginosa developed resistance to Colistin as well. Therefore combined therapy is an alternate and suitable treatment to overcome the infections caused by multidrug-resistant Pseudomonas aeruginosa. The present study is an in vitro study; in which we tested synergy between two antibiotics namely streptomycin and Colistin on 29 clinical isolates of P. aeruginosa collected from hospitals in Jazan city KSA. The combination of two drugs showed synergistic activity on 55.1% of tested strains, while 20.6% strains had partial synergy, whereas indifferent synergy was observed in 13.8% strains and the 6.8% of strains had additive synergy. In addition to this, the drugs when combined also showed antagonism on one strain (3.44%). The present study showed synergistic action on Colistin-resistant Pseudomonas aeruginosa to greater extent (55.1%) by the two tested drugs. Hence Colistin and streptomycin can be used as a suitable combination therapy (in vivo) to treat multidrug resistant P. aeruginosa infections.

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Antibiotic susceptibility of clinical isolates of Pseudomonas aeruginosa

Cited by 11 publications

References 15 publications

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides

Effect of Combined Therapy on Colistin Resistant <i>Pseudomonas aeruginosa</i>: An <i>in Vitro</i> Study

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Antibiotic susceptibility of clinical isolates of Pseudomonas aeruginosa

Cited by 11 publications

References 15 publications

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Sub-lethal concentration of a colloidal nanosilver formulation (Silversol®) triggers dysregulation of iron homeostasis and nitrogen metabolism in multidrug resistant Pseudomonas aeruginosa

Arctic Psychrotolerant Pseudomonas sp. B14-6 Exhibits Temperature-Dependent Susceptibility to Aminoglycosides

Effect of Combined Therapy on Colistin Resistant &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;: An &lt;i&gt;in Vitro&lt;/i&gt; Study

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Effect of Combined Therapy on Colistin Resistant <i>Pseudomonas aeruginosa</i>: An <i>in Vitro</i> Study