Antibiotic resistance of airborne bacterial populations in a hospital environment

Tamsi, Nur Sarah Fatihah; Latif, Mohd Talib; Othman, Murnira; Bakar, Farah Diba Abu; Yusof, Hanizah Mohd; Noraini, Nor Mohd Razif; Zahaba, Maryam; Sahani, Maneesh

doi:10.1007/s10661-022-10291-6

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…For years, a culture-dependent method would be relied upon to identify a microbial community in various settings, including the indoor air [11][12][13]. The collection of indoor air samples is a crucial step as it requires drawing a sample that represents the exposure of bacterial community which is present in the given location.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies conducted in healthcare settings in Malaysia have relied on culture-dependent method to determine the bacterial concentrations in indoor air. These studies also involved a series of biochemical analysis for characterizing and pro ling the bacterial community [12,27]. However, in the case of outbreaks, this method does not provide signi cant assistance due to the lengthy analysis period and inconclusive ndings, primarily because it excludes unculturable bacteria [28].…”

Section: Discussionmentioning

confidence: 99%

“…Studies on indoor air microbiomes may help to understand the potential causative agents that contribute to nosocomial infections. In the past, microbial communities in indoor air and water sources were often identi ed using a culture-dependent method [11][12][13], which involved collecting samples and culturing bacteria on agar plates using NMAM 0800 [14][15][16]. This method had limitations, including the inability to culture certain microbes and being timeconsuming.…”

Section: Introductionmentioning

confidence: 99%

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May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace

Hussin,

Tay,

Zainulabid

et al. 2024

Preprint

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The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further research on specific threats within the hospital environment. In this study, an independent culture-based approach was used to analyze the baseline core microbiome present in hospital environments, utilizing amplicon sequencing on the next-generation sequencing technology to target the V3 region of the 16S rRNA gene. Firmicutes, Proteobacteria, and Actinobacteria were the main bacterial phyla that were most isolated from the wards and clinics with different orders of abundance; Firmicutes being associated more in clinics and Actinobacteriota in wards. The bacteria Niallia taxi, Methyloversatilis universalis, unclassified Rummeliibacillus, unclassified Clostridium, and unclassified Sphingomonadaceae dominated the clinic area while ward areas reported Pseudonocardia bannensis, Rubrobacter A aplysinae, unclassified Brachybacterium, unclassified Bradyrhizobium, and unclassified Mycobacterium to be the top five features. While the alpha-diversity index showed no significant differences, the beta-diversity analysis showed a significant differences between clinic and ward areas (p < 0.05). Certain bacterial species associated with opportunistic pathogens as well as normal skin flora such as Methylobacterium spp., Cutibacterium spp., unclassified Sphingomonadaceae, and Anoxybacillus B spp., were also identified across all samples. The methods described in this research aim to establish a rapid and sensitive screening process that could be valuable for disease surveillance within the healthcare setting, shedding light on the potential impacts of the hospital microbiome on human illness.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%