Antibiotic Resistance Profiling of Pathogenic Staphylococcus Species from Urinary Tract Infection Patients in Benin

Abstract

Staphylococci can cause urinary tract infections (UTIs). These UTIs are among the significant causes of antibiotic resistance and the spread of antibiotic-resistant diseases. The current study is aimed at establishing a resistance profile and determining the pathogenicity of Staphylococcus strains isolated from UTI samples collected in Benin. For this purpose, urine samples (one hundred and seventy) that were collected from clinics and hospitals showed UTI in patients admitted/visited in Benin. The biochemical assay method was used to identify Staphylococcus spp., and the disk diffusion method tested the antimicrobial susceptibility. The biofilm formation ability of the isolates of Staphylococcus spp. was investigated by the colorimetric method. The presence of mecA, edinB, edinC, cna, bbp, and ebp genes was examined by multiplex polymerase chain reaction (PCR). The results showed that Staphylococcus species were identified in 15.29% of all infected individuals and that 58% of these strains formed biofilms. Most Staphylococcus strains (80.76%) were isolated in female samples, and the age group below 30 years appeared to be the most affected, with a rate of 50%. All Staphylococcus strains isolated were 100% resistant to penicillin and oxacillin. The lowest resistance rates were seen with ciprofloxacin (30.8%), gentamicin, and amikacin (26.90%). Amikacin was the best antibiotic against Staphylococcus strains isolated from UTIs. The isolates carried mecA (42.31%), bbp (19.23%), and ebp (26.92%) genes in varying proportions. This study provides new information on the risks posed to the population by the overuse of antibiotics. In addition, it will play an essential role in restoring people’s public health and controlling the spread of antibiotic resistance in urinary tract infections in Benin.

Insight the Medicinal Plants Used to Treat Urinary Tract Infections in Benin

“…The study was conducted in six localities of Benin, focusing on the main cities within each. These cities were selected based on a prior study that examined the pathogenicity of urinary tract infections in Benin 9,10 . The towns included Cotonou in the Littoral department, Abomey-Calavi in the Atlantic Department, Lokossa in the Couffo Department and Abomey in the Zou Department.…”

“…Over 404.6 million people worldwide are affected by UTIs, with developing countries being the main targets and prevalence varying among different population groups 8 . In Benin, UTIs are a common cause of consultations and hospitalizations 9,10 and over 80% of the population relies on traditional medicine for their healthcare needs, it is crucial to highlight the prevalence of UTIs in the region and the importance of alternative remedies like medicinal plants.…”