Antibiotic resistance pattern and frequency of cagA and vacA genes in Helicobacter pylori strains isolated from patients in Tabriz city, Iran

Rasi-Bonab, Farnaz; Jafari-Sales, Abolfazl; Shaverdi, Mohammad Amin; Navidifar, Tahereh; Saki, Morteza; Ghorbani, Atosa; Adekanmbi, Abimbola Olumide; Jafari, Behboud; Naebi, Sara

doi:10.1186/s13104-021-05633-5

Cited by 7 publications

(4 citation statements)

References 39 publications

(51 reference statements)

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“…Rasi Bonab et al, (2021) studied 221 samples of H. pylori isolated from patients. The frequency of cagA and vacA genes in patients was 63.5% to 81%, respectively [11], confirming the cases described in this study. The major pathophysiological event in H. pylori infection is the development and persistence of the inflammatory response due to these bacteria or their products [40].…”

Section: Discussionsupporting

confidence: 90%

The Role of Helicobacter Pylori Virulence Factors in Gastric Cancer

Ganjali¹,

fakheri²,

Bahari³

et al. 2022

Multidiscip Cancer Investig

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Introduction: Gastrointestinal (GI) cancers are considered among the most important causes of mortality and morbidity. Helicobacter pylori infection has been proven to be highly associated with the development of a variety of gastric diseases such as chronic gastritis, peptic ulcer disease (PUD), mucosa-associated lymphoid tissue (MALT), and gastric cancer (GC). To date, the exact role of the virulence factors in gastric diseases and other diseases remains elusive and controversial. Methods: The present study is a classic systematic review (expert opinion), in which articles published in English and Persian languages derived from Web of Science, Scopus, PubMed, and Iranian databases, including Magiran, IranMede, and scientific information database (SID) without any time limitation were explored using standardized keywords of H. pylori, virulence factors, gastric cancer, a combination of the above words, and other synonymous keywords. Finally, the information and obtained results were collected and interpreted. Results: In total, 14 of the 172 articles reviewed had inclusion criteria with the approval of the responsible author. According to the results, the development of chronic bacterial inflammation due to pathogenic mechanisms and factors, especially the role of cagA and vacA genes in gastric cancer, remains an important medical problem. Conclusions: Each of the H. pylori virulence factors can have a role in cancer development, and it appears that on-time H. pylori treatment is one of the best methods to prevent gastric cancer. Therefore, targeting Pathogenic factors of H. pylori to induce apoptosis and stimulate the immune system will be a promising, attractive, and helpful method for cancer prevention.

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Section: Discussionsupporting

confidence: 90%

The Role of Helicobacter Pylori Virulence Factors in Gastric Cancer

Ganjali¹,

fakheri²,

Bahari³

et al. 2022

Multidiscip Cancer Investig

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“…Due to increasing drug resistance of H . pylori to most common therapies, it is essential to set up a rapid and valid test for screening of antibiotic resistance genes in this bacterium [ 38 ]. The diagnosis of H .…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the clinical use of PCR-based testing is reduced by some factors like high costs, but the high diagnostic performance in the pre-and post-treatment setting, with the additional option of identifying clarithromycin-resistant strains, render it an excellent recent and future diagnostic technique [6]. Due to increasing drug resistance of H. pylori to most common therapies, it is essential to set up a rapid and valid test for screening of antibiotic resistance genes in this bacterium [38]. The diagnosis of H. pylori should be carried out in light of the clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter Pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: A validation study

2021

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There is still no agreement on the gold standard technique for diagnosing of H. Pylori in Iraq, as well as a paucity of data on the validity of different diagnostic techniques. This study aimed to investigate the prevalence of this bacterium with different methods and compare them to the quantitative polymerase chain reaction (qPCR) as a golden standard technique among Iraqi patients. In total, 115 Iraqi patients strongly suspected of H. pylori infection were enrolled in the current study. Invasive techniques including rapid urease testing (RUT) and gastric tissue culture in addition to non-invasive techniques including 14C-Urea breath test (14C-UBT), stool antigen test (SAT), CagA-IgG serology, and qPCR were performed to confirm the H. pylori infection. The qPCR was used as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. Overall, the prevalence rate of H. pylori in Iraqi patients was ranged from 47.8 to 70.4% using different methods. The positive results for each test were as follows: qPCR 81, (70.4%) UBT 79 (68.7%), SAT 77 (67%), RUT 76 (66.1%), Cag-IgG 61 (53%), and culture 55 (47.8%). The 14C-UBT showed the highest overall performance with 97.5% sensitivity, 97% specificity, and total accuracy of 97.3% followed by SAT, RUT, Cag-IgG, and culture method. Based on the accuracy of the studied methods for H. pylori detection, they can be arranged in order as follows: qPCR > UBT > SAT > RUT> CagA IgG > culture. Since a universal gold standard assay for the diagnosis of H. pylori has not yet been established in Iraq, the UBT may be recommended as first choice due to its higher performance compared to other methods.

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“…У когортному дослідженні серед болгарських пацієнтів виявлено збільшення точкових мутацій A2143G у домені V 23S рРНК серед менш вірулентних штамів VacA i2 та збільшення точкових мутацій A2142G у вірулентніших штамах VacA i1 [10]. Мутація A2143G прогнозує невдачу потрійної схеми ерадикації з кларитроміцином [15]. У нашому дослідженні точкові мутації A2143G і T2182C у домені V 23S рРНК виявлено у 26,3 % пацієнтів із загальної кількості досліджуваних.…”

Section: ефективність ерадикаційної терапіїunclassified

The role of highly toxigenic CagA and VacA strains of Helicobacter pylori in gastric carcinogenesis. Review

Nikulina¹,

Solovyova²

2022

СГ

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Gastric cancer takes the 4th place as the main cause of death among oncological diseases in the world. The risk factors of gastric cancer include genetic predisposition, alcohol abuse, smoking, low socio‑economic level, nutritional characteristics, Epstein–Barr virus and Helicobacter pylori infection. Helicobacter pylori bacterium is the only statistically proven risk factor for stomach cancer. Gastric cancer develops in less than 3 % of H. pylori ‑infected patients, suggesting a unique interaction between the host immune response and H. pylori virulence factors. Expression of oncogenic cytotoxin‑associated protein gene A (CagA), vacuolating cytotoxin A (VacA), and outer membrane proteins is important for H. pylori in host pathogenesis. The paper gives consideration for the relationship between the virulence factors of H. pylori and the host, which is necessary for a better understanding of the pathogenesis and prevention of carcinogenesis. The strategy for the prevention of gastric cancer includes eradication therapy, but it is complicated by the growth of resistance to antimicrobial drugs. Only insignificant number of papers investigate effects of pathogenicity factors of H. pylori (CagA+ and VacA+) on the development of atrophy, intestinal metaplasia and dysplasia. In the literature, there is little data on the difference in antibiotic resistance between H. pylori highly toxigenic and non‑toxigenic strains. The aim of the article is to present current data on the impact of highly toxigenic CagA+VacA+ strains of H. pylori on the processes of gastric carcinogenesis and to determine their role in resistance to clarithromycin. Investigation of the relationship between highly toxigenic CagA and VacA strains with clinical and morphological manifestations of gastritis and antibiotic resistance will enable preventing the progression of precancerous changes and using effective treatment tactics.

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Antibiotic resistance pattern and frequency of cagA and vacA genes in Helicobacter pylori strains isolated from patients in Tabriz city, Iran

Cited by 7 publications

References 39 publications

The Role of Helicobacter Pylori Virulence Factors in Gastric Cancer

The Role of Helicobacter Pylori Virulence Factors in Gastric Cancer

Detection of Helicobacter Pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: A validation study

The role of highly toxigenic CagA and VacA strains of Helicobacter pylori in gastric carcinogenesis. Review

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