Antibiotic sensitivity pattern of indigenous lactobacilli isolated from curd and human milk samples

Abstract

The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host. This study evaluated the antibiotic susceptibility patterns of LAB isolated from curd (20) and human milk (11) samples. Antibiotic susceptibility was determined against 26 common antibiotics, following reference disc diffusion assay. A varied response in terms of susceptibility and resistance towards antibiotics was recorded. Among curd isolates, D7 (Lactobacillus plantarum) was the most resistant followed by D4, D8, D10 and D25. Among human milk isolates, HM-1 (L. casei) showed the highest resistance profile. All LAB isolates displayed high susceptibility pattern towards imipenem and meropenem. In general, high resistivity was exhibited by human milk isolates. The present study showed that antibiotic resistance is widespread among different lactobacilli, which may pose a food safety concern. Therefore, antibiotic sensitivity should be considered as a vital tool for safety assessment of probiotics.

Presence of blaCTX-M antibiotic resistance gene in Lactobacillus spp. isolated from Hirschsprung diseased infants with stoma

“…Regardless the availability of the clinical data about the resistance pattern of different antibiotics in Lactobacillus spp. ; not much is known about the presence of corresponding some other β-lactamases, carbapenemases and colistin resistance genes such as blaTEM, blaCTX-M, blaOXA-2, blaIMP, blaNDM-1, blaVIM-2 as well as mcr-1 in the same bacterial group [12,20,21]. Therefore, in the present study, antibiotic resistance pattern of Lactobacillus spp., isolated from HD infants was investigated through the disc diffusion method as well as molecular methods to check the presence of different antibiotic resistance genes.…”

“…To compare the results of the antibiotic susceptibility assay, the known standard described in the CLSI guideline (Clinical and Laboratory Standards Institute, 2015) was used for antimicrobial susceptibility testing as has been mentioned previously, where zone diameter > 20 mm was noted as susceptible, 15-19 mm as intermediate and ≤ 14 mm as resistant [20]. Table 3 shows the zone diameter of the six Lactobacillus spp.…”

In vitro Assessment of Antibiotic Resistance Pattern among Lactobacillus Strains Isolated from Goat Milk

“…from raw goat (Beetal) milk obtained from dairy farm of Guru Angad Dev Veterinary and Animal Sciences University (GADVASU) Ludhiana, Punjab, India. Milk samples were processed and lactobacilli were isolated and identified as discussed previously (Panwar et al, 2016;Sharma et al, 2017a and2017b …”

“…Ltd. Mumbai, India. The list of 26 antibiotics, their dosage and family had been summarized in our previous manuscript (Sharma et al, 2017a); except four new antibiotics namely, Azithromycin (Macrolides), Cefmetazole (2 nd generation cephalosporins), Cephalothin (1 st generation cephalosporins), and Amoxyclav (β-lactams).…”

“…The susceptibility pattern of different isolates and cultures were evaluated through modified Kirby-Bauer disc-diffusion assay (Sharma et al, 2017a). Isolates with ZOI≤14mm were recorded as resistant (R), ZOI≥20 as susceptible (S), and ZOI in between 15-19mm were considered as intermediate (I).…”