Antibiotic susceptibility and toxins production of Staphylococcus aureus isolated from clinical samples from Benin

Abstract

A wide range of clinical samples were screened for identification of Staphylococcus aureus, their antibiotic sensitivity profile and the production of different leucotoxin and epidermolysins was evaluated. Out of 2,040 biological samples (collected from pus, urine, sperms, genital, catheter and blood of hospitalized and extra-hospital patients) screened, 123 pure cultures of S. aureus colonies were isolated. 48.78% of S. aureus were resistant to methicillin (MRSA), while 78% of them were isolated from extra-hospital patients. The S. aureus isolated from urines, pus and blood produced Panton and Valentine leukocidin (PVL) toxin, while the leucotoxin lukE-lukD was exclusively encountered by S. aureus isolated from pus samples. None of the bacterial colony isolated produced epidermolysin toxins A and B. In addition, 3.25% of MRSA and 8.13% of methicillin sensitive S. aureus (MSSA) produced PVL respectively. Our results indicated high frequency rate of MRSA in extra-hospital screened samples isolated from various types of infection. This high resistance rate combined with toxin production increases the virulence of S. aureus colonies and put therefore at risk the life of the patients in developing countries where auto-medication is not controlled. There is the need to instruct the population in order to avoid further widening of MRSA territory.

Prevalence and Antibiotic Sensitivity Pattern of Staphylococcus Aureus From All Clinical Samples With Emphasis on Mrsa in a Tertiary Care Hospital

“…Out of the 206, MRSA were 99 (48%) and MSSA were 107 (51.9%) as shown in [12,13,14,15] In the present study, the prevalence of S. aureus isolates in OPD, IPD and ICU was 49.0%, 40.3% and 10.7% respectively. The prevalence of MRSA was 48.5% in OPD, 48.2% in IPD and 45.5% in ICU.…”

“…Our study showed 36.4% resistance towards Gentamicin which was similar to a study conducted in South East Nigeria in 2016 by Chijioke A Nsofor et al where Gentamicin resistance was 31.7%. [13] Two other similar studies conducted by Dhanalakshmi TA et al [20] and Sina H et al [12] showed 33.6% and 42.27% resistance respectively towards Gentamicin. On the other hand, in present study, the resistance pattern for Ciprofloxacin was found to be 40.3%.…”

“…This could be the reason of non-uniform epidemiology of MRSA in India. [18] Very low prevalence of 5.26% and 6% were seen in studies from Peshawar in 2014 [19] and Benin in 2011 [12] respectively.…”

“…35.77%. [12] Some other researches have reported high resistance against Erythromycin. [13,17,20,21] In the ongoing study, 59.6% MRSA isolates and 7.5% MSSA isolates showed resistance against erythromycin.…”

“…[18] Similarly researches conducted in Benin, Bengaluru South East Nigeria showed a prevalence similar to the ongoing study. [12,15,13] A study was conducted by Indian network for Surveillance of Antimicrobial Resistance (INSAR) group, India in different leading hospitals of India to study the prevalence and susceptibility pattern of MRSA. The overall prevalence observed was 42% in 2008 and 40% in 2009 for MRSA.…”