Antibiotic Resistance in Human Chronic Periodontitis Microbiota

Rams, Thomas E.; Degener, John E.; Winkelhoff, A.J. van

doi:10.1902/jop.2013.130142

Cited by 214 publications

(193 citation statements)

References 44 publications

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“…Never the less, based on our preliminary findings,one may postulate that the use of a triple antibiotic medicament might help in eliminating bacteria in the periodontally affected compartment, especially because a wide variability in periodontal pathogen antibiotic resistance pattern can be found, which is of critical concern to clinicians when empirically selecting antibiotic treatment regimens for periodontitis patients [46][47][48]. In root filled and endodontic infected teeth, biofilm formation can be observed and subsequently results in a reservoir for antibiotic resistance [49].…”

Section: Discussionmentioning

confidence: 99%

“…It must be considered that antibiotic diffusion through dentine could lead to subinhibitory concentrations on the infected sites that might, in turn, induce bacterial resistance especially when the biofilm is intact. An adequate concentration [55] after diffusion through dentine must be guaranteed. Therefore further diffusion studies including biofilm formation are needed.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

et al. 2015

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OBJECTIVES This study aims to assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model. MATERIALS AND METHODS Fifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized, and randomly allocated to four groups (n = 10/group) according to their intracanal medication: sterile saline (NaCl; control, A); the TVM carrier material alone, i.e., propylene glycol (PG; B); TVM (C); and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidious Anaerobe Agar, inoculated with Fusobacterium nucleatum suspension and then anaerobically incubated during 48-h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm(2)). RESULTS Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on disinfection (p = 0.9320). CONCLUSION TVM was able to penetrate through the dentine and inhibit the bacterial growth of F. nucleatum up to 192 h. CLINICAL RELEVANCE TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent. Declaration of interests:The authors declare they have no conflicts of interest. AbstractObjectives To assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface applying a new in vitro model. Materials and MethodsFifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized and randomly allocated to four groups (n=10/group) according their intracanal medication: Sterile saline NaCl (control, A), the TVM carrier material alone, i.e. propylene glycol (PG; B), TVM (C) and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidiuos Anaerobe Agar, inoculated with F. nucleatum suspension and then anaerobically incubated during 48 h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm 2 ).Results Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on the disinfection (p = 0.9320).Conclusion TVM was able to penetrate through dentine and inhibit the bacterial growth of F. nucleatum up to 192 h.Clinical relevance TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

et al. 2015

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“…This treatment modality eliminates the entire microbiota, irrespective of its pathogenicity. Also, it could generate antibiotic resistance and recolonization of treated sites with pathogenic bacteria is frequent [112,113]. Hence, there is a need of new treatment paradigms in periodontal disease management.…”

Section: Probiotics and Periodontitismentioning

confidence: 99%

Probiotics and Periodontal Diseases

Morales¹,

Bravo²,

Bedoya³

et al. 2017

Insights Into Various Aspects of Oral Health

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Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Probiotics have been used to directly modify the resident oral microbiome and proposed to modulate immune responses. In dentistry, probiotics have been employed as useful adjuncts for the reduction of caries development, suppressing oral Candida infection and controlling halitosis. Plaque-induced gingivitis is a gingival inflammation caused by the adherent bacterial biofilm around teeth. Gingivitis and periodontitis are considered to be a continuum of the same inflammatory process, although many gingivitis lesions do not progress to periodontitis. Periodontitis is an inflammatory process that affects the attachment structures of teeth. It constitutes a second cause of tooth loss worldwide. Conventional treatment modalities of periodontal disease include non-surgical and/or surgical management, with an emphasis on mechanical debridement. However, mechanical debridement as a sole therapy is not always effective to improve clinical parameters. A growing number of studies support probiotic therapy to prevent or treat gingivitis and periodontitis. Oral administration of probiotics is an effective adjunct in reducing pathogenic bacteria and improving clinical signs of disease. Probiotics may serve as adjunct or replacement therapy substitute antibiotics in managing human periodontal infections in future.

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“…Dental biofilms may serve as a reservoir of bacteria and antimicrobial resistance determinants [3]. Antibiotic-resistant bacteria, such as those that produce β-lactamase, are more prominent in periodontitis [4]. The toxic side effects of antibiotics that may be effective in in vitro susceptibility assays can cause organ damage and selection of resistant bacterial strains.…”

Section: Descriptionmentioning

confidence: 99%