Antibiotic resistance pattern and pathological features of avian pathogenic Escherichia coli O78:K80 in chickens

Usman, S.; Anjum, A. A.; Usman, Muhammad; Imran, M. S.; Ali, M.; Moustafa, Mahmoud; Rehman,; Hussain, Taswar; Sarwar, F.; Azad, A.; Hussain, Irtaza; Naseer, Junaid; Tiwana, U.; Hafeez, None Saman

doi:10.1590/1519-6984.257179

Cited by 4 publications

(1 citation statement)

References 38 publications

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“…Terbentuknya resistensi baik terjadinya peningkatan maupun bertambahnya jenis antibiotik yang resisten akan menyebabkan terbentuknya strain yang resisten terhadap beberapa jenis antibiotika (multidrug-resistant) (Usmana et al, 2022), Multidrug-resistant yaitu menggambarkan isolat bakteri telah resisten terhadap beberapa jenis antibiotik (Magiorakos et al, 2012), setidaknya isolat bakteri telah resisten sedikit terhadap 3 jenis antibiotik (Eltai et al, 2018;Liu et al, 2020). ExPEC isolat asal burung puyuh dilaporkan telah terjadi multidrug-resistant (Mailafia et al, 2017).…”

Section: Hasil Dan Pembahasanunclassified

Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica)

Prihtiyantoro,

Purnomo,

et al. 2023

Jurnal Sain Veteriner

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Extraintestinal Escherichia coli (ExPEC) causes colibacillosis in poultry, including quail. ExPEC is a pathogenic E. coli that causes colibacillosis outside the gastrointestinal tract of poultry, in the form of peritonitis, pericarditis, salpingitis, synovitis, osteomyelitis, septicemia, pneumonitis, nephritis, pleurisy, proventriculitis and ventriculitis. In poultry farming antibiotics are used to promote growth and egg production, prevention and treatment of bacterial infections. Antibiotic resistance is a new problem that arises in poultry farming. In this study, 24 isolates of ExPEC strain from quail were used. These isolates did not differentiate sorbitol in the MacConkey (SMAC) sorbitol test assay. Antibiotic resistance was tested based on the inhibition of bacterial growth on Muller Hinnton Agar (MHA) media. 10 types of antibiotics were used to observe their resistance in all isolates. Resistance to Amikacin (87.5%), Ampicillin (87.5%), Ciprofloxacin (100%), Clindamycin (95.83%), Cefoxacin (25.0%), Doxycycline (87.5%), Erythromycin (100%), Gentamicin (95.83%), Penicillin (95.83%) and Tetracycline (83.3%). The isolates had multidrug-resistance between 3 to 10 types of antibiotics. This study showed that ExPEC isolates from quail were resistant to the type of antibiotics tested, so that the use of antibiotics in quail culture should be limited to reduce and prevent the emergence of new antibiotic resistance. Keyword: Escherichia coli, sorbitol-negative, quail, antibiotics resistance

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Section: Hasil Dan Pembahasanunclassified

Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica)

Prihtiyantoro,

Purnomo,

et al. 2023

Jurnal Sain Veteriner

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Potential of ZnO nanoparticles for multi-drug resistant Escherichia coli having CRISPR-Cas from poultry market in Lahore

Shabbir,

Shamim,

Tahir

et al. 2024

BMC Microbiol

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Background and objectives Apart from known factors such as irrational use of antibiotics and horizontal gene transfer, it is now reported that clustered regularly interspaced short palindromic repeats (CRISPR) are also associated with increased antimicrobial resistance. Hence, it is critical to explore alternatives to antibiotics to control economic losses. Therefore, the present study aimed to determine not only the association of CRISPR-Cas system with antibiotic resistance but also the potential of Zinc Oxide nanoparticles (ZnO-NPs) for avian pathogenic Escherichia coli (APEC) isolated from poultry market Lahore. Materials and methods Samples ( n = 100) were collected from live bird markets of Lahore, and isolates were confirmed as Escherichia coli ( E. coli ) using the Remel One fast kit, and APEC was identified using PCR. The antibiotic resistance pattern in APEC was determined using the minimum inhibitory concentration (MIC), followed by genotypic confirmation of antibiotic-resistant genes using the PCR. The CRISPR-Cas system was also identified in multidrug-resistant (MDR) isolates, and its association with antibiotics was determined using qRT-PCR. The potential of ZnO-NPs was evaluated for multidrug-resistant (MDR) isolates by MIC. Results All isolates of APEC were resistant to nalidixic acid, whereas 95% were resistant to chloramphenicol and 89% were resistant to streptomycin. Nineteen MDR APEC were found in the present study and the CRISPR-Cas system was detected in all of these MDR isolates. In addition, an increased expression of CRISPR-related genes was observed in the standard strain and MDR isolates of APEC. ZnO-NPs inhibited the growth of resistant isolates. Conclusions The findings showed the presence of the CRISPR-Cas system in MDR strains of APEC, along with the potential of ZnO-NPs for a possible solution to proceed. This highlights the importance of regulating antimicrobial resistance in poultry to reduce potential health consequences.

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