Antibiotic resistance in microorganisms isolated in a bull semen stud

Goularte, Karina Lemos; Voloski, Flávia Liége Schütz; Redü, J. F. M.; Ferreira, C. E. R.; Vieira, Arnaldo Diniz; Duval, Eduarda Hallal; Mondadori, Rafael Gianella; Lucia, Thomaz

doi:10.1111/rda.13621

Cited by 12 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although such high concentrations are commonly not present in the insemination straws, we suggest to target the antibacterial supplementation of insemination doses against the selected species. Especially, bacterial species resistant to frequently used antibiotics may represent a high risk [ 33 ]. For the future, it would be interesting to monitor intracellular Mg and Ca concentration in order to investigate if the environment with a reduced Mg and Ca concentration would be directly reflected in reduced Mg and Ca amounts inside the cell, and thus a lower availability of these cations for the metabolic processes.…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus-Induced Bacteriospermia In Vitro: Consequences on the Bovine Spermatozoa Quality, Extracellular Calcium and Magnesium Content

Ďuračka

Husarčíková

Jančov

et al. 2021

Animals

View full text Add to dashboard Cite

Bacterial contamination of bovine ejaculates intended for artificial insemination may be reflected in a significant economic loss due to unsuccessful fertilization as well as health issues of the recipients. The Staphylococcus genus represents a large part of bacteriocenosis of bovine ejaculates. Therefore, this study aims to get a closer look on the effects of Staphylococcus-induced bacteriospermia under in vitro conditions on bovine sperm quality. Prior to inducing bacteriospermia, spermatozoa were separated from each ejaculate using Percoll® Plus gradient medium in order to limit the effects only to the selected bacterial species. Seven Staphylococcus species previously isolated from bovine semen were used for our experiments at a turbidity of 0.5 McFarland (equivalent to 1.5 × 108 colony-forming units per mL). The contaminated semen samples were incubated at 37 °C and at times of 0, 2, and 4 h, motility, mitochondrial membrane potential, reactive oxygen species (ROS) generation, sperm DNA fragmentation, and magnesium (Mg) and calcium (Ca) extracellular concentration were analyzed and compared with the control group (uncontaminated). The results showed no significant changes at the initial measurement. However, significant adverse effects were observed after 2 h and 4 h of incubation. Most notably, the presence of S. aureus, S. warneri, S. kloosii, and S. cohnii caused a significantly increased ROS production, leading to sperm DNA fragmentation, changes in the mitochondrial membrane potential, and a decreased sperm motility. Furthermore, the presence of Staphylococcus species led to lower extracellular concentrations of Mg and Ca. In conclusion, the overgrowth of Staphylococcus bacteria in bovine semen may contribute to oxidative stress resulting in sperm DNA fragmentation, altered mitochondrial membrane potential, and diminished sperm motility.

show abstract

Section: Discussionmentioning

confidence: 99%

Staphylococcus-Induced Bacteriospermia In Vitro: Consequences on the Bovine Spermatozoa Quality, Extracellular Calcium and Magnesium Content

Ďuračka

Husarčíková

Jančov

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…Other authors found 103-106 CFU/mL in five bulls analysed after collection at an insemination centre [59] and 50.38 ± 16.29 CFU/mL in thawed cryopreserved bull semen collected under field conditions [60]. A recent study [61] isolated 135 microorganisms from 25 genera from 174 frozen-thawed semen collected from artificial vaginas and analysed at different periods of the conservation process. However, there is scarce information about the microbiological quality of semen collected in the field.…”

Section: Discussionmentioning

confidence: 99%

“…In one study [61], all 55 microorganisms evaluated were resistant to penicillin, contained in INRA96 ® , and all but one microorganism were resistant to tylosin and lincomycin, both present in BIOXcell ® and AndroMed ® . In a study of bull semen, resistance to all tested antibiotics was observed in 22% of all isolates from 135 different microorganisms, whereas only 3.9% of the isolates were inhibited by the antibiotics stipulated in EC Directive 88/407 [61]. A study on the bacterial load in frozen bull semen found that Gram-negative and -positive bacteria behaved differently in response to different antibiotics [62].…”

Section: Discussionmentioning

confidence: 99%

Effects of Extender Type, Storage Time, and Temperature on Bull Semen Parameters

Fernández-Novo

Santos-Lopez

Barrajon-Masa

et al. 2021

Biology

View full text Add to dashboard Cite

Seminal parameters can be evaluated in situ, or samples can be delivered to a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on semen quality. Acrosome integrity, sperm viability and morphology, CASA-total and progressive motility, pH, and colony-forming units were assessed. Semen quality was preserved for up to 4–6 h post-ejaculation, except for INRA96® at 5 °C. Regardless of extender or temperature, motility decreased from 4 to 6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. pH differed from 4 to 6 h up to 24 h, acidifying when stored at room temperature. Microbiological load was stable over time with AndroMed® and BIOXcell®, and increased at room temperature with INRA96®. Our results suggest that AndroMed® and BIOXcell® can preserve semen quality for up to 6 h, either at 5 °C or room temperature, while INRA96® only at room temperature. These results help to fix adequate protocols for short-term storage and shipment of bovine semen collected under field conditions.

show abstract

“…and Pseudomonas spp. [ 82 ]. Similar resistance patterns were also observed in semen samples collected from boars [ 83 ] and rams [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Attenuates Damage to Rooster Spermatozoa Exposed to Selected Uropathogens

Tvrdá

Petrovičová²,

Benko³

et al. 2022

Pharmaceutics

View full text Add to dashboard Cite

Artificial insemination, as an essential pillar of the modern poultry industry, primarily depends on the quality of semen collected from stud roosters. Since the collection and storage of ejaculates is not a sterile process, antimicrobial agents have become essential supplements to semen extenders. While the use of traditional antibiotics has been challenged because of rising bacterial resistance, natural biomolecules represent an appealing alternative because of their antibacterial and antioxidant properties. As such, this study strived to compare the effects of 50 μmol/L curcumin (CUR) with 31.2 µg/mL kanamycin (KAN) as a conventional antibiotic on rooster sperm quality in the presence of Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa. Changes in sperm structural integrity and functional activity were monitored at 2 and 24 h of culture. Computer-assisted semen analysis revealed significant sperm motility preservation following treatment with KAN, particularly in the case of Salmonella enterica and Pseudomonas aeruginosa (p < 0.001) after 24 h. On the other hand, CUR was more effective in opposing ROS overproduction by all bacteria (p < 0.05), as determined by luminol-based luminometry, and maintained sperm mitochondrial activity (p < 0.001 in the case of Salmonella enterica; p < 0.05 with respect to Escherichia coli and Pseudomonas aeruginosa), as assessed by the fluorometric JC-1 assay. The TUNEL assay revealed that CUR readily preserved the DNA integrity of rooster sperm exposed to Salmonella enterica (p < 0.01) and Escherichia coli (p < 0.001). The bacteriological analysis showed higher efficiency of KAN in preventing the growth of all selected bacterial species (p < 0.0001) as opposed to CUR. In conclusion, CUR provided protection to rooster spermatozoa against alterations caused by uropathogens, most likely through its antioxidant activity. Hence, CUR supplementation to poultry semen extenders in combination with properly selected antibacterial substances may become an interesting strategy in the management of bacterial contamination during semen storage.

show abstract

Antibiotic resistance in microorganisms isolated in a bull semen stud

Cited by 12 publications

References 20 publications

Staphylococcus-Induced Bacteriospermia In Vitro: Consequences on the Bovine Spermatozoa Quality, Extracellular Calcium and Magnesium Content

Staphylococcus-Induced Bacteriospermia In Vitro: Consequences on the Bovine Spermatozoa Quality, Extracellular Calcium and Magnesium Content

Effects of Extender Type, Storage Time, and Temperature on Bull Semen Parameters

Curcumin Attenuates Damage to Rooster Spermatozoa Exposed to Selected Uropathogens

Contact Info

Product

Resources

About