Antibiotic Resistance of<i>Listeria monocytogenes</i>Isolated from Meat-Processing Environments, Beef Products, and Clinical Cases in Brazil

Camargo, Anderson Carlos; Castilho, Natália Parma Augusto de; Silva, Danilo Augusto Lopes da; Vallim, Deyse Christina; Hofer, Ernesto; Nero, Luís Augusto

doi:10.1089/mdr.2014.0270

Cited by 29 publications

(17 citation statements)

References 33 publications

(52 reference statements)

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“…30 have also found Salmonella typhimurium isolates from Indian foods to be sensitive to ampicillin and ciprofloxacin. Our data showed that all isolates of Listeria except P4 showed resistance to one or more of the tested antimicrobial drugs, this observation is consistent with previous studies such as those of Rahimi et al (2012) 31 ; Carmago et al (2015) 32 and Wieczorek et al (2012) 33 . These authors reported significant levels of antimicrobial resistant Listeria isolates from meat and hides circulating in Iran, Brazil and Poland respectively.…”

Section: Discussion: Food Borne Disease Caused Bysupporting

confidence: 93%

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2016

IJPSR

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Background: Widespread use of antibiotics in livestock production in large-scale across the globe has become of public and veterinary health importance because of its implication in antibiotic resistance. Adequate data in this area of research is not readily available in Nigeria; this study was undertaken in view of the possible link between antimicrobial resistance in farm animals and humans. Methods: We collected fifty samples of raw beef from different vendors and slaughter houses within Abuja and screened them for the presence of Listeria monocytogenes and Salmonella typhimurium using standard microbiological methods. The total bacterial and fungal counts, susceptibility of the isolates to different antibiotics and heat sensitivity at 55, 60 and 65ºC for 15 minutes were determined. Results: Our results show that ten isolates of Listeria monocytogenes and eighteen isolates of Salmonella typhimurium were isolated from the samples. The total viable bacteria count range was 1x 10 9 -8x 10 9 cfu/g while the fungal count was 1x 10 3 -9x 10 9 cfu/g. One (10 %) of the Listeria monocytogenes isolates was resistant to all antibiotics tested while all the Listeria monocytogenes isolates were resistant to cefuroxime. Eight (44.4%) of the Salmonella typhimurium isolates were resistant to at least three antibiotics. All the Listeria monocytogenes and Salmonella typhimurium isolates did not survive beyond 60 o C upon heat treatment. Conclusions: Our results indicate high prevalence of Salmonella typhimurium and Listeria monocytogenes in selected beef in Abuja. Beef therefore may represent a large reservoir for antimicrobial-resistant Salmonella typhimurium and Listeria monocytogenes.

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Section: Discussion: Food Borne Disease Caused Bysupporting

confidence: 93%

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2016

IJPSR

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“…In our study, resistance to oxacillin was 24% for L. monocytogenes isolated from selected sources. Oxacillin resistance has been already associated to L. monocytogenes with even higher percentages in research studies from Brazil, United States, and Poland (Davis and Jackson, 2009;Wieczorek et al, 2012;Camargo et al, 2015). In Staphylococcus infections, oxacillin susceptibility test is used as a marker for methicillin-resistant Staphylococcus aureus.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility and Multilocus Sequence Typing ofListeria monocytogenesIsolated Over 11 Years from Food, Humans, and the Environment in Italy

Caruso

Fraccalvieri

Pasquali

et al. 2020

Foodborne Pathogens and Disease

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Due to the increasing number of studies reporting the detection of antimicrobial-resistant isolates of Listeria monocytogenes, we sought to determine the antimicrobial susceptibility of L. monocytogenes isolates collected in Italy and find potential correlations to their serotypes and multilocus sequence types (MLST). The antimicrobial susceptibility of 317 L. monocytogenes isolates collected from food, humans, and the environment from 1998 to 2009 was assessed by minimum inhibitory concentration (MIC). Serotyping and MLST was also performed on all isolates. Potential correlations among antimicrobial resistance profiles, serotyping, and MLST were statistically evaluated. Twenty-four percent of L. monocytogenes isolates were resistant to oxacillin, 28.7% intermediate to clindamycin, and 24.3% to ciprofloxacin. The majority of isolates with elevated MIC to oxacillin was of environmental origin and belonged to serotype 4b/4e and ST2. Isolates with intermediate MIC values to clindamycin and ciprofloxacin were mostly of food and human origin and belonged to serotype 4b/4e and ST9. Regarding the time frame of isolate collection, comparing the last 3 years (2007-2009) to previous years (1998-2006), an increase was observed in the percentage of resistant and intermediate isolates per year. This trend strongly suggests the need for increasing attention on the prevalence of antimicrobial resistance in L. monocytogenes in Italy. To predict future resistance trends, the monitoring of clinical intermediate resistance might represent a useful tool especially for antibiotics associated to multiple-step mechanisms of acquired resistance. A specific focus should be addressed to antimicrobial-resistant isolates of serotype 4b, repeatedly associated with food-borne outbreaks.

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“…Lm isolates selection A collection of Brazilian isolates, most of them kindly provided by FioCruz (CLIST collection), were previously characterized (Camargo et al, 2015;Camargo et al, 2016). Based on this work, a total of 35 representative Lm isolates were selected for WGS based on their year of isolation (from 1978 to 2013), serotypes (1/2a, n = 7; 1/2b, n = 7; 1/2c, n = 6; 4b, n = 15), sources (PE, n = 5; beef, n = 16; and clinical, n = 14) and geographic distribution in the country (from eight states).…”

Section: Methodsmentioning

confidence: 99%

Whole‐genome sequencing reveals Listeria monocytogenes diversity and allows identification of long‐term persistent strains in Brazil

Camargo

Moura

Avillan

et al. 2019

Environmental Microbiology

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Summary Advances in whole‐genome sequencing (WGS) technologies have documented genetic diversity and epidemiology of the major foodborne pathogen Listeria monocytogenes (Lm) in Europe and North America, but data concerning South America are scarce. Here, we examined the population structure and genetic diversity of this major foodborne pathogen collected in Brazil. Based on core genome multilocus sequence typing (cgMLST), isolates from lineages I (n = 22; 63%) and II (n = 13; 37%) were distributed into 10 different sublineages (SLs) and represented 31 new cgMLST types (CTs). The most prevalent SLs were SL9 (n = 9; 26%), SL3 (n = 6; 17%) and SL2 and SL218 (n = 5; 14%). Isolates belonging to CTs L2‐SL9‐ST9‐CT4420 and L1‐SL315‐ST520‐CT4429 were collected 3 and 9 years apart, respectively, revealing long‐term persistence of Lm in Brazil. Genetic elements associated with stress survival were present in 60% of isolates (57% SSI‐1 and 3% SSI‐2). Pathogenic islands were present in 100% (LIPI‐1), 43% (LIPI‐3) and 6% (LIPI‐4) of the isolates. Mutations leading to premature stop codons were detected in the prfA and inlA virulence genes. This study is an important contribution to understanding the genomic diversity and epidemiology of Lm in South America. In addition, the results highlight the importance of using WGS to reveal Lm long‐term persistence.

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Antibiotic Resistance ofListeria monocytogenesIsolated from Meat-Processing Environments, Beef Products, and Clinical Cases in Brazil

Cited by 29 publications

References 33 publications

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Antimicrobial Susceptibility and Multilocus Sequence Typing ofListeria monocytogenesIsolated Over 11 Years from Food, Humans, and the Environment in Italy

Whole‐genome sequencing reveals Listeria monocytogenes diversity and allows identification of long‐term persistent strains in Brazil

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