Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital

Abstract

Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from in-patients in different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority of the antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics, macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. To shed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genome of this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are both chromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibiotic and biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens. The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts.

<i>Pseudomonas oleovorans</i> Sepsis in a Child: The First Reported Case in India

“…Gene transfer has been well studied in P. putida, and a similar mechanism may be present in P. oleovorans. Therefore, the proper identification of these organisms is essential (10,11).…”

Identification and determination of characteristics of endophytes from rice plants and their role in biocontrol of bacterial blight caused by Xanthomonas oryzae pv. oryzae

“…and P. putida as antagonists of Xoo on rice, these bacteria are potentially dangerous and harmful to human health (Brenner et al, 1986;Bouallegue et al, 2004;Berg et al, 2005;Flores-Tena et al, 2007;Molina et al, 2011;Thomas et al, 2013;Molina et al, 2014). Hence the hazardous properties of the bacteria and human safety issues should also be taken into account when considering their practical use as biological control agents in plant protection.…”

Phytotoxic analysis of the impact of transmission oil contaminated soil on germination and growth of oat (<i>Avena sativa</i>) seedlings under different soil moisture conditions

“…Five pieces of Oat seeds each were sown into Petri dishes containing 20 g of the derno-podzoluivisolic soil samples according to the experimental scheme (Table 2). Soil Moisture Contents were estimated using the method of Ogbaga and co-workers (Nwachukwu, 2001;Molina, 2014). The plates were incubated for 7 days at room temperature, after which seedling germination (in %) and growth (in cm) were analyzed.…”

“…It has been shown to be useful in bioremediation evidenced in its naphthalenedegrading ability and its adaptability to different habitats and nutritional environments (Gomes, 2005;Molina, 2014). Further, the role of Pseudomonas putida as a bioremediating agent was studied using soil from the central region of Russia.…”