Antibiotic resistance mechanisms in Acinetobacter spp. strains isolated from patients in a paediatric hospital in Mexico

Bello-López, Elena; Rocha-Gracia, Rosa del Carmen; Castro-Jaimes, Semiramis; Cevallos, Miguel A.; Vargas-Cruz, Michelle; Verdugo-Yocupicio, Ricardo; Sáenz, Yolanda; Torres, Carmén; Gutiérrez-Cazarez, Zita; Arenas-Hernández, Margarita María de la Paz; Lozano-Zaraín, Patricia

doi:10.1016/j.jgar.2020.08.014

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…Several Acinetobacter and Pseudomonas species were previously reported from different clinical samples from both animal, and human infections ( Chusri et al, 2014 ; Wong et al, 2017 ; Agnese et al, 2018 ). Many of them were resistant to multiple classes of antibiotics primarily by several intrinsic resistance mechanism they encode, and secondly by acquired resistance mechanisms ( Bello-López et al, 2020 ; Meng et al, 2020 ). Studies have also shown that multidrug-resistant strains of P. aeruginosa and A. baumannii were the main drivers of hospital-acquired infections ( Djahmi et al, 2014 ; Eichenberger and Thaden, 2019 ; Kazmierczak et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and antimicrobial susceptibility of Pseudomonas spp. and Acinetobacter spp. from clinical samples at Jimma medical center, Ethiopia

Sewunet

Asrat²,

Woldeamanuel³

et al. 2022

Front. Microbiol.

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IntroductionPseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii) can cause difficult-to-treat infections. We characterized molecular epidemiology of ceftazidime-resistant P. aeruginosa and carbapenem-resistant A. baumannii at a tertiary hospital in Ethiopia.Materials and methodsNon-fermenting gram-negative bacilli (n = 80) isolated from admitted patients were subjected for species identification by MALDI-TOF. Pseudomonas species resistant to ceftazidime or meropenem, and Acinetobacter species resistant to meropenem, or imipenem were selected for whole genome sequencing. DNA extracted with EZ1 Advanced XL instrument (Qiagen, Hilden, Germany) was sequenced on Illumina (HiSeq2500) using libraries prepared by NEXTRA-kits (Illumina). Raw reads were assembled using SPAdes 3.13.0, and assembled genomes were used to query databases for resistome profile and sequence types.ResultAmong Pseudomonas species isolated, 31.7% (13/41), and 7.3% (3/41) were non-susceptible to ceftazidime, and meropenem, respectively. Carbapenem-resistance was 56.4% (22/39) among Acinetobacter species. Moreover, 92% (12/13) of Pseudomonas species non-susceptible to ceftazidime and/or meropenem, and 89.4% (17/19) of Acinetobacter species encoded multiple resistance genes for at least three classes of antimicrobials. The prevalent β - lactamase genes were blaOXA–486 (53.8%, 7/13), blaCTX–M–15 (23.0%, 3/13) among Pseudomonas, and blaGES–11 (57.8%, 11/19) among Acinetobacter. The blaOXA–51-like β - lactamase, blaOXA–69 (63.1%, 12/19) was the most prevalent carbapenemase gene among Acinetobacter isolates. Single isolates from both P. aeruginosa, and A. baumannii were detected with the blaNDM–1. Sequence type (ST)1 A. baumannii and ST274 P. aeruginosa were the prevalent sequence types. A cgMLST analysis of the ST1 A. baumannii isolates showed that they were closely related and belonged to the international clonal complex one (ICC1). Similarly, ST274 P. aeruginosa isolates were clonally related.ConclusionThe prevalence of MDR isolates of Pseudomonas and Acinetobacter spp. was high. A. baumannii isolates were clonally spreading in the admission wards at the hospital. Emergence of blaNDM–1 in the intensive care, and surgical wards of the hospital is a severe threat that requires urgent intervention.

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Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and antimicrobial susceptibility of Pseudomonas spp. and Acinetobacter spp. from clinical samples at Jimma medical center, Ethiopia

Sewunet

Asrat²,

Woldeamanuel³

et al. 2022

Front. Microbiol.

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“…baumannii is one of the main causative agents of healthcare-associated infections worldwide [10]. Currently, the information regarding the clinical and molecular characterization of carbapenem-resistant A. baumannii infections in the pediatric population is scarce [11,12]. A high prevalence of the neonatal population (44.8%) was described in our study, with ventilator-associated pneumonia being most frequent type of infection (48.9%), and a high rate of MDR-resistant isolates (48.9%).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and drug susceptibility of Acinetobacter baumannii infection in children from a third-level hospital in Mexico

Bejarano

Díaz

Cavazos

et al. 2023

Preprint

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Objectives Acinetobacter spp. has become a threat public health threat due to the high prevalence of multidrug-resistant strains. There is limited information on the clinical and molecular features of carbapenem-resistant A. baumannii (CRAB) infections in children. Our study aimed to describe the clinical and molecular characteristics of CRAB infections among pediatric patients at a tertiary center in Mexico. Methods Consecutive cases of CRAB infections were recorded between 2017–2022. Clinical and demographic data were collected from clinical records. Identification of the strains was carried out through mass spectrometry. Confirmation of A. baumannii strains was done using a PCR method based on gyrB sequence. Additionally, PCR was used to detect the presence of carbapenemase-encoding resistance genes. Results 49 CRAB infections were reported, 69.3% (n = 34) female. 44.8% (n = 22) were neonates. The median hospital length of stay was 32 days (interquartile range [IQR], 19–60). 48.9% of the isolates were recovered from bronchial secretions. Most of the antibiotic groups exhibited a co-resistance rate exceeding 40%. All carbapenem-resistant strains carried blaOXA−51, and blaOXA−24 genes. BlaIMP genes were detected in half of the cases, with all strains co-harboring blaOXA−24 genes. Conclusion The study showed a high prevalence of CRAB infections in neonates, along with a high prevalence of co-resistance to antibiotics, and a high rate of isolates carrying blaOXA-24, and blaIMP genes. CRAB poses a major threat due to the mortality rate and limited therapeutic options, it is imperative to urgently halt the propagation of carbapenem-resistant Acinetobacter baumannii.

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“…These genera were represented by Acinetobacter genomospecies 3, Acinetobacter junii , Pseudomonas aeruginosa , and Pseudomonas amygdali ; these species have been isolated from hospital environments ( Horrevorts et al , 1995 ; Bassetti et al , 2021 ) and they have been associated with antibiotic resistance ( Bello-López et al , 2020 ; Panzuti et al , 2020 ; Gruszecka et al , 2021 ). In horses, Acinetobacter baumannii and Acinetobacter calcoaceticus have been isolated from a venous catheter-associated with thrombophlebitis ( Vaneechoutte et al , 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares

Thomson¹,

Pareja²,

ez³

et al. 2022

Open Vet J

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Background: Culture-independent techniques have made it possible to expand the knowledge about the composition of bacterial communities present in the healthy uterus and their role in health and disease, mainly in humans. However, in animals like mares, there is a dearth of information regarding this area. Aim: To narrow this knowledge gap, the objective of this study was to identify and characterize the composition and function for the uterine microbiome of a group of Chilean purebred mares (CPM), an equine breed with the oldest genealogical record in South America and an economical important reproductive industry. Methods: From uterine biopsy samples obtained during estrus, DNA extraction and targeted sequencing was performed to investigate the bacterial diversity and its probable metabolic function. Results: CPM biopsy samples were characterized by having a varied microbial composition, where the four most relatively abundant phyla were Proteobacteria (69.6%), Firmicutes (21.1%), Bacteroidetes (7.8%), and Actinobacteria (1.06%); which made up 99.6% of the total identified phyla. In contrast, Actinobacteria and Fusobacteria were the phyla not identified in all samples. Of a total of 59 genera identified across all samples, Staphylococcus was the most abundant genus with an average relative abundance of 18.88%, followed by Pseudomonas (17.9%), Escherichia/Shigella (10.42%), and Klebsiella (9.92%). Conclusion: These findings contribute to the knowledge of microbes presence in the uterus, while future studies are required to demonstrate the role of these microorganisms in health and disease.

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Antibiotic resistance mechanisms in Acinetobacter spp. strains isolated from patients in a paediatric hospital in Mexico

Cited by 14 publications

References 36 publications

Molecular epidemiology and antimicrobial susceptibility of Pseudomonas spp. and Acinetobacter spp. from clinical samples at Jimma medical center, Ethiopia

Molecular epidemiology and antimicrobial susceptibility of Pseudomonas spp. and Acinetobacter spp. from clinical samples at Jimma medical center, Ethiopia

Molecular characterization and drug susceptibility of Acinetobacter baumannii infection in children from a third-level hospital in Mexico

Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares

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