Antibiotic Resistance Gene Transfer between Streptococcus gordonii and Enterococcus faecalis in Root Canals of Teeth Ex Vivo

Sedgley, Christine M.; Lee, Esther H.; Martin, Matthew J.; Flannagan, Susan E.

doi:10.1016/j.joen.2008.02.014

Cited by 69 publications

(46 citation statements)

References 35 publications

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“…E. faecalis was also detected with a high prevalence in primary endodontic infections (29). Antibiotic resistance in E. faecalis and E. faecium and a possible contribution to horizontal gene transfer as a result of this resistance underline the growing attention being paid to enterococci in the oral cavity (30,31). Moreover, E. faecalis is one of the most discussed microorganisms in endodontic infections and has been shown to possess different putative virulence factors (27,32), which were also found in endodontic strains (15).…”

Section: Discussionmentioning

confidence: 99%

Food-borne Enterococci Integrate Into Oral Biofilm: An In Vivo Study

Al‐Ahmad

Maier

Follo

et al. 2010

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Food-borne Enterococci Integrate Into Oral Biofilm: An In Vivo Study

Al‐Ahmad

Maier

Follo

et al. 2010

Journal of Endodontics

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“…Consequently, their response to antimicrobial treatment may differ from the response seen with a single species of bacteria (33)(34)(35). Further investigation of microbial biofilm resistance to antibiotics in cases of endodontic infections is needed.…”

Section: Clinical Researchmentioning

confidence: 99%

Susceptibility of Endodontic Pathogens to Antibiotics in Patients with Symptomatic Apical Periodontitis

Skučaitė

Pečiulienė

Vitkauskienė

et al. 2010

Journal of Endodontics

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“…Previous studies indicated that although it is unable to replicate in nonenterococcal species, pAM373 may deliver a plasmid-borne transposon (10). It may also facilitate or enhance the mobilization of other, broader-host-range plasmids that have been shown to become established following transfer between E. faecalis and S. gordonii (12,51) or persist in recipients as part of a cointegrate structure bearing a replicative component (26,54).…”

mentioning

confidence: 99%

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

et al. 2010

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Enterococcus faecalis strains secrete multiple peptides representing different sex pheromones that induce mating responses by bacteria carrying specific conjugative plasmids. The pheromone cAM373, which induces a response by the enterococcal plasmid pAM373, has been of interest because a similar activity is also secreted by Streptococcus gordonii and Staphylococcus aureus. The potential to facilitate intergeneric DNA transfer from E. faecalis is of concern because of extensive multiple antibiotic resistance, including vancomycin resistance, that has emerged among enterococci in recent years. Here, we characterize the related pheromone determinant in S. gordonii and show that the peptide it encodes, gordonii-cAM373, does indeed induce transfer of plasmid DNA from E. faecalis into S. gordonii. The streptococcal determinant camG encodes a lipoprotein with a leader sequence, the last 7 residues of which represent the gordonii-cAM373 heptapeptide SVFILAA. Synthetic forms of the peptide had activity similar to that of the enterococcal cAM373 AIFILAS. The lipoprotein moiety bore no resemblance to the lipoprotein encoded by E. faecalis. We also identified determinants in S. gordonii encoding a signal peptidase and an Eep-like zinc metalloprotease (lspA and eep, respectively) similar to those involved in processing certain pheromone precursors in E. faecalis. Mutations generated in camG, lspA, and eep each resulted in the ablation of gordonii-cAM373 activity in culture supernatants. This is the first genetic analysis of a potential sex pheromone system in a commensal oral streptococcal species, which may have implications for intergeneric gene acquisition in oral biofilms.

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Antibiotic Resistance Gene Transfer between Streptococcus gordonii and Enterococcus faecalis in Root Canals of Teeth Ex Vivo

Cited by 69 publications

References 35 publications

Food-borne Enterococci Integrate Into Oral Biofilm: An In Vivo Study

Food-borne Enterococci Integrate Into Oral Biofilm: An In Vivo Study

Susceptibility of Endodontic Pathogens to Antibiotics in Patients with Symptomatic Apical Periodontitis

A Genetic Determinant in Streptococcus gordonii Challis Encodes a Peptide with Activity Similar to That of Enterococcal Sex Pheromone cAM373, Which Facilitates Intergeneric DNA Transfer

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