Antibiotic Resistance Profile of Bacteria Isolated from Stream Water in Bokkos Local Government Area of Plateau State, Nigeria

Abstract

Water from selected streams in Bokkos local government area of Plateau State Nigeria was obtained and analysed for the presence of antibiotic resistant bacteria and the resistance pattern of the isolates against several antibiotics. A total of 25 stream water samples were collected for testing from five (5) different study sites (Tudu, Mabel, Dairy Farm, Maihakorin Gold and Kuba). Heterotrophic and coliform bacteria were isolated from the samples using 10-fold serial dilution and the pour plate method. The isolated bacteria were Proteus vulgaris, Salmonella spp., Escherichia coli, Pseudomonas spp., Shigella spp., Bacillus spp., Streptococcus spp., Staphylococcus aureus. Antibiotic susceptibility of isolates was determined using the disc diffusion method. Among the eight bacterial isolates, Salmonella spp. was the most frequently occurring bacterium 18(22.8%), followed by E. coli 16(20.3%), while the least occurring bacterium was Staphylococcus aureus 5(6.3%). The highest antibiotic resistance was recorded for Salmonella spp., E. coli, and Pseudomonas spp. against ceftazidime 12(67%), Ampicillin 12 (75%), and ceftazidime 11 (92%), respectively. However, E. coli, Proteus spp., Staphylococcus spp., and Shigella spp. showed high antibiotic susceptibility against cefuroxime 0(0%) and Gentamicin 0(0%). The results suggests that there are antibiotic resistance bacteria in stream water in Bokkos local government area of Plateau State. Educational awareness regarding the proper use of safe drinking water and the treatment of water before use should be encouraged.