Antibiotic Resistance Patterns and Pigment Production of Streptococci of Serological Group B Isolated from Bovines and Humans

Wibawan, I Wayan Teguh; Lautrou, Y.; Lämmler, Christoph

doi:10.1111/j.1439-0450.1991.tb00936.x

Cited by 14 publications

(16 citation statements)

References 27 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification of the bacteria was also conducted by PCR amplification of species‐specific parts of the gene encoding the 16S rRNA, the 16S–23S rDNA intergenic spacer region and the CAMP factor gene cfb with oligonucleotide primers and thermal cycler programs described previously [10,11]. Additional properties of the bacteria investigated were the determination of bacterial surface hydrophobicity by salt aggregation and hexadecane adherence tests [12], the ability to hemagglutinate rabbit erythrocytes [13], hyaluronate lyase enzyme activity [14], supported by PCR amplification of the corresponding gene hylB [15], and resistance reactions to various antibiotics [16].…”

Section: Methodsmentioning

confidence: 99%

Determination of epidemiological relationships ofStreptococcus agalactiaeisolated from bovine mastitis

Merl¹,

Abdulmawjood²,

Lämmler³

et al. 2003

FEMS Microbiology Letters

View full text Add to dashboard Cite

In the present study 79 streptococcal cultures isolated from subclinical mastitis of 54 cows from seven dairy farms (A^G) in Hesse, Germany, were comparatively investigated using conventional and molecular methods. The isolates could be identified as Streptococcus agalactiae, belonging to Lancefield's serological group B by determination of cultural, biochemical and serological properties and by polymerase chain reaction (PCR)-mediated amplification of species-specific parts of the 16S ribosomal DNA, the 16S^23S rDNA intergenic spacer region and the CAMP factor gene cfb. The investigated group B streptococci were further characterized serologically for specific polysaccharide and protein antigens. Serotyping the isolates revealed a predominance of surface protein antigen X, either alone or in combination with polysaccharide antigen Ia. This could be observed for 39 isolates of farms A, B and C. Six group B streptococci from farm E displayed the serotype pattern III/Rib, two isolates from farm G showed the serotype pattern Ib/cK. The remaining cultures from farms D and F (n = 32) were non-typable. The occurrence of protein Rib could be confirmed by PCR amplification of the gene rib. The two isolates with serotype pattern Ib/cK also reacted positively for the cL-encoding gene bag. Additional properties which allowed a phenotypic characterization of the S. agalactiae were the degree of pigmentation, growth properties in fluid media and soft agar, the surface hydrophobicity, the ability to hemagglutinate rabbit erythrocytes and their resistance reactions to tetracycline and minocycline. The isolates of the seven farms showed identical or almost identical characteristics. The 79 group B streptococci were additionally investigated by macrorestriction analysis of their chromosomal DNA using the restriction endonucleases SmaI, ApaI and SalI. The restriction patterns obtained by pulsed-field gel electrophoresis displayed identical or closely related patterns for the cultures of the various farms. The pheno-and genotypic characteristics of the 79 group B streptococci of the present study revealed that a single S. agalactiae strain or at least closely related subtypes of this strain were responsible for the mastitis situation of the seven farms.

show abstract

Section: Methodsmentioning

confidence: 99%

Determination of epidemiological relationships ofStreptococcus agalactiaeisolated from bovine mastitis

Merl¹,

Abdulmawjood²,

Lämmler³

et al. 2003

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…In parallel the bacteria were determined for biochemical properties, for colony morphology in soft agar media [30,21], for pigmentation in GBS Islam agar (Oxoid, Wesel, Germany) supplemented with 50 ml l 31 sterile horse serum [31], for hemagglutinating properties [32], for CAMP reactivity on sheep blood agar in the presence of a L toxin producing S. aureus, for antibiotic resistancies [31], and for hyaluronidase enzyme activity with a decapsulation test using a mucoid growing Staphylococcus equi subsp. zooepdemicus strain [29].…”

Section: Methodsmentioning

confidence: 99%

“…Finally 2.5 Wl DNA preparation was added to each reaction tube. For DNA preparation ¢ve to 10 colonies of the bacteria were suspended in 100 Wl TE bu¡-er containing mutanolysin (50 U; Sigma, Deisenhofen, Germany) for 60 min at 37 ‡C and treated with 10 Wl proteinase K (14.8 mg ml 31 ; Roche Diagnostics, Mannheim, Germany) for 120 min at 56 ‡C. The suspension was subsequently boiled for 10 min at 100 ‡C, centrifuged (10 000Ug, 5 s) and cooled before use.…”

Section: Methodsmentioning

confidence: 99%

Pheno- and genotypic properties of streptococci of serological group B of canine and feline origin

Yildirim

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

In the present study streptococci of serological group B isolated from canines (n = 48) and felines (n = 7) were comparatively investigated with group B streptococci from humans and bovines for cultural, biochemical and serological properties for antibiotic resistancies and by molecular analysis. An identification was performed with group B-specific antiserum, biochemical reactions, by PCR amplification and subsequent endonuclease digestion of the 16S rRNA gene and by amplification of species-specific parts of the 16S rDNA the 16S^23S rDNA intergenic spacer region and the CAMP factor gene cfb. Phenotypic similarities of group B streptococci of canine and feline origin with group B streptococci from humans and differences to group B streptococci of bovine origin could be observed in lactose fermentation, serotype patterns, pigmentation, growth properties of the bacteria in fluid medium and soft agar, hemagglutination reactions and in minocycline and tetracycline resistance. A negative hyaluronidase plate test, a hylB amplicon with a size of 4.6 kb and an insertion sequence 1548 could be observed among canine, feline and human group B streptococci of serotype III. The remaining hyaluronidase positive strains, also including all isolates of bovine origin, had a hylB gene with a size of 3.3 kb. Further genotypic differences could be observed in the occurrence of the genes lmb and scpB which appeared generally among canine, feline and human group B streptococci, but less pronounced among bovine isolates of this species. According to the presented data group B streptococci of canine and feline origin seemed to be more related to human than to bovine isolates of this species possibly indicating some epidemiological relation. ß

show abstract

“…Direct transmission of group B streptococci between bovines and humans remains uncommon (5,20). Cultural, biochemical, and serological characteristics supported the conclusion that human and bovine isolates form two distinct biotypes (9,16,27). However, group B streptococci have also been isolated from other domestic animals (occasionally from pathological material) (7,11,15,18,23,29).…”

mentioning

confidence: 66%

“…Antibiotic susceptibility. The determination of antibiotic susceptibility was performed as described previously (27).…”

mentioning

confidence: 99%

Properties and type antigen patterns of group B streptococcal isolates from pigs and nutrias

1993

View full text Add to dashboard Cite

All 59 group B streptococcal cultures isolated from pigs and nutrias reacted with group B-specific antiserum and gave a positive CAMP reaction in the zone of staphylococcal beta-lysin. Most of the cultures were pigmented; all cultures hydrolyzed Na hippurate and utilized salicin, maltose, and saccharose but not esculin, mannitol, or inulin. Fifty-three percent of the group B streptococci from pigs and none of those from nutrias were lactose positive. Serotyping revealed that most of the group B streptococci from pigs were of serotype III and most of those from nutrias were of type Ia/c. Protein c was present as c3 antigen. All group B streptococci were susceptible to penicillin and bacitracin (10 U), and most of the porcine cultures were resistant to tetracycline. According to these results, group B streptococci from pigs and nutrias differ from bovine and human group B streptococci and seem to play no role in cross-infections between animals or between animals and humans.

show abstract

Antibiotic Resistance Patterns and Pigment Production of Streptococci of Serological Group B Isolated from Bovines and Humans

Cited by 14 publications

References 27 publications

Determination of epidemiological relationships ofStreptococcus agalactiaeisolated from bovine mastitis

Determination of epidemiological relationships ofStreptococcus agalactiaeisolated from bovine mastitis

Pheno- and genotypic properties of streptococci of serological group B of canine and feline origin

Properties and type antigen patterns of group B streptococcal isolates from pigs and nutrias

Contact Info

Product

Resources

About