Antibiotic-Induced Alterations in the Osmotic Resistance of Erythrocytes is Modulated by β-Carotene and L –Ascorbic Acid

Luqman, Suaib; Prabu, K.; Pal, Anirban; Saikia, Dharmendra; Darokar, Mahendra P.; Khanuja, S. P. S.

doi:10.1177/1934578x0600100610

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…A stock solution of extract (100 mg/ml) and four flavones (10 mg/ml) was prepared in dimethyl sulphoxide (DMSO), diluted in phosphate buffer saline (PBS) as required. Concentration-dependent studies were carried out (1, 10, 25 and 50 μg/ml) in replicates and osmotic fragility test 23 was performed at 10 and 100 μg/ml.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Density Functional Theory-Based Quantum Rationalization of Flavones From <i>Oroxylum indicum</i>, their Correlation with Redox Effect, Molecular Interaction Studies and Osmotic Hemolysis

et al. 2018

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Four flavones (chrysin, baicalein, oroxylin A and hispidulin) characterized from ethanolic root extract of Oroxylum indicum (a traditional dietary nutraceutical supplement), were compared with both experimental (radical scavenging action and osmotic fragility test on human erythrocytes) and theoretical (Density functional theory (DFT) (B3LYP/6-31G*) calculations and in silico docking with haemoglobin and albumin) for their redox properties. Raman spectra were examined specifically between 2900 and 3700 cm -1 and the corresponding peaks were identified for hydroxyl group stretching vibrations. Baicalein and hispidulin had the highest and lowest binding energy respectively for oxyhaemoglobin (oxyHb) and vice versa for deoxyhaemoglobin (deoxyHb), which was one of the major findings revealed in their superposed docked structures where the position of baicalein was not changed unlike hispidulin. On the whole, baicalein is the preeminent flavone as it revealed maximum activity in various antioxidant assays, protection against osmotic fragility and binding energy with oxyHb which can be reasoned out by its least HOMO-LUMO energy gap.

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Section: Biochemical Assaysmentioning

confidence: 99%

Density Functional Theory-Based Quantum Rationalization of Flavones From <i>Oroxylum indicum</i>, their Correlation with Redox Effect, Molecular Interaction Studies and Osmotic Hemolysis

et al. 2018

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Synthesis of chalcone derivatives on steroidal framework and their anticancer activities☆

et al. 2007

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Suppression of Molecular Targets and Antiproliferative Effect of Citronellal on Triple-Negative Breast Cancer Cells

Fatima

Luqman

2021

CMP

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Background: Triple-negative breast cancer (TNBC) requires targeted therapies to better manage and prevent metastatic mammary gland tumors. Due to the resistance problem associated with the approved drugs, researchers are now focusing on phytochemicals for the treatment of TNBC as they possess a pleiotropic mode of action and fewer side effects. Objective: To investigate the antiproliferative effect of citronellal in triple negative breast cancer cells. Method: Anticancer potential of citronellal was explored by employing SRB, MTT and NRU antiproliferative assay. Further, the effect of citronellal was observed on molecular targets (Tubulin, COX-2 and LOX-5) utilizing in vitro and in silico methods. Furthermore, the efficacy of citronellal was examined on Ehrlich Ascites Carcinoma. In addition, the safety profiling of it was observed at 300 and 1000 mg/kg of body weight in mice. Results: Citronellal suppresses the growth of MDA-MB-231 cells by more than 50% in NRU assay and ~41% and 32% in SRB and MTT assay, respectively. Further, citronellal's effect was observed on molecular targets wherein it suppressed LOX-5 activity (IC50 40.63±2.27 µM) and prevented polymerization of microtubule (IC50 63.62 µM). The result was more prominent against LOX-5 as supported by molecular docking interaction studies, but a non-significant effect was observed at the transcriptional level. The efficacy of citronellal was also determined in Ehrlich Ascites Carcinoma (EAC) model, wherein it inhibited the growth of tumor cells (45.97%) at 75 mg/kg of body weight. It was non-toxic upto 1000 mg/kg of body weight in mice and did not cause significant lysis of erythrocytes. Conclusion: These observations could provide experimental support for citronellal to be used as a chemopreventive agent for breast cancer.

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Antibiotic-Induced Alterations in the Osmotic Resistance of Erythrocytes is Modulated by β-Carotene and L –Ascorbic Acid

Cited by 4 publications

References 23 publications

Density Functional Theory-Based Quantum Rationalization of Flavones From <i>Oroxylum indicum</i>, their Correlation with Redox Effect, Molecular Interaction Studies and Osmotic Hemolysis

Density Functional Theory-Based Quantum Rationalization of Flavones From <i>Oroxylum indicum</i>, their Correlation with Redox Effect, Molecular Interaction Studies and Osmotic Hemolysis

Synthesis of chalcone derivatives on steroidal framework and their anticancer activities☆

Suppression of Molecular Targets and Antiproliferative Effect of Citronellal on Triple-Negative Breast Cancer Cells

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