Antibiotic resistance of potential probiotic bacteria of the genus Lactobacillus from human gastrointestinal microbiome

Ботина, С. Г.; Полуэктова, Е. У.; Glazova, A. A.; Zakharevich, Natalia V.; Koroban, N. V.; Зинченко, В.В.; Babykin, M. M.; Zhilenkova, O. G.; Amerkhanova, A. M.; Даниленко, В. Н.

doi:10.1134/s0026261711020032

Cited by 16 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…None of the infants in the present study had used any antibiotics and most strains were sensitive to ampicillin, imipenem, erythromycin and gentamycin. These results are consistent with previous reports . All L. rhamnosus, L. paracasei and L. fermentum strains had a high percentage of intrinsic resistance to vancomycin, which is in accordance with the finding of Delgado et al .…”

Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

“…Because it is important that probiotic bacteria are of human origin , recent studies have evaluated probiotic potential of Lactobacillus strains isolated from humans . Most lactobacilli isolated from infants are reportedly sensitive to antibiotics . Also, some probiotic properties such as gut survival, adhesion to epithelium cells and inhibition of pathogen bacteria have been identified in lactobacilli isolated from humans .…”

mentioning

confidence: 99%

“…Most lactobacilli isolated from infants are reportedly sensitive to antibiotics . Also, some probiotic properties such as gut survival, adhesion to epithelium cells and inhibition of pathogen bacteria have been identified in lactobacilli isolated from humans . Review of published reports yielded little information about Lactobacillus species isolated from infant feces in Iran .…”

mentioning

confidence: 99%

See 3 more Smart Citations

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Halimi

Mirsalehian

2016

Microbiology and Immunology

View full text Add to dashboard Cite

The probiotic potential of Lactobacillus species isolated from infant feces was investigated. For this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (Caco-2 cells) of four commonwere assessed. Most isolates that which were sensitive to imipenem, ampicillin, gentamycin, erythromycin and tetracycline were selected for other tests. L. gasseri isolates had the greatest sensitivity to gastric and intestinal fluids (<10% viability). L. fermentum (FH5, FH13 and FH18) had the highest adhesion to Caco-2 cells. The lowest antibacterial activity against pathogenic bacteria was shown by L. gasseri strains in spot tests. Furthermore, non-adjusted cell-free culture supernatants with low pH had greater antimicrobial activity, which was related to organic acid. The results showed that some isolates of L. rhamnosus and L. fermentum are suitable for use as a probiotic.

show abstract

Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Halimi

Mirsalehian

2016

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…Moreover, our study found that 70% of the tested Lactobacillus strains were resistant to ciprofloxacin, which is similar to a study conducted by Li and others (). Thirteen Lactobacillus strains showed different degree of sensitivity to ampicillin, chloramphenicol, rifampicin, roxithromycin, erythromycin, and azithromycin, whereas none of them were sensitive to all tested antibiotics (Botina and others ). Hummel and others () investigated the antibiotic resistance profiles of 45 LAB strains, and they found that aminoglycoside (gentamicin and streptomycin) and ciprofloxacin resistance was higher than 70%, whereas only a low resistance level was observed for ampicillin, penicillin, chloramphenicol, and tetracycline.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Antibiotic Resistance Genes from Lactobacillus Isolated from Traditional Dairy Products

Guo

Pan

et al. 2017

Journal of Food Science

127

View full text Add to dashboard Cite

Lactobacilli are widely used as starter cultures or probiotics in yoghurt, cheese, beer, wine, pickles, preserved food, and silage. They are generally recognized as safe (GRAS). However, recent studies have shown that some lactic acid bacteria (LAB) strains carry antibiotic resistance genes and are resistant to antibiotics. Some of them may even transfer their intrinsic antibiotic resistance genes to other LAB or pathogens via horizontal gene transfer, thus threatening human health. A total of 33 Lactobacillus strains was isolated from fermented milk collected from different areas of China. We analyzed (1) their levels of antibiotic resistance using a standardized dilution method, (2) their antibiotic resistance gene profiles by polymerase chain reaction (PCR) using gene-specific primers, and (3) the transferability of some of the detected resistance markers by a filter mating assay. All Lactobacillus strains were found to be resistant to vancomycin, but susceptible to gentamicin, linezolid, neomycin, erythromycin, and clindamycin. Their susceptibilities to tetracycline, kanamycin, ciprofloxacin, streptomycin, quinupristin/dalfopristin, trimethoprim, ampicillin, rifampicin, and chloramphenicol was different. Results from our PCR analysis revealed 19 vancomycin, 10 ciprofloxacin, and 1 tetracycline-resistant bacteria that carried the van(X), van(E), gyr(A), and tet(M) genes, respectively. Finally, no transferal of the monitored antibiotic resistance genes was observed in the filter mating assay. Taken together, our study generated the antibiotic resistance profiles of some milk-originated lactobacilli isolates and preliminarily assessed their risk of transferring antibiotic gene to other bacteria. The study may provide important data concerning the safe use of LAB.

show abstract

Determination and Safety Aspects of Probiotic Cultures

Patra¹,

Duary²

2022

Probiotics, Prebiotics and Synbiotics

View full text Add to dashboard Cite

Antibiotic resistance of potential probiotic bacteria of the genus Lactobacillus from human gastrointestinal microbiome

Cited by 16 publications

References 19 publications

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Assessment and comparison of probiotic potential of four Lactobacillus species isolated from feces samples of Iranian infants

Characterization of Antibiotic Resistance Genes from Lactobacillus Isolated from Traditional Dairy Products

Determination and Safety Aspects of Probiotic Cultures

Contact Info

Product

Resources

About