Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

Smyth, Cian; O’Flaherty, Aidan; Walsh, Fiona; Thuy, Thi

doi:10.1016/j.envpol.2020.114244

Cited by 29 publications

(13 citation statements)

References 73 publications

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“…This finding compares with the results of our study, where the blaTEM gene was found in 97% of ESBL isolates obtained from the secondary treatment stage, whereas only 42.8% isolates had the blaSHV gene. A recent study found very low percentages of the ESBL genes in 11.84% of isolates obtained from wastewater effluent samples, and of these, only 9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15 [45].…”

Section: Discussionmentioning

confidence: 94%

Occurrence of Extended Spectrum Beta-Lactamase Gram-Negative Bacteria from Non-Clinical Sources in Dubai, United Arab Emirates

Khan

Thurgood

Faheem

et al. 2020

Water

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Extended-spectrum beta-lactamase (ESBL) producing bacteria of the Enterobacteriaceae family are a significant threat to public health, posing a challenge for health authorities worldwide. In the UAE, very little information is available about ESBL producing bacteria from non-clinical sources. In this study, 206 pure cultures belonging to the Enterobacteriaceae family were isolated from food and wastewater sources in Dubai, UAE. All the isolates were tested against third-generation cephalosporin antibiotics by the disc diffusion method and screened on ESBL chromogenic agar. Among all isolates (n = 86), 41.7% were potential ESBL producers belonging to E. coli, Klebsiella, Enterobacter, Shigella, and Citrobacter (KESC group), and Proteus. Of all the potential ESBL producing isolates, 19 (22%) were confirmed as ESBL producers by a double-disc diffusion test with the fourth generation cephalosporin–Cefpirome. The multiplex polymerase chain reaction was used for the detection of ESBL bla genes in the screened isolates. Out of a total of 86 isolates, 52.3% possessed only the blaTEM gene; 39.5% contained both blaTEM and blaSHV genes, while only 3.5% contained the blaCTX-M gene. The carbapenemase resistance test showed eight isolates resistant to imipenem, and only one isolate with metallo-beta-lactamase activity. This study highlights the occurrence of ESBL bla genes among non-clinical isolates from food and wastewater sources in the UAE and emphasizes the importance of food and wastewater surveillance programs in controlling the spread of antibiotic resistance.

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Section: Discussionmentioning

confidence: 94%

Occurrence of Extended Spectrum Beta-Lactamase Gram-Negative Bacteria from Non-Clinical Sources in Dubai, United Arab Emirates

Khan

Thurgood

Faheem

et al. 2020

Water

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“…The study of resistance to the antibiotic showed the presence of many resistance genes to beta-lactams whose resistance genes (TEM, SHV, CTX-M 15, NDM, VIM for Gram-negative bacilli and Mec A for Gram Positive Cocci), aminoglycosides (AADA) and glycopeptides (VAN A and Van B), sulfonamides (SUL 1 and SUL 2). Numerous studies have shown the presence of these resistance genes in bacteria isolated from water sources such as hospital wastewater and groundwater [8,38], drinking water [30,39,40]. The presence of certain multi-resistant bacteria and the absence of others in drinking water following the street sellers is the problem of distribution conditions of these drinking water bags.…”

Section: Discussionmentioning

confidence: 99%

“…Many matrices have been targeted as a source of dissemination of multidrug resistant bacteria in the environment and to humans, but water resources constitute an important source of dissemination of these multidrug resistant bacteria [7]. E uent hospital liquid, passing by piping waters and drinking water have been implicated as a vehicle for the spread of multidrug-resistant bacteria [8]. Integrated management of water resources would reduce the spread of pathogens both in hospitals and in the community [9].…”

Section: Introductionmentioning

confidence: 99%

Emergence of Multidrug-resistance Bacteria Isolated of Drinking Water, Groundwater and Hospital Wastewater in Southern Benin: Antibiotic Resistance Profile and Resistomes Determination

H¹,

Dougnon²,

Lougbegnon³

et al. 2021

Preprint

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Background: The environment plays an important role in the dissemination of multidrug resistant bacteria, especially through the aquatic ecosystem, which is made up of liquid hospital effluents, rivers, but also spring water and drinking water. This study aims to determinate resistomes in some aquatic matrices in southern Benin.Methods: These samples were filtered through a membrane 0.22 µm thick. After filtration, the membrane was deposited on Muëller Hinton agar. Then the colonies resulting from this subculture were subjected to a microbiological examination by the conventional method. The antibiotic sensitivity test was carried out by the Kirby Bauer method according to the recommendations of the French Society of Microbiology. Resistance genes were looked for by standard PCR and the PCR products were used for agarose gel electrophoresis.Results: Of the 222 water samples collected, 265 bacterial strains were isolated, the majority of which were strains of Coagulase Negative Staphylococcus (CNS) with 37.74% (n = 100), followed by strains of Klebsiella pneumoniae (21.89%; n = 58), Escherichia coli (10.57%; n = 28). All isolated gram-negative bacilli strains are multidrug resistant with resistance of all strains to amoxicillin, ampicillin and amoxicillin + clavulanic acid. Of the 15 resistance genes sought in the genome of Gram-negative bacilli strains, only 8 were detected, namely the TEM, SHV, CTX-M15, VIM, NDM, SUL1, SUL2 and AADA genes. Strong resistance of CNS strains to amoxicillin, oxacillin and cefoxitin was observed. The Mec A gene was detected in all CNS strains. While the Van A and Van B genes were only detected in strains isolated from drinking water in sachets collected from producers and street sellers.Conclusion: These results show the dissemination of resistance genes in the aquatic ecosystem of Benin and once again confirms the urgency of a global fight against antimicrobial resistance.

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“…However, bla SHV and/or bla TEM were found frequently as well [ 15 , 82 , 83 , 84 , 85 ]. In some studies, bla TEM was predominant, e.g., in an Irish study [ 86 ], as well as in Colombia [ 87 ], Poland and Portugal [ 9 , 88 ], Belgium [ 89 ], the US [ 90 ], and Africa [ 91 ]. Another significant group representing the KPC family encoded by bla KPC genes was detected in numerous WWTPs from European [ 65 , 69 , 89 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 ], as well as from American [ 90 , 102 , 103 , 104 ], African [ 72 , 91 , 105 ], and Asian countries [ 106 , 107 ].…”

Section: Clinically Significant β-Lactam Resistance Genes In Wastewat...mentioning

confidence: 99%

Clinically Relevant β-Lactam Resistance Genes in Wastewater Treatment Plants

Waśko

Kozińska

Kotlarska

et al. 2022

IJERPH

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Antimicrobial resistance (AMR) is one of the largest global concerns due to its influence in multiple areas, which is consistent with One Health’s concept of close interconnections between people, animals, plants, and their shared environments. Antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) circulate constantly in various niches, sediments, water sources, soil, and wastes of the animal and plant sectors, and is linked to human activities. Sewage of different origins gets to the wastewater treatment plants (WWTPs), where ARB and ARG removal efficiency is still insufficient, leading to their transmission to discharge points and further dissemination. Thus, WWTPs are believed to be reservoirs of ARGs and the source of spreading AMR. According to a World Health Organization report, the most critical pathogens for public health include Gram-negative bacteria resistant to third-generation cephalosporins and carbapenems (last-choice drugs), which represent β-lactams, the most widely used antibiotics. Therefore, this paper aimed to present the available research data for ARGs in WWTPs that confer resistance to β-lactam antibiotics, with a particular emphasis on clinically important life-threatening mechanisms of resistance, including extended-spectrum β-lactamases (ESBLs) and carbapenemases (KPC, NDM).

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Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

Cited by 29 publications

References 73 publications

Occurrence of Extended Spectrum Beta-Lactamase Gram-Negative Bacteria from Non-Clinical Sources in Dubai, United Arab Emirates

Occurrence of Extended Spectrum Beta-Lactamase Gram-Negative Bacteria from Non-Clinical Sources in Dubai, United Arab Emirates

Emergence of Multidrug-resistance Bacteria Isolated of Drinking Water, Groundwater and Hospital Wastewater in Southern Benin: Antibiotic Resistance Profile and Resistomes Determination

Clinically Relevant β-Lactam Resistance Genes in Wastewater Treatment Plants

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