Antibiotic Resistance Pattern among Different Species of Staphylococcus spp. with Emphasis on Detection of mecA Gene in Staphylococcus aureus Isolated from Patients in Tehran

Rahimi, Forough; Vandyousefi, J; Bouzari, Majid; Maleki, Zahra; Davoudi, Setareh

doi:10.1016/j.ijid.2008.05.729

Cited by 22 publications

(30 citation statements)

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“…This frequency rate was lower than other MRSA prevalence reports in Iran (16)(17)(18). It might be due to higher number of outpatient samples in comparison to inpatient samples in this hospital.…”

Section: Discussioncontrasting

confidence: 71%

Prophage Typing of Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Tehran, Iran

Rahimi

Bouzari

Katouli

et al. 2012

Jundishapur J Microbiol

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This study has highlighted the presence of bacteriophage types among MRSA isolates to show the high potential of these isolates to produce broad spectrum of virulence factors. Background:Staphylococcus aureus is a common cause of infections among humans and animals and it is known as a community-acquired and nosocomial pathogen. Most of the isolates contain lysogenic phages which are responsible for production of various virulence factors such as enterotoxins, staphylokinase, β-lysin, lipase, exfoliative toxin A and Pantone-vlaentine leukociden (PVL). All staphylococcus isolates are classified in 6 groups according to their sensitivity to 27 known lysogenic phages. Objectives: This study was performed to detect the presence of bacteriophage types and determine antibiotic resistance pattern of methicillin resistant S. aureus (MRSA) isolates obtained from a tertiary care hospital in Tehran, Iran from 2008 to 2010. Materials and Methods: A total of 968 S. aureus isolates were collected from a tertiary care hospital in Tehran, Iran and identified at the species level by PCR and biochemical tests. Susceptibility to 17 antibiotics was determined. Then oxacillin and vancomycin minimum inhibitory concentration (MIC) of the resistant isolates were determined. Multiplex-PCR was used to detect 6 classes of prophages. Results: Out of the 968 isolates 247 isolates were resistant to methicillin. Highest antibiotic resistance was seen to penicillin (100%), erythromycin (89.8%), kanamycin (89.4%), ciprofloxacin (88.6%) and tobramycin (87.4) respectively. None of the MRSA isolates showed resistance to vancomycin, synercid and linezolid. MIC results indicated that 46.1 and 4.4% of isolates with high (MIC ≥ 128 µg/ml) and low level (MIC ≥ 4 µg/ml) showed resistance to oxacillin, respectively. Four different phage types and eight patterns of prophages were detected. All MRSA isolates contained at least one prophage. Totally, 2.8, 69.2 and 27.9% contained 5, 4 and 3 different prophage types, respectively. Conclusions: High prevalence of different classes of prophages indicating the potential to carry a broad spectrum of virulence factors and high oxacillin resistance were found in the MRSA isolates. Detection of SGF phage in 100% of the isolates indicates the ability of these isolates to produce virulence factors.

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Section: Discussioncontrasting

confidence: 71%

Prophage Typing of Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Tehran, Iran

Rahimi

Bouzari

Katouli

et al. 2012

Jundishapur J Microbiol

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“…Also the study by Imani Fouladi et al (22), 75% of Staphylococcus aureus isolates with the SEB gene were Methicillin resistant and 15% were MSSA. In the study of Rahimi et al (23), all the isolates were susceptible to vancomycin and most were susceptible to SXT. MRSA isolates harbored all icaADBC genes, suggesting that these isolates are strong biofilm producers and considered to cause chronic and persistent infections (24).…”

Section: Discussionmentioning

confidence: 92%

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

Ghasemian

Peerayeh

Bakhshi

et al. 2015

Arch Pediatr Infect Dis

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Background:The icaABCD genes encode a Polysaccharide Intercellular Adhesion (PIA), which is a tight structure protecting Staphylococcus aureus community against adverse environmental conditions. The ica dependent biofilm formation plays an important role in persistent infections in hospitalized patients. Objectives: The aim of this study was to detect icaADBC genes encoding PIA among S. aureus isolates from children in Loghman Hospital of Tehran. Materials and Methods: We collected 22 clinical specimens from hospitalized pediatrics and identified the isolates. Then, we detected mecA gene among Methicillin Resistant S. aureus (MRSA), SCCmec types and icaABCD gens by PCR assay and specific primers. Results: Five isolates (22.7%) were methicillin resistant (MRSA) and mecA gene was detected among them. All the MRSA isolates harbored SCCmec type III. Prevalence of icaA, icaB, icaC and icaD in the isolates were 16 (73%), 14 (63.6%), 16 (73%) and 16 (73%), respectively. Moreover, all the MRSA strains were icaADBC positive. Conclusions: Prevalence of icaADBC genes was relatively high among children and also all the four ica genes were detected among MRSA strains.

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“…Staph aureus isolates were screened for methicillin resistance by disc diffusion method using oxacillin (1µg) and cefoxitin (30µg) disc and by determination of minimum inhibitory concentration (MIC) of oxacillin by agar dilution method per recommendation of CLSI method 10 . The discrepancies of the result were confirmed by PCR assay which is gold standard method for detection of mecA gene by using specific primers 27,28 . All MRSA isolates were tested for susceptibility against ceftriaxone (30µg), ciprofloxacin (5µg), doxycycline (30µg), erythromycin (15µg), gentamycin (10µg), rifampicin (5μg), vancomycin (30µg), fusidic acid (10µg) and linezolid (30µg) by disc diffusion method as recommended by CLSI 10 .…”

Section: Methodsmentioning

confidence: 91%

Emergence of Vancomycin Resistant Staphylococcus aureus during Hospital Admission at a Tertiary Care Hospital in Bangladesh

Khanam¹,

Haq

Shamsuzzaman

et al. 2017

Bangladesh J. Infect. Dis.

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Background: Glycopeptides such as vancomycin are frequently the choice of antibiotics for the treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA). For the last 7 years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus (VISA and VRSA respectively) has been increasing in various parts of the world. Objective: The present study was carried out to find out the presence of VISA and VRSA among isolated MRSA strains. Methodology: This cross sectional study was carried out in the Department of Microbiology in Dhaka medical college during period of January 2010 to December 2011. All S. aureus isolates were screened to detect methicillin resistance and then all MRSA isolates were subjected for MIC testing against vancomycin and oxacillin by agar dilution method, disc diffusion testing and PCR for mecA and pvl genes detection. Result: A total 112 S. aureus were isolated from 500 nasal swab sample collected from adult patients who were admitted in various departments and wards in Dhaka Medical College Hospital. Among 38 MRSA strains out of 112 Staph aureus isolates 3(7.89%) strains were resistance to vancomycin of which 2(5.26%) strains had MIC > 256 g/mL and one strain had MIC 256g/mL. All vancomycin resistance strains had MIC of oxacillin > 256 g/mL. All isolates possess mec-A gene. Conclusion: The present study reveals that emergence of VRSA upon admission at a tertiary care of hospital in Bangladesh. Continuous efforts should be made to prevent the spread and the emergence of VRSA by early detection of the resistant strains and using the proper infection control measures in the hospital setting.

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Antibiotic Resistance Pattern among Different Species of Staphylococcus spp. with Emphasis on Detection of mecA Gene in Staphylococcus aureus Isolated from Patients in Tehran

Cited by 22 publications

References 0 publications

Prophage Typing of Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Tehran, Iran

Prophage Typing of Methicillin Resistant Staphylococcus aureus Isolated from a Tertiary Care Hospital in Tehran, Iran

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

Emergence of Vancomycin Resistant Staphylococcus aureus during Hospital Admission at a Tertiary Care Hospital in Bangladesh

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