Antibiotic resistance gene dissipation in soil microcosms amended with antibiotics and swine manure

Alt, Laura M.; Iverson, Alyssa N.; Soupir, Michelle L.; Howe, Adina

doi:10.1002/jeq2.20240

Cited by 6 publications

(4 citation statements)

References 69 publications

(86 reference statements)

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“…adjacent soils and waters (Peng et al, 2017;Zhang et al, 2021), we detected these genes in all 48 manures in this The concentrations measured in our study were consistent with those detected in manure holding pits measured in other studies (Mackie et al, 2006;Joy et al, 2013;Hall et al, 2020;Alt et al, 2021) and also demonstrate the wide variations of ARGs that can be observed within manures, with variations up to three-fold. The wide ranges of measured ermB and tetM in these manures may be caused by covariates in manure holding pits that have yet unknown implications on ARG concentrations after long-term exposure such as concentrations of heavy metals, manure pit additives, or changes in chemical properties such as pH or organic substrates (Hölzel et al, 2012;He et al, 2020).…”

Section: Concentrations Of Ermb and Tetm In Swine Manure Pits (Conven...supporting

confidence: 92%

“…In this study, we expand our knowledge of the presence of antibiotic resistant determinants in swine manure by providing a broad comparison of ARGs among manures from 48 farms. We aimed to quantify the presence of ARGs that have been demonstrated to be consistently enriched in swine manures, tetM and ermB (Whitehead and Cotta, 2013;Wen et al, 2019;Alt et al, 2021) and also characterized the presence of diverse resistance genes associated with other antibiotics and with swine manure, including aminoglycoside, carbapenem, lincosamide, phenicol, and sulfonamide resistance (Table 1). Our justification for the gene selection is that these genes are associated with the most sold antibiotics in swine production (Center for Veterinary Medicine, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of antibiotic resistance genes in swine manure storage pits of Iowa, USA

Neher¹,

Soupir²,

Andersen³

et al. 2023

Front. Antibiot.

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Antimicrobial resistance (AMR) can develop in deep-pit swine manure storage when bacteria are selectively pressured by unmetabolized antibiotics. Subsequent manure application on row crops is then a source of AMR into soil and downstream runoff water. Therefore, understanding the patterns of diverse antibiotic resistance genes (ARGs) in manure among different farms is important for both interpreting the results of the detection of these genes from previous studies and for the use of these genes as bioindicators of manure borne antibiotic resistance in the environment. Previous studies of manure-associated ARGs are based on limited samples of manures. To better understand the distribution of ARGs between manures, we characterized manures from 48 geographically independent swine farms across Iowa. The objectives of this study were to characterize the distribution of ARGs among these manures and to evaluate what factors in manure management may influence the presence of ARGs in manures. Our analysis included quantification of two commonly found ARGs in swine manure, ermB and tetM. Additionally, we characterized a broader suite of 31 ARGs which allowed for simultaneous assays of the presence or absence of multiple genes. We found the company integrator had a significant effect on both ermB (P=0.0007) and tetM gene concentrations (P=0.0425). Our broad analysis on ARG profiles found that the tet(36) gene was broadly present in swine manures, followed by the detection of tetT, tetM, erm(35), ermF, ermB, str, aadD, and intl3 in samples from 14 farms. Finally, we provide a comparison of methods to detect ARGs in manures, specifically comparing conventional and high-throughput qPCR and discuss their role in ARG environmental monitoring efforts. Results of this study provide insight into commonalities of ARG presence in manure holding pits and provide supporting evidence that company integrator decisions may impact ARG concentrations.

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Section: Concentrations Of Ermb and Tetm In Swine Manure Pits (Conven...supporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Comparison of antibiotic resistance genes in swine manure storage pits of Iowa, USA

Neher¹,

Soupir²,

Andersen³

et al. 2023

Front. Antibiot.

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“…The tetM gene has the widest bacteria host range of all tetracycline genes and is considered highly transferable, being located on transposons and integrases (Roberts, 2005). A previous study reported tetM gene copies as three times more abundant than 16S genes in a swine manure storage pit in Iowa (Alt et al, 2021). Notably, TET genes were present in all manure samples, with the highest number of different genes detections in samples 12 and 13 (Figure 3).…”

Section: Antimicrobial Resistance Gene Detectionsmentioning

confidence: 91%

Co-occurrence of antimicrobials and metals as potential drivers of antimicrobial resistance in swine farms

Congilosi

Wallace

Neher

et al. 2022

Front. Environ. Sci.

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The application of animal manures to cropland is an important nutrient recycling strategy in many parts of the world. Commonly, aggregated manure wastes contain chemical stressors including veterinary antimicrobials, heavy metals, and antimicrobial resistance genes (ARGs) that can stimulate the development and proliferation of antimicrobial resistance (AMR). While the presence of antimicrobials in manure is well-documented, the co-occurrence of other potentially impactful chemical stressors in swine manure remains underreported. This study quantifies and analyzes correlations between antimicrobials, metals, and certain ARGs present in manure samples from swine farms in Iowa, United States. Relationships between chemical stressors and different stages of swine production or feed composition are also investigated. Results revealed substantial levels of tetracyclines [up to 1,260 µg g−1 dry weight (d.w.) of manure for oxytetracycline] detected in all samples. Tiamulin, two ionophores (monensin and lasalocid), and one macrolide (tilmicosin) were detected at maximum class concentrations of 9.4, 0.547, and 0.472 µg g−1 d.w., respectively. The median relative abundances of ermB and tetM were 0.13 and 0.17 copies g−1 wet weight (w.w.) manure (normalized to 16S gene), respectively. Additionally, high levels of copper (Cu), iron (Fe), and zinc (Zn) were detected in all samples, with maximum concentrations of 887, 1,900, and 2,100 µg g−1 d.w., respectively. Notably, uranium (U) was detected in 11 samples, at concentrations up to 0.77 µg g−1. A global analysis of AMR-stressor relationships using Spearman’s rank correlation indicates Cu, and Ba are the most positively and significantly correlated with cytotoxic anhydrotetracycline (ATC) and/or anhydrochlortetracycline (ACTC) concentrations in all tested facilities (Cu-ATC: ρ = 0.67, p = 0.0093; Cu-ACTC: ρ = 0.75, p = 0.0022; Ba-ATC: ρ = 0.84, p = 0.0002). Interestingly, ermB and tetM genes were strongly, positively correlated to each other (ρ = 0.92, p < 0.0001), suggesting possible co-selection, despite the absence of correlation between ARGs and tetracycline concentrations. This study demonstrates the complexity of interactions between antimicrobials, metals, and ARGs in multiple manure storage pits prior to cropland application.

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“…Antibiotic pollution of farmland soil has become a key factor influencing the sustainability of agricultural ecosystems [ 1 , 2 ]. The application of animal manure and manure-based fertilizers is the main way for antibiotics to enter the farmland soil, due to the high residual concentrations of antibiotics in animal feces or urine in intensive farming systems [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Ecological Responses of Maize Rhizosphere to Antibiotics Entering the Agricultural System in an Area with High Arsenicals Geological Background

Chen

Feng

et al. 2022

IJERPH

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Metal(loid)s can promote the spread and enrichment of antibiotic resistance in the environmental ecosystem through a co-selection effect. Little is known about the ecological effects of entering antibiotics into the environment with long-term metal(loid)s’ resistance profiles. Here, cow manure containing oxytetracycline (OTC) or sulfadiazine (SA) at four concentrations (0 (as control), 1, 10, and 100 mg/kg) was loaded to a maize cropping system in an area with high a arsenicals geological background. Results showed that exogenous antibiotics entering significantly changed the nutrient conditions, such as the concentration of nitrate nitrogen, ammonium nitrogen, and available phosphorus in the maize rhizosphere soil, while total arsenic and metals did not display any differences in antibiotic treatments compared with control. Antibiotics exposure significantly influenced nitrate and nitrite reductase activities to reflect the inhibition of denitrification rates but did not affect the soil urease and acid phosphatase activities. OTC treatment also did not change soil dehydrogenase activities, while SA treatment posed promotion effects, showing a tendency to increase with exposure concentration. Both the tested antibiotics (OTC and SA) decreased the concentration of arsenite and arsenate in rhizosphere soil, but the inhibition effects of the former were higher than that of the latter. Moreover, antibiotic treatment impacted arsenite and arsenate levels in maize root tissue, with positive effects on arsenite and negative effects on arsenate. As a result, both OTC and SA treatments significantly increased bioconcentration factors and showed a tendency to first increase and then decrease with increasing concentration. In addition, the treatments decreased translocation capacity of arsenic from roots to shoots and showed a tendency to increase translocation factors with increasing concentration. Microbial communities with arsenic-resistance profiles may also be resistant to antibiotics entering.

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Antibiotic resistance gene dissipation in soil microcosms amended with antibiotics and swine manure

Abstract: Antibiotic resistant gene dissipation in soil microcosmsCore ideas (3-5 impact statements, 85 char max for each)The dissipation of manure associated ARGs was not impacted by soil vegetative growth.Five of six quantified ARGs dissipated over time with half-lives ranging from 4.7 to 10.7 d.

Cited by 6 publications

References 69 publications

Comparison of antibiotic resistance genes in swine manure storage pits of Iowa, USA

Comparison of antibiotic resistance genes in swine manure storage pits of Iowa, USA

Co-occurrence of antimicrobials and metals as potential drivers of antimicrobial resistance in swine farms

Ecological Responses of Maize Rhizosphere to Antibiotics Entering the Agricultural System in an Area with High Arsenicals Geological Background

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