Antibiotic resistance and molecular epidemiology of the beta‐lactamase‐producing <i><scp>H</scp>aemophilus influenzae</i> isolated in Chongqing, China

Luo, Chong; Xia, Yongwu; Liu, Qian; Chu, Lijuan; Fu, Xiaohong; Jing, Chunmei; Chen, Dapeng; Liu, Lan; Shi, Yu

doi:10.1111/j.1600-0463.2012.02921.x

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…A previous report in Chongqing, China, noted that bla TEM−1 encoding β-lactamase was the main mechanism of ampicillin-resistance of H. influenzae isolates. Moreover, diversity in the promoter, and the Prpt.b promoter may be related to high resistance of H. influenzae to ampicillin (14). The resistance rate of ampicillin in our study (2015-2018) was 45.10% which was higher than β-lactamase production rates of 33.3%.…”

Section: Discussionmentioning

confidence: 50%

“…Production of β-lactamase was detected by BD BBL TM Cefinase TM (BD laboratories, Franklin Lakes, NJ, USA). The bla TEM−1 and bla ROB−1 genes were identified by PCR as previously reported, respectively (14). The gene ftsI was sequenced and compared with that of Rd KW20 strain to determined the mutation of PBP3 (15).…”

Section: β-Lactamase and Pbp3 Mutation Detectionmentioning

confidence: 99%

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Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Xiao

et al. 2020

Front. Public Health

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Background: The serotype and antimicrobial resistance of Haemophilus influenzae in adult patients have changed due to the application of antimicrobials and H. influenzae type b (Hib) vaccine worldwide. However, the epidemiologic characteristics of H. influenzae in Shanghai are still unavailable. Objective: To determine the serotype distribution, antimicrobial resistance and multilocus sequence type (MLST) of H. influenzae in adult patients in Shanghai. Methods: A total of 51 clinical isolates from adult patients were consecutively collected. Serotypes were determined according to specific capsule gene, bexA, amplified by PCR. Antimicrobial susceptibility test was carried out by the broth microdilution method. β-lactamase production was detected by cefinase disk and the ftsI gene were amplified and sequenced to determine the penicillin binding protein 3 (PBP3) mutation. Molecular epidemiology was performed by MLST analyses. Results: All isolates studied were nontypeable H. influenzae (NTHi) and three of them (5.88%) caused invasive infection. The resistant rates of ampicillin and trimethoprim/sulfamethoxazole were both 45.10%. One third of these isolates produced TEM-1 type β-lactamase and 11.76% were β-lactamase negative ampicillin resistant strains (BLNAR). The PBP3 mutation was detected in 74.51% of the isolates, of which 12 belonged to group III. A total of 36 sequence types (STs) were identified among all isolates. Four isolates of ST103 (7.84%) all produced β-lactamase without mutation of PBP3. Conclusion: H. influenzae infections among adults in Shanghai are predominately caused by NTHi with genetic diversity among adult patients. The prevalence of both β-lactamase production and PBP3 mutation may contribute to high ampicillin resistance rate in Shanghai.

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Section: Discussionmentioning

confidence: 50%

Section: β-Lactamase and Pbp3 Mutation Detectionmentioning

confidence: 99%

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Xiao

et al. 2020

Front. Public Health

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“…All isolates were tested for the production of β-lactamase by the chromogenic cephalosporin nitrocefin method using known β-lactamase positives as controls. The TEM-1 and ROB-1 genes were identified by PCR as previously reported ( 16 ). The deduced amino acid sequence of the PBP3 transpeptidase region was aligned with the corresponding sequence from H. influenzae Rd KW20.…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology and antimicrobial resistance of Haemophilus influenzae in Guiyang, Guizhou, China

Zhou

Wang

Cheng

et al. 2022

Front. Public Health

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BackgroundThe widespread use of antimicrobials and Haemophilus influenzae type b (Hib) vaccine worldwide has altered the epidemiological patterns of invasive H. influenzae. Nonetheless, little is currently known on the epidemiological characteristics of H. influenzae in Guiyang, Guizhou, China.ObjectiveTo determine the serotype distribution, antimicrobial resistance and Multilocus Sequence Typing (MLST) of H. influenzae in hospitalized patients in Guiyang City.MethodsA total of 196 clinical isolates from hospitalized patients were collected. Serotypes were determined according to the specific capsule gene, bexA, amplified by PCR. According to the guidelines of Clinical and Laboratory Standards Institute (CLSI) 2020 drug susceptibility tested, and the results determined. The chromogenic cephalosporin nitrocefin method was used to detect β-lactamase production, β-lactamase negative, ampicillin-resistant (BLNAR) strains were detected by PCR amplification and sequencing of the penicillin-binding protein 3 (PBP3) locus of ftsI. Multilocus Sequence Typing was performed for molecular typing.ResultsAll isolates studied were non-typeable H. influenzae (NTHi). Most patients originated from the pediatrics department (78.6%, 154/196), and suffered from lung with respiratory tract infection (pneumonia and bronchitis, 68.4%, 134/196). The resistance rates of ampicillin, cefaclor and azithromycin were 71.4% (140/196), 36.7% (72/196) and 34.2% (67/196), respectively. 40.3% (79/196) of strains were β-lactamase positive ampicillin-resistant (BLPAR). All BLPAR carried the TEM-1 gene. 9.2% (18/196) were β-lactamase negative ampicillin-resistant strains (BLNAR). The PBP3 mutation was detected in the ampicillin-resistant strains (n = 113), of which 18 belonged to group IIa. A total of 49 sequence types (ST) and 23 clonal complexes (CC) were detected, among which CC107 (ST107, n = 27; ST1002, n = 5; ST1218, n = 5) was the most frequent clonal complexes. BLPAR isolates mostly belonged to ST107 (20/79), while BLNAR was predominantly distributed in ST12 (5/18).ConclusionH. influenzae infections are predominately caused by genetically diverse NTHi among hospitalized patients in Guiyang. The prevalence of β-lactamase production and PBP3 mutation may contribute to the high local ampicillin resistance rate.

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“…In China, the rate of beta-lactamase positive was 35.8% [20]. In another Chinese report, the rate of beta-lactamase positive was 61% and the rate of tetracycline resistant was 94.5% [21].…”

Section: Discussionmentioning

confidence: 98%

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

Minami¹,

Sakakibara²,

Imura³

et al. 2016

JBM

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Haemophilus influenzae is an important pathogen that caused several infection diseases, such as sinusitis, otitis media, sepsis, and meningitis. This study was conducted to find out the prevalence and antimicrobial susceptibility pattern of Haemophilus influenzae isolates at general hospital in the central region of Japan from December 2015 to January 2016. Haemophilus influenzae was identified by standard laboratory procedure. Antimicrobial susceptibility testing was performed by micro dilution assay according to CLSI recommendation. One hundred ninety-one Haemophilus influenzae were isolated, among which 95 (49.7%) were from male and 96 (50.3%) were from female. The age incidence of (0) years, (≤2) years, (≤5) years, and (6≤) years groups were 22(11.5%), 92(48.2%), 61(31.9%), and 16(8.4%), respectively. Positive samples were received mostly from the nasal discharge (177/92.7%), sputum (6/3.1%), tonsillar (6/3.1%), and pharynx (2/1.0%). Ceftriaxone was the most active antibiotics with 100% susceptible rates, followed by ciprofloxacin (99.5%) and minocycline (99%) in our study. Furthermore, we categorized four patterns: beta lactamase-negative ampicillin-sensitive strain (BLNAS), beta lactamase-negative ampicillin-resistant strain (BLNAR), beta lactamase-positive ampicillin resistant strain (BLPAR), and beta lactamase-positive amoxicillin-clavulanic acid-resistant strain (BLPACR) from those ampicillin susceptible results. The numbers of female were significant greater than those of male in BLPAR (p = 0.0336). With respect to antimicrobial susceptible pattern, there was no minocycline and piperacillin resistant strain in both BLNAS and BLNAR (p < 0.0001). Haemophilus influenzae infection spreads worldwide and inadequate use of antibiotics contributes to uptake of their new antimicrobial resistance. Continuous antimicrobial surveys are need for control the emergence and spread of antimicrobial resistance to reduce the morbidity and mortality.

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Antibiotic resistance and molecular epidemiology of the beta‐lactamase‐producing Haemophilus influenzae isolated in Chongqing, China

Cited by 4 publications

References 26 publications

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Molecular Epidemiology and Antimicrobial Resistance of Haemophilus influenzae in Adult Patients in Shanghai, China

Molecular epidemiology and antimicrobial resistance of Haemophilus influenzae in Guiyang, Guizhou, China

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

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