Antibiotic profiling of Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) isolates in stray canines and felines

Kanagarajah, Rajwin Raja; Lee, David Charles Weerasingam; Lee, Daniel Zhi Fung; Yusoff, Khatijah; Paramasivam, Sharmini Julita; Low, Wai Yee; Jeevaratnam, Kamalan; Lim, Swee Hua Erin

doi:10.1080/23312025.2017.1412280

“…Murphy et al, (2009) reported the prevalence of antimicrobial resistance in E. coli was as follows: streptomycin (2%), ampicillin (4%), cephalothrin (1%), and tetracycline (2%). Kanagarajah et al, (2017) reported that feline MRSA isolates resistance were as follows, methicillin (100%), ceftazidime (100%), enrofloxacin (92.31%), oxacillin (84.62%) and vancomycin (0%), 84.62% of feline MRSA isolates indicated resistance to four out of five antibiotics tested. Lazou et al, (2017) reported that all Campylobacter isolates exhibited susceptibility to erythromycin, gentamicin and streptomycin.…”

Section: In-vitro Antibiotic Sensitivity Testmentioning

confidence: 99%

Prevalence of Bacteriological Isolates Recovered from Faecal Sample of Domestic Cats

Patel¹,

Raval²,

Bhanderi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

0

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“…In Malaysia, few studies were conducted to explore the roles of pet dogs and cats as reservoirs of MRSA and MRSP [8][9][10][11]. However, no Malaysian studies focused on detecting MRSP from healthy dogs, cats, or pet owners, as well as performing molecular characterization of MRSP isolates.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Afshar

¹

,

Zakaria

²

,

Chen

³

et al. 2023

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Background and Aim: Staphylococcus aureus and Staphylococcus pseudintermedius are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling. Materials and Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing. Results: One S. aureus isolate and three S. pseudintermedius isolates were identified as MRSA and MRSP, respectively, of which the MRSA isolate and one of the MRSP isolates showed multidrug resistance and the remaining two MRSP isolates were resistant to one or two antimicrobials. Multilocus sequence typing showed that the MRSA isolate belongs to clonal complex (CC) 789, while for the MRSP isolates, two were in CC45 and one was a singleton. Conclusion: This study is the first study in Malaysia to perform molecular characterization of MRSP isolated from pet dogs and cats and pet owners. The outcomes of this study reveal that even healthy pet dogs and cats and their owners can be carriers of drug-resistant staphylococci, highlighting the role of pets and pet owners as carriers of MRSA and MRSP in Malaysia. Keywords: antimicrobial resistance, antimicrobial susceptibility testing, methicillin resistance, multilocus sequence typing, public health, zoonotic infections.

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Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Afshar

¹

,

Zakaria

²

,

Chen

³

et al. 2023

Vet World

0

View full text Add to dashboard Cite

Background and Aim: Staphylococcus aureus and Staphylococcus pseudintermedius are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling. Materials and Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing. Results: One S. aureus isolate and three S. pseudintermedius isolates were identified as MRSA and MRSP, respectively, of which the MRSA isolate and one of the MRSP isolates showed multidrug resistance and the remaining two MRSP isolates were resistant to one or two antimicrobials. Multilocus sequence typing showed that the MRSA isolate belongs to clonal complex (CC) 789, while for the MRSP isolates, two were in CC45 and one was a singleton. Conclusion: This study is the first study in Malaysia to perform molecular characterization of MRSP isolated from pet dogs and cats and pet owners. The outcomes of this study reveal that even healthy pet dogs and cats and their owners can be carriers of drug-resistant staphylococci, highlighting the role of pets and pet owners as carriers of MRSA and MRSP in Malaysia. Keywords: antimicrobial resistance, antimicrobial susceptibility testing, methicillin resistance, multilocus sequence typing, public health, zoonotic infections.

show abstract

Antibiotic profiling of Methicillin Resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines

Cited by 5 publications

References 24 publications

Prevalence of Bacteriological Isolates Recovered from Faecal Sample of Domestic Cats

Prevalence of Bacteriological Isolates Recovered from Faecal Sample of Domestic Cats

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

Prevalence and multidrug-resistant profile of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in dogs, cats, and pet owners in Malaysia

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