Antibiotic resistance of Aeromonas ssp. strains isolated from Sparus aurata reared in Italian mariculture farms

Scarano, Christian; Piras, Francesca; Virdis, S.; Ziino, Graziella; Nuvoloni, Roberta; Dalmasso, Alessandra; Santis, E.P.L. De; Spanu, Carlo

doi:10.1016/j.ijfoodmicro.2018.07.033

Cited by 83 publications

(69 citation statements)

References 43 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Linkage between antibiotic use and resistance has been demonstrated for other aquaculture-associated bacteria (Verner-Jeffreys et al, 2009; Tamminen et al, 2010; Laganà et al, 2011; Scarano et al, 2014), and similar data has been reported in animal husbandry facilities. These findings have resulted in calls to ban non-medical use of antibiotics (Meek et al, 2015).…”

Section: Discussionsupporting

confidence: 62%

Evidence of Increased Antibiotic Resistance in Phylogenetically-Diverse Aeromonas Isolates from Semi-Intensive Fish Ponds Treated with Antibiotics

et al. 2016

View full text Add to dashboard Cite

The genus Aeromonas is ubiquitous in aquatic environments encompassing a broad range of fish and human pathogens. Aeromonas strains are known for their enhanced capacity to acquire and exchange antibiotic resistance genes and therefore, are frequently targeted as indicator bacteria for monitoring antimicrobial resistance in aquatic environments. This study evaluated temporal trends in Aeromonas diversity and antibiotic resistance in two adjacent semi-intensive aquaculture facilities to ascertain the effects of antibiotic treatment on antimicrobial resistance. In the first facility, sulfadiazine-trimethoprim was added prophylactically to fingerling stocks and water column-associated Aeromonas were monitored periodically over an 11-month fish fattening cycle to assess temporal dynamics in taxonomy and antibiotic resistance. In the second facility, Aeromonas were isolated from fish skin ulcers sampled over a 3-year period and from pond water samples to assess associations between pathogenic strains to those in the water column. A total of 1200 Aeromonas isolates were initially screened for sulfadiazine resistance and further screened against five additional antimicrobials. In both facilities, strong correlations were observed between sulfadiazine resistance and trimethoprim and tetracycline resistances, whereas correlations between sulfadiazine resistance and ceftriaxone, gentamicin, and chloramphenicol resistances were low. Multidrug resistant strains as well as sul1, tetA, and intI1 gene-harboring strains were significantly higher in profiles sampled during the fish cycle than those isolated prior to stocking and these genes were extremely abundant in the pathogenic strains. Five phylogenetically distinct Aeromonas clusters were identified using partial rpoD gene sequence analysis. Interestingly, prior to fingerling stocking the diversity of water column strains was high, and representatives from all five clusters were identified, including an A. salmonicida cluster that harbored all characterized fish skin ulcer samples. Subsequent to stocking, diversity was much lower and most water column isolates in both facilities segregated into an A. veronii-associated cluster. This study demonstrated a strong correlation between aquaculture, Aeromonas diversity and antibiotic resistance. It provides strong evidence for linkage between prophylactic and systemic use of antibiotics in aquaculture and the propagation of antibiotic resistance.

show abstract

Section: Discussionsupporting

confidence: 62%

Evidence of Increased Antibiotic Resistance in Phylogenetically-Diverse Aeromonas Isolates from Semi-Intensive Fish Ponds Treated with Antibiotics

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The classes of antibiotics used both for therapeutic and prophylaxis, on the basis of legal permissions in Italian aquaculture, include beta-lactams (amoxicillin, ampicillin), tetracyclines (clortetracycline, tetracycline, oxytetracycline), amphenicols (florfenicol, thiamphenicol), macrolides (erythromycin), sulfonamides (all, included trimethoprim+sulfonamide) and quinolones (oxolinic acid, flumequin, enrofloxacin) [62,63]. A review of the scientific literature on AMR in Italian aquaculture has evidenced a common resistance to beta-lactams, tetracycline, erythromycin and trimethoprim, and a general susceptibility to chloramphenicol, tobramycin and flumequin [64]. Of interest, multiple antibiotic resistance profiles have been reported for Aeromonas and Vibrio [65][66][67][68].…”

Section: Discussionmentioning

confidence: 99%

“…A review of the scientific literature on AMR in Italian aquaculture has evidenced a common resistance to beta-lactams, tetracycline, erythromycin and trimethoprim, and a general susceptibility to chloramphenicol, tobramycin and flumequin [64]. Of interest, multiple antibiotic resistance profiles have been reported for Aeromonas and Vibrio [65][66][67][68].…”

Section: Discussionmentioning

confidence: 99%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

View full text Add to dashboard Cite

Antimicrobial resistance is a major public health concern restricted not only to healthcare settings but also to veterinary and environmental ones. In this study, we analyzed, by whole genome sequencing (WGS) the resistome, mobilome and virulome of 12 multidrug-resistant (MDR) marine strains belonging to Shewanellaceae and Vibrionaceae families collected at aquaculture centers in Italy. The results evidenced the presence of several resistance mechanisms including enzyme and efflux pump systems conferring resistance to beta-lactams, quinolones, tetracyclines, macrolides, polymyxins, chloramphenicol, fosfomycin, erythromycin, detergents and heavy metals. Mobilome analysis did not find circular elements but class I integrons, integrative and conjugative element (ICE) associated modules, prophages and different insertion sequence (IS) family transposases. These mobile genetic elements (MGEs) are usually present in other aquatic bacteria but also in Enterobacteriaceae suggesting their transferability among autochthonous and allochthonous bacteria of the resilient microbiota. Regarding the presence of virulence factors, hemolytic activity was detected both in the Shewanella algae and in Vibrio spp. strains. To conclude, these data indicate the role as a reservoir of resistance and virulence genes in the environment of the aquatic microbiota present in the examined Italian fish farms that potentially might be transferred to bacteria of medical interest.

show abstract

“…Vibrio species are autochthonous inhabitants of marine and estuarine environments and are typically isolated from marine organisms (3) . Although some of them are recognized as pathogenic species such as V. cholerae, V. parahaemolyticus, or V. vulnificus, others are responsible for major economic losses in the aquaculture sector, such as V. anguillarum and V. harveyi that cause vibriosis (bacterial diseases affecting wild and farmed fish) with high rates of mortality (4,5) . Majority of Vibrio species are thought to have a bank for resistance genes in the aquaculture environment.…”

Section: Introductionmentioning

confidence: 99%

Estimation Of β-lactamase in Marine Water Isolates

Hanif¹,

Ansari²,

Badar³

et al. 2017

IAM

View full text Add to dashboard Cite

Objective: β-lactamase enzyme is encoded by the plasmid mediated genes which is very effective against β-lactam antibiotics. This enzyme is produced by many gram negative bacteria which consequences hinder in in therapy with many efficient β-lactam antibiotics. The aim of this study is to assess the susceptibility of the strain, isolated from marine water against Ampicillin (antibiotics) and the production of β-lactamase. Material and Methods: Marine water samples were collected from Clifton Beach during summer in sterile condition; organisms were identified and characterized through biochemical analysis. MIC for ampicillin was determined by broth dilution method. Plasmid extraction was performed by following the modified method of Birnboim Doly. β-lactamase was also detected by iodometric cell suspension method. Results: Ampicillin resistance was observed in selected strain. Isolated gram negative bacilli strain was highly resistant to ampicillin (≥ 2500µg/ml). Through the analysis of colonial morphology, microscopic examination and plasmid extraction (which was found as 10 kb in size) strain was suspected as Vibrio spp. The results of iodometric assay for β-lactamase was also considerable. Conclusion: The dreadful spread of antibiotic resistant mechanism in aquatic environment and the excessive use of antibiotic were highlighted. Marine isolates were characterized and identified as luminescent bacteria. A simple and convenient method of β-lactamase analysis through iodometric assay has been applied which was found to be a reliable technique as compare to other β-lactamase quantification method.

show abstract

Antibiotic resistance of Aeromonas ssp. strains isolated from Sparus aurata reared in Italian mariculture farms

Cited by 83 publications

References 43 publications

Evidence of Increased Antibiotic Resistance in Phylogenetically-Diverse Aeromonas Isolates from Semi-Intensive Fish Ponds Treated with Antibiotics

Evidence of Increased Antibiotic Resistance in Phylogenetically-Diverse Aeromonas Isolates from Semi-Intensive Fish Ponds Treated with Antibiotics

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Estimation Of β-lactamase in Marine Water Isolates

Contact Info

Product

Resources

About