Antibiotic-producing bacteria isolated from some natural habitats in the Federal Capital Territory (FCT), Nigeria

Abstract

High global prevalence of multidrug-resistant bacteria due to antibiotics misuse has prompted the need for novel antibacterial compounds to replace the failing antibiotics. This study investigated some natural habitats in Abuja, Nigeria, for antibiotics-producing bacteria. Thirty-six soil samples from termite mounds, river banks and rhizospheres of Anacardium occidentale L. (cashew tree), Gmelina arborea Roxb. ex Sm. (beechwood), Ageratum conyzoides L. (goat weed) including Cymbopogon citratus (DC) Stapf. (lemon grass) were cultured on nutrient media. Twelve potential antibiotic-producing isolates were identified by crowded plates method and characterized using Bergey's manual. The antimicrobial activities of the filtrates from the isolates against some pathogenic strains namely Streptococcus pneumonia, Salmonella typhi, Escherichia coli (ATCC 25922), Proteus mirabilis, Staphylococcus aureus (ATCC 25923), Candida albicans, Bacillus subtilis and Pseudomonas aeruginosa (ATCC 27853) were determined by agar-well diffusion method. Only 5 eventually inhibited at least 1 test microorganism; 4 showed activities against both Gram-positive and Gram-negative bacteria (broad spectrum) and 2 among the 4 also inhibited Candida albicans, while the remaining 1 inhibited only 1 Gram-positive bacterium (narrow spectrum). The 5 potent antibiotics-producers were Bacillus spp. In conclusion, some natural habitats in the FCT are important sources of antibiotic-producing bacteria. Their antimicrobial lead compounds could be extracted and developed locally for pharmaceutical applications.

Impact of Diameter of Inhibition in Crowded Plates on the in vitro Inhibitory Strength of Potential Antibiotic-Producing Bacteria Isolated from Some Natural Habitats

“…A total of 12 potential antibiotic-producing bacteria were isolated from natural habitats by the CPT (Balogun et al, [17]. Fig.…”

“…The five potential candidate strains, AAH02, AMR06/01, AMP03/02, AMP03/05 and ASS02/01, were molecularly identified as Bacillus licheniformis strain SCDB 34, Bacillus lentus strain NCTC4824, Bacillus cereus strain A1, Bacillus brevis strain NCTC2611, and Bacillus subtilis subsp. subtilis 168, respectively [17]. Table 1 illustrates Bacillus subtilis subsp.…”

Antimicrobial and anticancer potential of soil bacterial metabolites - a comprehensive and updated review

“…Members of the genus Bacillus are commonly found in soil and produce a variety of bioactive secondary metabolites that are effective against a variety of life-threatening diseases [42]. As per literature, many bacterial strains isolated from soil samples, for example, Bacillus pumillus, Bacillus lentus, Enterobacter aerogenes, Bacillus alvei, Micrococcus roseus, and Bacillus amyloliquefaciens have shown strong bioactivity against the various pathogens [48][49][50]. Nowadays, most of the research workers focused on soil microbial communities and diversity of soil bacteria especially from Bacillus communities [51].…”