Antibiotic resistance, phylogenetic grouping and virulence potential of Escherichia coli isolated from the faeces of intensively farmed and free range poultry

Obeng, Akua Serwaah; Rickard, Heather; Ndi, Olasumbo; Sexton, Margaret; Barton, Mary

doi:10.1016/j.vetmic.2011.07.010

Cited by 91 publications

(94 citation statements)

References 30 publications

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“…Increased use of fluoroquinolones instead of older-generation antimicrobial agents leads to gradual emergence of bacterial strains resistant to these antibacterials (Kurutepe et al, 2005). Our results are close to results of Obeng et al (2012), who have not found increased resistance to fluoroquinolones in E. coli isolates from healthy commercial and free-range chickens in Australia.…”

Section: Discussionsupporting

confidence: 91%

Antimicrobial susceptibility of Escherichia coli isolated from free range poultry or wild birds at the southern Caspian Sea coast of Iran

Seifi¹,

Shirzad²

2017

J Hellenic Vet Med Soc

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ABSTRACT. Objective of this study was the investigation of antimicrobial resistance of 95 Escherichia coli strains from faecal samples of wild birds and free range poultry in Mazandaran province (southern Caspian Sea coast of Iran), which were isolated during the period May to December 2012. Isolation rate was 59% or 30% from samples of free range poultry or wild birds, respectively. All isolates from free range poultry were susceptible to amikacin, danofloxacin, enrofloxacin, gentamicin, neomycin, whereas all were resistant to carbenicillin and erythromycin. In strains from wild birds, most common resistance was detected to be to carbenicillin and tetracycline. Multi-drug resistance was evident in all isolates from free range poultry and in 26% of those from wild birds. It is postulated that that wild birds may be infected by antibiotic resistant strains of E. coli and may serve as reservoirs of such strains.

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Section: Discussionsupporting

confidence: 91%

Antimicrobial susceptibility of Escherichia coli isolated from free range poultry or wild birds at the southern Caspian Sea coast of Iran

Seifi¹,

Shirzad²

2017

J Hellenic Vet Med Soc

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“…Clinical isolates of ExPEC typically belong to phylogenetic group B2 and, to a lesser extent, group D [32]. In agreement with several authors [33][34][35], we observed a link between phylogenetic groups and extraintestinal pathogenic strains because the majority of the strains belonged predominantly to phylogenetic group B2 and, to a lesser extent, to group D, whereas they were sparsely represented within groups A and B1. Strains belonging to group B2 harboured a greater number of virulence factors compared to strains from other phylogenetic groups, suggesting a putative association between virulence factors and pathogenic potential [36].…”

Section: Discussionsupporting

confidence: 90%

“…Previous data suggest that among E. coli isolates from patients with urosepsis, resistance to antimicrobial agents such as ampicillin, sulphonamides, tetracycline, and streptomycin is negatively associated with virulence [41]. More recent data demonstrate a similar negative association between antibiotic resistance and virulence factors or a B2 phylogenetic background [33,34,42]. This has been interpreted as loss of virulence factors concomitant with mutation to resistance [43].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance and virulence genes of extraintestinal pathogenic Escherichia coli from tropical estuary, south India

Sukumaran

Hatha

2015

J Infect Dev Ctries

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Introduction: Escherichia coli strains can cause a variety of intestinal and extraintestinal diseases. Extraintestinal pathogenic E. coli (ExPEC) strains have the ability to cause severe extraintestinal infections. Multidrug resistance among ExPEC could complicate human infections. Methodology: Escherichia coli strains were isolated during the period of January 2010 to December 2012 from five different stations set at Cochin estuary. Susceptibility testing was determined by the disk-diffusion method using nine different antimicrobial agents. A total of 155 strains of Escherichia coli were screened for the presence of virulence factor genes including papAH, papC, sfa/focDE, iutA,and kpsMT II associated with ExPEC. Results: Among the 155 E. coli isolates, 26 (16.77%), carried two or more virulence genes typical of ExPEC. Furthermore, 19.23% of the ExPEC isolates with multidrug resistance were identified to belong to phylogenetic groups B2 and D. Statistically significant association of iutA gene in ExPEC was found with papC (p < 0.001) and kpsMT II (p < 0.001) genes. ExPEC isolates were mainly resistant to ampicillin (23.07%), tetracycline (19.23%), co-trimoxazole (15.38%), and cefotaxime (15.38%). The adhesion genes papAH and sfa/focDE were positively associated with resistance to gentamicin, chloramphenicol, and cefotaxime (p < 0.05). Conclusions: Co-occurrence of virulence factor genes with antibiotic resistance among ExPEC poses considerable threat to those who use this aquatic system for a living and for recreation.

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“…This population of gut E. coli that contain APEC-associated VGs pose a risk for birds as they have the potential to cause avian colibacillosis Kemmett et al, 2013;McPeake et al, 2005). In the one Australian study, which screened 251 AFEC isolates cultured from healthy chickens for the presence of eight ExPEC-associated VGs (papAH, papC, afa/draBC, sfa/focDE, sfaS, aerJ, kpsMT II and focG), the authors reported that 10% of AFEC isolates carried two or more of the eight VGs and 68.1% harboured none of the VGs (Obeng et al, 2012). The detection of ExPEC-associated VGs among Australian AFEC isolates may pose a public health concern, as these isolates may act as a reservoir for the VGs.…”

Section: Commensal E Colimentioning

confidence: 99%

“…Globally, the economic, public health and animal welfare significance of avian colibacillosis, has led to a number of studies from United States of America aiming to define APEC and to identify pathogenicity mechanisms (Jeffrey et al, 1999;Kariyawasam and Nolan, 2009;Norton et al, 2000). Studies have identified a large number of VGs in E. coli cultured from birds with avian colibacillosis (Antão et al, 2008;Dissanayake et al, 2014; and relevance of these VGs in the pathogenicity of avian colibacillosis is still poorly understood and the majority of studies on APEC and APEC-associated VGs are descriptive (Dozois et al, 1994;Jeffrey et al, 1999;Norton et al, 2000;Obeng et al, 2012;Zhao et al, 2009)…”

Section: Avian Pathogenic E Coli (Apec)mentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Antibiotic resistance, phylogenetic grouping and virulence potential of Escherichia coli isolated from the faeces of intensively farmed and free range poultry

Cited by 91 publications

References 30 publications

Antimicrobial susceptibility of Escherichia coli isolated from free range poultry or wild birds at the southern Caspian Sea coast of Iran

Antimicrobial susceptibility of Escherichia coli isolated from free range poultry or wild birds at the southern Caspian Sea coast of Iran

Antibiotic resistance and virulence genes of extraintestinal pathogenic Escherichia coli from tropical estuary, south India

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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