Antibiotic Resistance and Virulence Properties in <i>Escherichia col</i>i Strains from Cases of Urinary Tract Infections

Abstract

This study determined E. coli resistance to commonly used antibiotics together with their virulence properties in Ile-Ife, Nigeria. A total of 137 E. coli isolates from cases of urinary tract infection were tested for their sensitivity to commonly used antibiotics and possession of virulence factors using standard methods. Their ability to transfer resistance was also determined. The isolates demonstrated a high and widespread resistance (51.1 % to 94.3 %) to all the antibiotics used except Nitrofurantoin (7.3 %). A total of 50 (36.5 %) of the isolates were resistant to 10 of the eleven antibiotics employed. Sixty three per cent (63 %) of the 107 trimethoprim resistant E. coli transferred their resistances while amoxicillin, gentamycin, augmentin, tetracycline and erythromycin were cotransferred with trimethoprim. Fifty one (37.2 %) of these multi-resistant isolates possessed one or more virulent factors. The study concluded that urinary tract infection due to E. coli in Ile-Ife may be difficult to treat empirically except with nitrofurantoin, due to high resistance to commonly used antibiotics. It is imperative that culture and susceptibility tests be carried out on infecting pathogen prior to treatment, in order to avoid treatment failure and reduce selective pressure that could result in the spread of uropathogenic E. coli in the environment.

Investigation of P Fimbriae Presence in Escherichia coli Strains Isolated from Urine Samples in Human, and Their Antibacterial Resistance

“…In the study, E. coli isolates were found to be most resistant to ciprofloxacin, trimethoprim sulfamethoxazole, cefazolin, amoxicillinclavulanic acid and norfloxacin, which are the most frequently, used antibiotics in empirical therapy. These findings have also been reported in other studies (18,19). It is recommended to use other antibiotics such as imipenem, aztreonam, colistin in the treatment.…”

“…Extended spectrum beta lactamases can be transferred to other strains via plasmid and transposon, further increasing the significance of this ESBL-resistant isolates (3,15,19 increase in ESBL prevalence from 12.5% to 44.7% in both male and female hospitalized patients, between 2004 -2012 (20). Toka Ozer et al reported the rate of ESBL production in E. coli strains was 13.1% in 2018 (1).…”

Correlation between Biofilm Production and Bacterial Urinary Tract Infections: New Therapeutic Approach

“…However, 44.23 of isolated E. coli were non biofilm producer and 55.77 were able to produce biofilm. In the same context, Olorunmola et al (2013) found that E. coli isolated during UTI demonstrated a high and widespread resistance (51.1 % to 94.3 %) to all the antibiotics used except Nitrofurantoin. The four different isolates of K. pneumonia according to RP (50-92 %) were able to produce biofilm with 72.72% and the percentage of biofilm production of two isolates of P. aeruginosa was 60%.…”

A scaled‐down model for the translation of bacteriophage culture to manufacturing scale

“…Studies have shown that approximately 54% of predominant Escherichia coli strains are resistant to at least one antimicrobial drug (Tadesse et al, ). More recently, a study was carried out where 137 E. coli clinical isolates were tested for resistance to 11 commonly used antibiotics and showed 50 of the isolates tested were resistant to 10 of the 11 antibiotics, highlighting the urgent requirement for alternative therapies (Olorunmola, Kolawole, & Lamikanra, ). More recently, a study sampled 862 clinical isolates of E. coli from a variety of animals including chickens, pigs, and cows and found that 94% of strains were resistant to one drug and 83% were resistant to three antimicrobial classes (Yassin et al, ).…”