Antibiotic-Resistant
            <i>Escherichia coli</i>
            Bacteria, Including Strains with Genes Encoding the Extended-Spectrum Beta-Lactamase and QnrS, in Waterbirds on the Baltic Sea Coast of Poland

Literák, Ivan; Dolejská, Monika; Janoszowská, Dagmar; Hrusakova, Jolana; Meissner, Włodzimierz; Rzyska, Hanna; Bzoma, Szymon; Čížek, Alois

doi:10.1128/aem.01446-10

Cited by 143 publications

(134 citation statements)

References 82 publications

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“…In recent years, different reports have alerted us to the spread of VRE and/or ESBL-containing E. coli isolates from clinical, farm and wild animals in different European countries (Mallon et al, 2002;Torres et al, 2003;Bonnedahl et al, 2010;Literak et al, 2010;Pinto et al, 2010). Although the percentage of vanA isolates from wild birds in the Azores was lower when compared with other studies performed in mainland Portugal in farm and wild animals (Poeta et al, 2005a;Gonçalves et al, 2010;Radhouani et al, 2010b, Radhouani et al, 2010c, our isolates showed a phenotype of multi-resistance.…”

Section: Discussioncontrasting

confidence: 46%

“…In this study, the ESBLcontaining E. coli isolate, classified in the A phylogroup, was more sensitive to the antimicrobial compounds tested than ESBL-positive E. coli isolates recovered from other wild animals in mainland Portugal Poeta et al, 2009;Pinto et al, 2010;Radhouani et al, 2010a). Moreover, the b-lactamase genes detected in our isolate (bla CTX-M-14 and bla SHV-12 ) were also found in wild birds in Sweden (Bonnedahl et al, 2010) and Poland (Literak et al, 2010). How these wild birds acquired VRE and ESBL-containing E. coli is unclear.…”

Section: Discussionmentioning

confidence: 69%

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Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

et al. 2011

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To study the prevalence of vancomycin-resistant enterococci (VRE) and extended-spectrum b-lactamase (ESBL)-containing Escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the Azores Archipelago. Samples were spread on SlanetzÁBartley agar plates supplemented with 4 mg/l vancomycin and on Levine agar plates supplemented with 2 mg/l cefotaxime for VRE and ESBL-containing E. coli isolation, respectively. vanA-containing enterococcal isolates (four Enterococcus faecium and two Enterococcus durans) and vanC-1 Enterococcus gallinarum isolates were detected in six and seven faecal samples, respectively. VRE isolates showed ampicillin (n 011), ciprofloxacin (n 09), tetracycline (n06), erythromycin (n 05), quinupristin/dalfopristin (n 03) and high-level kanamycin resistance (n 01). The tet(L) and/or tet(M) gene was found in all tetracycline-resistant isolates and the erm(B) gene in all erythromycin-resistant isolates. Three vanAcontaining E. faecium and two E. gallinarum presented specific sequences of the Tn5397 transposon. Four VRE isolates harboured the ace virulence gene. One faecal sample revealed one ESBL-containing E. coli isolate that belongs to the A phylogenetic group, showed a phenotype of resistance to b-lactams and tetracycline, and harboured the bla CTX-M-14 , bla SHV-12 and the tet(A) genes. To our knowledge, this is the first study to focus on defining the prevalence of VRE and/or ESBL-containing E. coli strains in wild birds from the Azores. The data recovered are essential to improve knowledge about the dissemination of resistant strains through wild ecosystems and their possible implications by transferring these resistances to other animals or to humans.

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Section: Discussioncontrasting

confidence: 46%

Section: Discussionmentioning

confidence: 69%

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

et al. 2011

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“…Therefore, it is of general public interest to keep the number of coliform in the environment as low as possible. The E. coli strains isolated from the European population of P. carbo have not only been shown to be highly resistant to antibiotics such as cephalosporins and fluoroquinolones but the highly virulent O25b-ST131 clone has been identified among them (Literak et al, 2010;Tausova et al, 2012). Altogether, these findings indicate that cormorants may spread epidemiologically important antimicrobial-resistant bacteria through their droppings.…”

Section: Role In Pathogen Dispersionsupporting

confidence: 51%

The complexity of ecological impacts induced by great cormorants

Klimaszyk

Rzymski

2015

Hydrobiologia

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Following decades of global extermination, the general population of the great cormorant (Phalacrocorax carbo L.) is on the rise. The lack of regular predators, highly skilled fish rapacity, rapid metabolism, significant rate of excretion and ability to form large nesting colonies on relatively small areas lead to numerous environmental consequences of cormorant presence. Here we comprehensively review the occurrence and distribution of this species and, in particular, its multi-faceted impact on terrestrial and aquatic ecosystems and the main routes through which these impacts are being manifested. The bird-induced chemical loading and its biological and ecological consequences, and the effects on microbial pollution and pathogen dispersion are discussed in particular. The need for further investigation to fully elucidate particular effects is stressed throughout the paper. It is concluded that the environmental effects of great cormorants are rather complex, can lead to serious ecosystem modifications and that the presence of these birds should be taken into consideration in ecological assessment and monitoring.

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“…FQ and ESC resistant E. coli strains have been identified in wild birds (Allen et al, 2010;Literak et al, 2010) and from Australian wildlife rehabilitation centres (Blyton et al, 2015). Chapter 4 of this thesis identified wild birds as a potential risk factor associated with increased APEC carriage in healthy birds.…”

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Antibiotic-Resistant Escherichia coli Bacteria, Including Strains with Genes Encoding the Extended-Spectrum Beta-Lactamase and QnrS, in Waterbirds on the Baltic Sea Coast of Poland

Cited by 143 publications

References 82 publications

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

The complexity of ecological impacts induced by great cormorants

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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