Antibiotic resistance patterns of &lt;i&gt;Frankia&lt;/i&gt; strains

Tisa, Louis S.; Chval, Matthew S.; Krumholz, Glenn D.; Richards, Joel

doi:10.1139/cjb-77-9-1257

Cited by 11 publications

(12 citation statements)

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“…In addition to environmental stresses described above, the response of Frankia to other chemical and organic stressors such as antibiotics, phenolics, peroxides and hydrocarbons has been studied (Perradin et al 1983;Savouré and Lim 1991;Alskog and Huss-Danell 1997;Dobritsa 1998;Tisa et al 1999;Mallet and Roy 2014).…”

Section: Response To Other Chemical and Organic Stressorsmentioning

confidence: 99%

Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in actinorhizal plants adaptation

et al. 2016

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Environmental stresses are caused by human activities or natural events. Several of them including salinity, heavy metals, and extreme temperature affect both soil characteristics and plant growth and productivity. Actinorhizal plants are pioneer species that are able to grow in poor soils and improve soil fertility. They are widely used in agroforestry for different purposes including reclamation of degraded and contaminated lands. This capacity is mainly due to the plants forming a nitrogen-fixing symbiosis with actinobacteria known as Frankia. In comparison to uninoculated plants, plants in symbiosis with Frankia have significantly improved plant growth, total biomass, and nitrogen and chlorophyll content which enhance the development of actinorhizal plants and their resistance to abiotic stresses. However, to optimize the adaptation of actinorhizal species to different environments, selection of both symbiotic partners is necessary. Frankia strains vary in their sensitivity and response to stress including salinity, heavy metals, extreme pH and drought. In this paper, we review the response of different Frankia strains to environmental stresses and their role that they play in the adaptation of actinorhizal plants to stressful conditions.

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Section: Response To Other Chemical and Organic Stressorsmentioning

confidence: 99%

Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in actinorhizal plants adaptation

et al. 2016

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“…Stock cultures were grown and maintained in basal MOPS, Phosphate and Nitrogen (MPN) growth buffer (Tisa et al 1983(Tisa et al , 1999 (consisting of 20 mM 3-(N-morpholino)propanesulfonic acid (MOPS), 10 mM K 2 HPO 4 , and 5.0 mM NH 4 Cl as a nitrogen source, adjusted to pH 6.8) supplied with mixed metals (1 ml/l of trace salt mixture containing 0.5…”

Section: Frankia Strains and Growth Conditionsmentioning

confidence: 99%

Copper tolerance in Frankia sp. strain EuI1c involves surface binding and copper transport

Rehan

Furnholm

Finethy

et al. 2014

Appl Microbiol Biotechnol

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Several Frankia strains have been shown to be copper-tolerant. The mechanism of their copper tolerance was investigated for Frankia sp. strain EuI1c. Copper binding was shown by binding studies. Unusual globular structures were observed on the surface of the bacterium. These globular structures were composed of aggregates containing many relatively smaller "leaf-like" structures. Scanning electron microscopy with energy-dispersive X-ray (SEM-EDAX) analysis of these structures indicated elevated copper and phosphate levels compared to the control cells. Fourier transform infrared spectroscopy (FTIR) analysis indicated an increase in extracellular phosphate on the cell surface of copper-stressed cells. Bioinformatics' analysis of the Frankia sp. strain EuI1c genome revealed five potential cop genes: copA, copZ, copC, copCD, and copD. Experiments with Frankia sp. strain EuI1c using qRT-PCR indicated an increase in messenger RNA (mRNA) levels of the five cop genes upon Cu(2+) stress. After 5 days of Cu(2+) stress, the copA, copZ, copC, copCD, and copD mRNA levels increased 25-, 8-, 18-, 18-, and 25-fold, respectively. The protein profile of Cu(2+)-stressed Frankia sp. strain EuI1c cells revealed the upregulation of a 36.7 kDa protein that was identified as FraEuI1c_1092 (sulfate-binding periplasmic transport protein). Homologues of this gene were only present in the genomes of the Cu(2+)-resistant Frankia strains (EuI1c, DC12, and CN3). These data indicate that copper tolerance by Frankia sp. strain EuI1c involved the binding of copper to the cell surface and transport proteins.

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“…Frankia strains CcI3 (Zhang et al 1984), EAN1pec (Lalonde et al 1981) and EuI1c (Baker et al 1980) were grown and maintained in basal medium with NH 4 Cl as the nitrogen source, as described previously (Tisa et al 1999). For strain EuI1c, 20 m M glucose was used as carbon and energy source, while 20 m M fructose was used for strain EAN1pec.…”

Section: Methodsmentioning

confidence: 99%

Development of a physical map for three Frankia strains and a partial genetic map for Frankia EuI1c

Rawnsley

Tisa

2007

Physiologia Plantarum

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Physical maps for Frankia strains CcI3, EAN1pec and EuI1c chromosomes were constructed by the use of macrorestriction analysis and pulsed-field gel electrophoresis (PFGE). The restriction enzymes AseI, PmeI, SwaI and SspI were used to cut the Frankia chromosome into a limited number of large fragments and for double digestions. The genomes sizes, as determined by the addition of the estimated fragment sizes, were 5430 AE 35 kb, 9101 AE 109 kb and 8105 AE 842 kb for strains CcI3, EAN1pec and EuI1c, respectively. A complete physical map was achieved by the analysis of PFGE for the single and double digestions and by two-dimensional PFGE to determine doublets and overlapping fragments. For strain EuI1c, a partial genetic map was also constructed by positioning the 16S rRNA, glnII, glnA and hboO genes on the physical map. PFGE analysis of DNA with and without proteinase K treatment together with the other results suggested a circular genome.

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Antibiotic resistance patterns of <i>Frankia</i> strains

Cited by 11 publications

References 0 publications

Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in actinorhizal plants adaptation

Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in actinorhizal plants adaptation

Copper tolerance in Frankia sp. strain EuI1c involves surface binding and copper transport

Development of a physical map for three Frankia strains and a partial genetic map for Frankia EuI1c

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