Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Prashanth, K; Vasanth, T.; Saranathan, Rajagopalan; Makki, Abhijith; Pagal, Sudhakar

doi:10.5772/28813

Cited by 13 publications

(10 citation statements)

References 115 publications

(147 reference statements)

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“… 128 , 131 It is the second RND type efflux pump in A. baumannii and comprises adeI, adeJ , and adeK genes. 132 The AdeIJK pump contributes to resistance to beta-lactams, chloramphenicol, tetracycline, erythromycin, lincosamides, fluoroquinolones, fusidic acid, novobiocin, rifampin, trim-ethoprim, acridine, safranin, pyronine, and sodium dodecyl sulfate. 128 It was suggested that over-expression of AdeIJK contributes largely to multidrug resistance, decreased bio-film production, and altered membrane composition in A. baumannii .…”

Section: Efflux Systemsmentioning

confidence: 99%

<em>Acinetobacter baumannii</em> biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments

Eze

Chenia

Zowalaty

2018

IDR

191

192

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Acinetobacter baumannii is a leading cause of nosocomial infections due to its increased antibiotic resistance and virulence. The ability of A. baumannii to form biofilms contributes to its survival in adverse environmental conditions including hospital environments and medical devices. A. baumannii has undoubtedly propelled the interest of biomedical researchers due to its broad range of associated infections especially in hospital intensive care units. The interplay among microbial physicochemistry, alterations in the phenotype and genotypic determinants, and the impact of existing ecological niche and the chemistry of antimicrobial agents has led to enhanced biofilm formation resulting in limited access of drugs to their specific targets. Understanding the triggers to biofilm formation is a step towards limiting and containing biofilm-associated infections and development of biofilm-specific countermeasures. The present review therefore focused on explaining the impact of environmental factors, antimicrobial resistance, gene alteration and regulation, and the prevailing microbial ecology in A. baumannii biofilm formation and gives insights into prospective anti-infective treatments.

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Section: Efflux Systemsmentioning

confidence: 99%

<em>Acinetobacter baumannii</em> biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments

Eze

Chenia

Zowalaty

2018

IDR

191

192

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“…That indicates that antibiotics impact the quorum sensing system to regulate the cell density to develop biofilm or escape from severe environments. 37 Besides these, translation related proteins, such as 22 ribosome subunits, ribosome-recycling factor (A0KHG6), translation initiation factors IF-1 and IF-2(R4VFM8 and R4W0U9), and elongation factors (R4VT42 and R4VDH4), were increased in OXY stress (Table 1 and Table S1, Supporting Information). Meanwhile, we also identified five outer membranes (R4VRX0, R4VAF7, I3QPT8, A0KI84, and R4VBK9) that increased, which might be related with toxic efflux pump system for bacteria survival.…”

Section: Protein Quantitation Comparisons Of a Hydrophila In And With...mentioning

confidence: 99%

An Integrated Quantitative and Targeted Proteomics Reveals Fitness Mechanisms of Aeromonas hydrophila under Oxytetracycline Stress

Lin

Yao

et al. 2015

J. Proteome Res.

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To date, above ten thousand tons of antibiotics are used in aquaculture each year that lead to the deterioration of natural resources. However, knowledge is limited on the molecular biological behavior of common aquatic pathogens against antibiotics stress. In this study, proteomics profiles of Aeromonas hydrophila, which were exposed to different levels of oxytetracycline (OXY) stress, were displayed and compared using iTRAQ labeling and SWATH-MS based LC-MS/MS methods. A total 1383 proteins were identified by SWATH-MS method, and 2779 proteins were identified from iTRAQ labeling samples. There are 152 up-regulated and 52 down-regulated proteins overlapped in 5 μg/mL OXY stress and both 83 up- and down-regulated proteins overlapped in 10 μg/mL OXY stress in both methods, respectively. Results show that many protein synthesis and translation related proteins increased, while energy generation related proteins decreased in OXY stress. The varieties of selected proteins involved in both pathways were further validated by sMRM(HR), q-PCR, and enzyme activity assay. Furthermore, the concentrations of NAD+ and NADH were measured to verify the characteristic of energy generation process in OXY stress and OXY resistance strain. We demonstrate that the down-regulation of energy generation related metabolic pathways and up-regulation of translation may play an important role in antibiotics fitness or resistance of aquatic pathogens.

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“…A biofilm‐associated protein (Bap) was first characterized in Staphylococcus aureus that causes bovine mastitis and subsequently was detected in A. baumannii . Bap family members are high‐molecular weight proteins present on the bacterial surface, contain a core domain of tandem repeats, and play a critical role in cell–cell interactions, and biofilm formation . It was reported that bap genes play an important role in the overall ability of the bacterium to resist environmental stress like the presence of antibiotics .…”

mentioning

confidence: 99%

Effect of iron on expression of efflux pump (adeABC) and quorum sensing (luxI, luxR) genes in clinical isolates of Acinetobacter baumannii

Modaresi

Azizi

Shakibaie

et al. 2015

APMIS

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Resistance-nodulation-division efflux system (RND) adeABC contributes to intrinsic resistance to various drug classes in Acinetobacter baumannii. Similarly, quorum sensing (QS) plays an important role in the biofilm formation and pathogenicity of this bacterium. The aims of this study were to evaluate the influence of iron limitation on the expression of efflux pump (adeABC) genes and QS (luxI, luxR) system by relative quantitative real-time polymerase chain reaction (qRT-PCR). In addition, DNA sequence and phylogenetic relatedness of biofilm-associated protein (Bap) gene was also investigated. Sixty-five multidrug-resistant isolates of A. baumannii were recovered from ICU patients of three hospitals in Kerman, Iran. The isolates were highly resistant to at least 11 antibiotics (MIC ≥64 μg/mL); however, 87% and 89% were susceptible to colistin and tigecycline, respectively (MIC 0.05 μg/mL) (p ≤ 0.05). We detected the presence of RND efflux pump, QS, and bap genes with the frequencies of 92% (adeA), 61.5% (adeB), 84.6% (adeC), 80% (luxI), 61% (luxR), and 66% (bap), respectively. qRT-PCR analysis showed that in some isolates, expression of both adeABC and luxI/R was increased more than fourfold in the presence of low iron (20 μm), suggesting the additional regulatory role of iron on both efflux pump and QS system. Alignment and phylogenetic analysis on the strong biofilm forming isolates confirmed that the fragments amplified were indeed part of bap gene and deduced sequence was similar to A. baumannii K9B410.

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Antibiotic Resistance, Biofilms and Quorum Sensing in Acinetobacter Species

Cited by 13 publications

References 115 publications

<em>Acinetobacter baumannii</em> biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments

<em>Acinetobacter baumannii</em> biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments

An Integrated Quantitative and Targeted Proteomics Reveals Fitness Mechanisms of Aeromonas hydrophila under Oxytetracycline Stress

Effect of iron on expression of efflux pump (adeABC) and quorum sensing (luxI, luxR) genes in clinical isolates of Acinetobacter baumannii

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