Antibiotic susceptibilities of Yersinia enterocolitica and Y. intermedia isolates from aquatic environments

Abstract

Of 37 Yersinia isolates from various aquatic environments, seven were I: enterocolitica and 30 I: intermedia. These isolates were biotyped, serotyped and tested for their susceptibility to 20 antibiotics. All I: enterocolitica isolates were of biovar 1; those of I: intermedia were distributed amongst four biovars (1, 2, 4 and 6). On the basis of combined biotyping and serotyping results, Y. enterocolitica isolates were distributed in five and X intermedia in 17 groups. With the exception of one Y. enterocolitica isolate which was resistant to tetracycline and streptomycin, the isolates were sensitive to the non-P-lactam antibiotics. In contrast, various patterns of p-lactam insensitivity were detected, including ampicillin and ticarcillin (35 isolates), cephalothin (33 isolates), carbenicillin (32 isolates), amoxycillin/clavulanate (23 isolates) and cefoxitin (22 isolates). No correlation between biotype or serotype and the susceptibility pattern of the isolates was apparent. Both inducible cephalosporinase activity against third-generation cephalosporins and inhibition of resistance to penicillins were detected in all I: enterocolitica and Y. intermedia isolates by double-disk tests.

Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil

“…The majority of the 35 strains isolated from food (74?3 %) were resistant to FOX, as well as AMP, CEF and CFZ. The resistance profile obtained is in agreement with previous studies, in which resistance to AMP and many cephalosporins is frequently observed Tzelepi et al, 1999;White et al, 2002;Stock & Wiedemann, 2003). In fact, the expression of b-lactamase enzymes A and B has already been associated with Y. enterocolitica, Yersinia intermedia and Yersinia frederiksenii (Stock et al, , 2000Tzelepi et al, 1999).…”

Characteristics of β-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii

“…In the present study, Bla-B, the inducible cephalosporinase, was detected unequivocally based on the characteristic flattening of inhibition zone around cefotaxime disc adjacent to the imipenem disc in all the strains (Table 1). Tzelepi et al [17] also reported detection of a broad spectrum penicillinase (Bla-A) and an inducible cephalosporinase (Bla-B) in all the thirty aquatic isolates of Y. intermedia . Our study extends this information further to indicate that Bla-A was also present in Y. intermedia isolated from clinical and non-clinical (pig/wastewater) sources.…”

“…For ceftazidime, the MIC 50 and MIC 90 were 512 mg/L and 1024 mg/L respectively, and for cefepime, the values ranged from 256–1024 mg/L. Contrary to these, relatively lower MICs of penicillins and cephalosporins have been reported for Y. intermedia and Y. frederiksenii isolated in other parts of the world [13,17,18]. This may be due to difference in the sources from which organisms were isolated or their geographical origin.…”

“…This may be due to difference in the sources from which organisms were isolated or their geographical origin. Interestingly, Tzelepi et al [17] found that the strains of Y. intermedia isolated in Europe were sensitive to both cefotaxime and ceftazidime. The antibiotic resistance data of strains of Y. intermedia and Y. frederiksenii was in accordance with the preliminary reports from our laboratory earlier [19].…”

Kinetics activity of Yersinia Intermedia Against ZnO Nanoparticles Either Synergism Antibiotics by Double-Disc Synergy Test Method

“…As well, they are sensitive to a lower extent to the nalidixic acid, ampicillin, and amikacin (1,2,10,11). With regard to the tetracycline antibiotic, there were reports indicating diverse results through undertaking a set of different essays so far.…”