Antibiotic susceptibility and plasmid profi le of heavy metal resistant Pseudomonas species

Abstract

Despite the important role of adiponectin in the progression of pathogenesis of liver cirrhosis, few data have been collected from the patients with different stages of liver diseases related to hepatitis B virus infection. We have studied the role of adiponectin in hepatitis B patients. Experiments were conducted an OPD-based cross-sectional study in a tertiary care hospital Assam, India which is a HBV-endemic country. A total of 220HBV-infected individuals and 110 healthy controls, were assessed for serum adiponectin levels which were quantifi ed by enzyme-linked immunosorbent assay. Serum HBV viral load and markers, serum alanine aminotransferase levels and metabolic factors and also the single nucleotide polymorphism of Adiponectin were analysed. Adiponectin levels were found to be associated with the chronic HBV infection (p<0.05).The presence of HBV infection was found to be positively associated with serum adiponectin levels(P < 0.0001).Out study has also reported that allele TG of SNPrs2241766 (p<0.05,OR=2.03),GT of rs1501299(p<0.05,OR=1.78) and CG of SNPrs266729 (p<0.05,OR=2.11) has a signifi cant effect on this disease. Study fi ndings were also expressed that, the serum adiponectin levels were positively associated with HBV viral load in overweight to HBV-infected subjects (p<0.05).The quantitative value of adiponectin was found higher in those patients with viral load of about 200×103-200×106 log copies/ml. Although the chronic HBV-infected individuals were more severe than the healthy controls, they were found as signifi cantly higher serum adiponectin levels than healthy persons. Also it was found that the adiponectin levels were positively associated with HBV viral load in overweight to obese HBV-infected subjects.It is now possible to suggest that serum adiponectin may have a role in the progression in fi brosis in CHB infection.

Heavy metal tolerance in association with plasmid mediated multiple antibiotic resistances among clinical bacterial isolates

“…Because the heavy metal (pollution) acts discriminatorily as a selective agent in the emergence and propagation of antibiotic resistance among bacteria, wherein, along with the genes conferring antibiotic resistance, metal tolerance genes are also encoded in the same plasmids (Foster, 1983;Fang et al, 2016). The clinical isolates of Pseudomonas spp., as reported by Rajasekar and Mohankumar (2016), had resistance to multiple antibiotics and heavy metals, and the resistance properties were shown to be plasmid (10 kb) mediated. A conjugative plasmid (≈56.4 kb), carrying resistance to multiple heavy metals, such as, Hg 2+ , Cu 2+ , Pb 2+ , Cd 2+ , and also antibiotics was detected among the isolates of E. coli and K. pneumoniae causing nosocomial infections (Karbasizaed et al, 2003).…”