Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture

Luprano, Maria Laura; Sanctis, Marco De; Moro, Guido Del; Iaconi, Claudio Di; López, Antonio; Levantesi, Caterina

doi:10.1016/j.scitotenv.2016.07.055

Cited by 46 publications

(23 citation statements)

References 51 publications

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“…Recently PAA disinfection was studied in combination with the sequential batch biofilter granular reactor (SBBGR) and it was found that with a dose of 1 mg/L of PAA it was possible to reach E. coli < 10 CFU / 100 mL [143]. Furthermore, SBBGR was able to reduce the amount of antibiotic resistance genes (ARGs), whereas the PAA had no impact on ARGs [144].…”

Section: Primary Secondary and Tertiary Wastewater Effluentsmentioning

confidence: 99%

Peracids in water treatment: A critical review

Luukkonen

Pehkonen

2016

Critical Reviews in Environmental Science and Technology

255

122

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Peracids have gained interest in the water treatment over the last few decades. Peracetic acid (CH 3 CO 3 H) has already become an accepted alternative disinfectant in wastewater disinfection whereas performic acid (CHO 3 H) has been studied much less, although it is also already commercially available. Peracids have also been tested for drinking water disinfection, oxidation of aqueous (micro)pollutants, sludge treatment and ballast water treatment, to name just a few examples. The purpose of this review paper is to represent comprehensive up-to-date information about the water treatment applications, aqueous reaction mechanisms, and disinfection byproduct formation of peracids, namely performic, peracetic and perpropionic acids. and PFA have several of the qualities of an ideal disinfectant: toxicity to microorganisms, but not to higher forms of life; effectivity at ambient temperatures; stability and long shelf-life; low corrosivity; deodorizing ability; widespread availability and reasonable cost [12].PAA and PFA are colorless liquids with characteristic pungent odors. Both are thermodynamically unstable and can decompose spontaneously or explode when highly concentrated, heated, under mechanical stress or exposed to catalytic effects of impurities [1,13,14]. The recommended storage temperatures are below 30 and 20°C for PAA and PFA, respectively [6,14]. Longer carbon chain length increases stability, and thus PAA is more stable than PFA [1]. However, the O-O bond dissociation energy of PFA and PAA has been calculated to be similar: 48 kcal/mol [15]. Percarboxylic acids have typically pK a values 3-4 units higher than the parent carboxylic acids [1]. Nonetheless, PFA and PAA are corrosive [16]. ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 3Exposure to PAA and PFA causes irritation and possibly permanent damage to the skin (cutaneous emphysema), eyes and the respiratory system. In the skin contact, 0.2% is proposed as the no-observed-effect concentration (NOEC) for short and medium length exposure [17].When inhaled, airborne concentrations less than 0.16-0.17 ppm (0.50-0.52 mg/m 3 ) are considered to cause no irritation [17,18]. However, higher concentrations are harmful and a case study suggested that PAA could cause occupational asthma [19].The largest users of PAA on a global scale (estimated in 2013) are shown in Fig. 1. Similar numbers are not available for PFA, but it is probably used at significantly lower quantities.The largest user, the food industry, applies PAA as a disinfectant in fresh produce washing water, clean-in-place (CIP) processes, on food processing equipment, and in pasteurizers, to name just a few examples [21,22]. PFA is also a suitable disinfectant in low-temperature refrigerated food processing and storage rooms [23]. In healthcare, PAA is used for instance in Recently, methods to determine the residual PAA from wastewater were compared: the spectrophotometric method using DPD and catalase was recommended for 0.1-0.5 mg/L PAA concentrations and the cerimetric/iodometric titration...

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Section: Primary Secondary and Tertiary Wastewater Effluentsmentioning

confidence: 99%

Peracids in water treatment: A critical review

Luukkonen

Pehkonen

2016

Critical Reviews in Environmental Science and Technology

255

122

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“…This result is consistent with reports that PAA did not enhance overall ARG removal (i.e., reduce total ARG concentrations measured by qPCR) in post-secondary treated WW effluent after disinfection. 15,16 The application of vPCR in this study incorporates the specificity of qPCR and overcomes the limitation of qPCR to differentiate between DNA originating from viable and nonviable sources. PAA’s mode of action is presumed to be oxidization of sulfhydryl and sulfur bonds in proteins, enzymes and other metabolites, including those in microbial membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Two recent studies demonstrated that PAA is not effective at reducing the total concentrations of tet (A), bla TEM , qnr S, erm B, sul 1, and sul 2 genes encoding resistance to tetracycline, beta-lactams, quinolone, erythromycin and sulfonamides respectively, in secondary WW effluents. 15, 16 Flow cytometry analysis with live-dead staining demonstrated damage to cell walls indicating that a portion of detected ARGs were extracellular or present in nonviable cells. 15 Little data is available reporting disinfection kinetics for ARG carrying cells in WW, 17 and none is available for CSO effluent.…”

Section: Introductionmentioning

confidence: 99%

Peracetic acid disinfection kinetics for combined sewer overflows: indicator organisms, antibiotic resistance genes, and microbial community

Eramo

Medina

Fahrenfeld

2017

Environ. Sci.: Water Res. Technol.

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Combined sewer overflows (CSOs) degrade water quality and end-of-pipe treatment is one potential solution for retrofitting this outdated infrastructure. The goal of this research was to evaluate peracetic acid (PAA) as a disinfectant for CSOs using viability based molecular methods for antibiotic resistance genes (ARGs), indicator organism marker gene BacHum, and 16S rRNA genes. Simulated CSO effluent was prepared using 23–40% wastewater, representing the higher end of the range of wastewater concentrations reported in CSO effluent. PAA residual following disinfection was greatest for samples with the lowest initial COD. Treatment of simulated CSO effluent (23% wastewater) with 100 mg∙min/L PAA (5 mg/L PAA, 20 min) was needed to reduce viable cell sul1, tet(G), and BacHum (1.0±0.63–3.2±0.25-log) while 25 to 50 mg•min/L PAA (5 mg/L PAA, 5–10 min) was needed to reduce viable cell loads (0.62±0.56–1.6±0.08-log) in 40% wastewater from a different municipal treatment plant. Increasing contact time after the initial decrease in viable cell gene copies did not significantly improve treatment. A much greater applied Ct of 1200 mg∙min/L PAA (20 mg/L PAA, 60 min) was required for significant log reduction of 16S rRNA genes (3.29±0.13-log). No significant losses of mexB were observed during the study. Data were fitted to a Chick-Watson model and resulting inactivation constants for sul1 and tet(G) > BacHum > 16S rRNA. Amplicon sequencing of the 16S rRNA gene indicated the initial viable and total microbial communities were distinct and that treatment with PAA resulted in marked increases of the relative abundance of select phyla, particularly Clostridia which increased by 1–1.5 orders of magnitude. Results confirm that membrane disruption is a mechanism for PAA disinfection and further treatment is needed to reduce total ARGs in CSO effluent.

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“…Kümmerer [7] considers antibiotics substances require special attention because they have different molecular structures along with diverse physical, chemical and biological characteristics and also because they behave in different ways with pH variation, such as: neutral, cationic, anionic or zwitterionic. Antibiotic resistance genes were found to many antibiotics, including Vancomym by Luprano [8] and Çardak [9]. Antibiotics are subdivided into several groups: Quinolones, β-Lactams, Tetracyclines, Macrolides and Glycopeptides.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Vancomycin Hydrochloride by Electrooxidation

Mh¹,

Ars²,

Wa³

2017

Mod Chem Appl

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Many pharmaceuticals are considered recalcitrant pollutants and represent a problem for human and animal health due to continuous contribution in the aquatic environment and the bacteria resistance development to antibiotics. Vancomycin is one of the most antibiotics administered in medicine, nevertheless, there is not much knowledge about the presence, fate and the effects of Vancomycin in the environment. The advanced oxidation techniques (AOTs) are shown as alternatives for the treatment of water and wastewater in order to degrade pollutants and contaminants. Therefore, this work is aimed to evaluate the Vancomycin degradation in an aqueous medium using electrooxidation (EO) technique and to optimize the reaction conditions. The experiments were conducted in a homemade electrochemical cell in acrylic with a working volume of 500 cm 3 ; DSA electrodes -Dimensionally Stable Anodes (70TiO 2 -30RuO 2 ) were used (effective area of work -41.25 cm 2 ); NaCl P.A. was used as supporting electrolyte; 130 mg L -1 of Vancomycin was used in aqueous solution. The optimization was done using central composite design (CCD) totaling 18 experiments. The evaluated factors were: interelectrodes distance (ID), applied current and supporting electrolyte concentration. All experiments were conducted for 20 minutes with sampling at 0, 1, 2, 3, 4, 5, 10 and 20 minutes.

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Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture

Cited by 46 publications

References 51 publications

Peracids in water treatment: A critical review

Peracids in water treatment: A critical review

Peracetic acid disinfection kinetics for combined sewer overflows: indicator organisms, antibiotic resistance genes, and microbial community

Degradation of Vancomycin Hydrochloride by Electrooxidation

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