Antibiotic resistance and genetic diversity in water-borne Enterobacteriaceae isolates from recreational and drinking water sources

Kumar, Shailendra; Tripathi, Vinayak Ram; Garg, Shweta

doi:10.1007/s13762-012-0126-7

Cited by 38 publications

(13 citation statements)

References 38 publications

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“…A scoping report on “Antimicrobial Resistance in India” published by Department of Biotechnology, New Delhi, India in 2017 highlighted increasing load of antibiotic-resistant bacteria and ARGs in Indian surface waterbodies and major drinking water sources [ 54 ].Various other studies have also been undertaken across India to check for the presence of antibiotic-resistant bacteria in surface and groundwater sources [ 55 , 56 ]. These studies highlighted that the majority of rivers flowing in Bihar, Goa, Karnataka, Tamilnadu, Telangana states are contaminated with multidrug-resistant E. coli .…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India

Dhawde

Macaden²,

Saranath³

et al. 2018

IJERPH

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In the current study, ceftazidime- and ciprofloxacin-resistant—or dual drug-resistant (DDR)—E. coli were isolated from river Mula-Mutha, which flows through rural Pune district and Pune city. The DDR E. coli were further examined for antibiotic resistance to six additional antibiotics. The study also included detection of genes responsible for ceftazidime and ciprofloxacin resistance and vectors for horizontal gene transfer. Twenty-eight percent of the identified DDR E. coli were resistant to more than six antibiotics, with 12% being resistant to all eight antibiotics tested. Quinolone resistance was determined through the detection of qnrA, qnrB, qnrS and oqxA genes, whereas cephalosporin resistance was confirmed through detection of TEM, CTX-M-15, CTX-M-27 and SHV genes. Out of 219 DDR E. coli, 8.2% were qnrS positive and 0.4% were qnrB positive. Percentage of isolates positive for the TEM, CTX-M-15 and CTX-M-27 genes were 32%, 46% and 0.9%, respectively. None of the DDR E. coli tested carried the qnrA, SHV and oqxA genes. Percentage of DDR E. coli carrying Class 1 and 2 integrons (mobile genetic elements) were 47% and 8%, respectively. The results showed that antibiotic resistance genes (ARGs) and integrons were present in the E. coli isolated from the river at points adjoining and downstream of Pune city.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India

Dhawde

Macaden²,

Saranath³

et al. 2018

IJERPH

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“…1b). The Kirby-Bauer disk diffusion assay (Bauer et al, 1966) is used in both clinical and research settings (Felmingham and Brown, 2001) and has been widely used to characterize antibiotic resistance for environmental isolates (Sayah et al, 2005;Kumar et al, 2013;Zhang et al, 2014). Both EUCAST and CLSI have standardized protocols for disk diffusion methods.…”

Section: Agar Disk Diffusionmentioning

confidence: 99%

Culture-based Methods for Detection of Antibiotic Resistance in Agroecosystems: Advantages, Challenges, and Gaps in Knowledge

McLain¹,

Cytryn

Durso

et al. 2016

J. Environ. Qual.

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Various culture-based methodologies are used in assessment of antibiotic resistance in samples collected in agroecosystems. Culture-based methods commonly involve isolating target bacteria on general or selective media and assessing growth in response to specific concentrations of antibiotics. The advantages of culture-based methods are multifold. In particular, isolation of bacteria is key to understanding phenotypic characteristics of isolates and their resistance patterns, and most national and international antibiotic resistance monitoring projects are isolate based. This review covers current knowledge of bacterial groups and antibiotics commonly targeted in resistance studies using bacterial culture and discusses the range in methods used, data interpretation, and factors supporting and confounding the use of culture-based methods in assessment of antibiotic resistance. Gaps in knowledge related to study design and resistance databases are discussed. Finally, a case is made for the integration of culture-based and molecular methods to better inform our understanding of antibiotic resistance in agroecosystems.Culture-based Methods for Detection of Antibiotic Resistance in Agroecosystems: Advantages, Challenges, and Gaps in Knowledge Jean E. McLain,* Eddie Cytryn, Lisa M. Durso, and Suzanne Young M ultiple culture-based methods are used to assess antibiotic resistance in environmental samples. Culture-based methods commonly involve isolating target bacteria on general or selective media followed by assessing growth in response to specific concentrations of antibiotics. Target bacteria are commonly pathogens of clinical or public health concern (e.g., Campylobacter, Salmonella) or easily detected bacterial indicators that signify the presence of fecal contamination (e.g., Escherichia coli, total coliforms) (Davies and Davies, 2010). In addition, target bacteria often include those that have the propensity to acquire resistance genes horizontally and to develop resistance to a broad spectrum of last-resort antibiotics (e.g., methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus) (Poole, 2002). Although highthroughput methods are increasing processing capabilities, data production using culture-based methods can be time consuming. Nevertheless, the value of these data cannot be underestimated. Isolation of bacteria is key to understanding and studying phenotypic and genotypic characteristics of individual isolates. Although identification of resistance genes in bulk DNA samples is possible using modern molecular methods, culture and isolation of individual target pathogens carrying the gene of interest is essential for determining antibiotic resistance phenotypes and is therefore integral to national and international antibiotic resistance surveillance and tracking efforts. As such, culture-based studies provide an important link between antibiotic resistance measured in the environment and antibiotic resistance detected in human clinical settings. Microbial Classifications for ...

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“…Enterobacter has a certain drug-resistance, 41 and further research was found that Enterobacter from drinking and recreational water sources exhibited varying antibiotic sensitivity. 42 Correspondingly, one Enterobacter strain was isolated with TC as the sole carbon substrate from the anode at the end of our experiment (Fig. S1 †).…”

Section: Bacterial Community Compositionmentioning

confidence: 96%

Simultaneous degradation of tetracycline by a microbial fuel cell and its toxicity evaluation by zebrafish

Zhang

et al. 2017

RSC Adv.

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Tetracycline (TC) is the second most commonly used antibiotic despite its high toxicity and persistence. In this study, a new approach for the anaerobic biodegradation of TC in a microbial fuel cell (MFC), with glucose-TC mixtures as the substrate, under gradient acclimation conditions was explored. Within 7 days, approximately 79.1% of TC was degraded by the MFC. This value was higher than that obtained through a traditional anaerobic method (14.9%). The TC degradation rates in MFCs with a closed circuit were 31.6% higher than those in MFCs with an open circuit. Furthermore, zebrafish assessment showed that no toxicity was observed after MFC treatment. Microbial community analysis was performed on the anode of the MFCs under gradient acclimation conditions, and the results showed that TC was effectively degraded by the synergy of fermentative bacteria, acid-producing bacteria and electrogenic bacteria. This work confirmed that the anaerobic biodegradation of TC by MFCs is a cost-effective and environmentally friendly method.

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Antibiotic resistance and genetic diversity in water-borne Enterobacteriaceae isolates from recreational and drinking water sources

Cited by 38 publications

References 38 publications

Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India

Antibiotic Resistance Characterization of Environmental E. coli Isolated from River Mula-Mutha, Pune District, India

Culture-based Methods for Detection of Antibiotic Resistance in Agroecosystems: Advantages, Challenges, and Gaps in Knowledge

Simultaneous degradation of tetracycline by a microbial fuel cell and its toxicity evaluation by zebrafish

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