Antibiotic resistance pattern and phylogenetic groups of the uropathogenic Escherichia coli isolates from urinary tract infections in Hamedan, west of Iran

Abstract

Background and Objectives: Escherichia coli is the most common causative agent of urinary tract infections (UTIs) in 90-80% of patients in all age groups. Phylogenetic groups of these bacteria are variable and the most known groups are A, B1, B2 and D. The present study aimed to evaluate the phylogenetic groups of E. coli samples obtained from UTIs and their relation with antibiotic resistance patterns of isolates. Materials and Methods: In this study 113 E. coli isolates were isolated from distinct patients with UTIs referred to Hamadan hospitals. After biochemical and molecular identification of the isolates, typing and phylogenetic grouping of E. coli strains were performed using multiplex PCR targeting chu, yjaA and TSPE4.C2 genes. The anti-microbial susceptibility of the isolates to amikacin, ampicillin, trimethoprim-sulfamethoxazole, amoxicillin/clavulanic acid, ciprofloxacin, cefotaxime, imipenem, aztreonam, gentamicin, meropenem, nitrofurantoin, nalidixic acid and cefazolin was determined using disk diffusion method. Results: Of 113 isolates, 50 (44.2%), 35 (31%), 23 (20.4%) and 5 (4.4%) of samples belonged to group B2, group D, group A and group B1 phylogenetic groups respectively. All isolates were susceptible to meropenem, imipenem (100%), followed by amikacin (99.1%). The highest resistance rates were observed against ampicillin (74.3%) and nalidixic acid (70.8%). Correlation between phylogenetic groups and antibiotic susceptibilities was significant only with co-amoxiclav (P = 0.006), which had the highest resistance in phylogenetic group A. Conclusion: Prevalence of different phylogroup and resistance associated with them in E. coli samples could be variable in each region. Therefore, investigating of these items in E. coli infections, could be more helpful in selecting the appropriate antibiotic treatment and epidemiological studies.

Genomic insights into virulence, antimicrobial resistance, and adaptation acumen of Escherichia coli isolated from an urban environment

“…The present findings regarding the prevalence of isolates belonging to phylogroup B1 in the environmental samples are consistent with other findings ( 36 – 38 ). In addition to phylogroup B1, a reduced prevalence of isolates (E05 and ES11) belonging to the phylogroup E was also observed.…”

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

“…In the present research, the prevalence rate of ampicillin resistance in E. coli was the highest. The observed resistance pattern to ampicillin in E. coli ST131 clones in the current research was consistent with former studies in different provinces of Iran [45,46]. The highest prevalence of ampicillin resistance was found in isolates of patients ≤ 12 years old, and resistance rate to this antibiotic was low in the current study [47].…”

Phylogenetic Group Distribution of Uropathogenic Escherichia coli and Related Antimicrobial Resistance Pattern: A Meta-Analysis and Systematic Review

“…Based on our comprehensive search, 28 studies with eligibility criteria (Figure 1) were included in the meta-analysis (Ramos et al, 2011;Navidinia et al, 2013;Adib et al, 2014;Alizade et al, 2014a;Alizade et al, 2014b;Hemati et al, 2014;Kazemnia et al, 2014;Derakhshandeh et al, 2015;Iranpour et al, 2015;Rahdar et al, 2015;Salmani et al, 2016;Sohrabi and Zeighami, 2016;Hashemizadeh et al, 2017;Hojabri et al, 2017;Salehzadeh and Zamani, 2018;Bahadori et al, 2019;Farajzadah Sheikh et al, 2019;Norouzian et al, 2019;Shahin et al, 2019;Staji et al, 2019;Bakhtiari et al, 2020;Moez et al, 2020;Ranjbar et al, 2020;Yazdanpour et al, 2020). Of the 28 included studies, 17 and 9 studies reported the prevalence of phylogenic groups from hospitalized and community patients, respectively.…”