Antibiotic bacitracin induces hydrolytic degradation of nucleic acids

Ciesiołka, Jerzy; Jeżowska‐Bojczuk, Małgorzata; Wrzesiński, Jan; Stokowa-Sołtys, Kamila; Nagaj, Justyna; Kasprowicz, Aleksandra; Błaszczyk, Leszek; Szczepanik, Wojciech

doi:10.1016/j.bbagen.2014.01.034

Cited by 20 publications

(15 citation statements)

References 30 publications

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“…As shown in Table 3, BA solution showed antibacterial activities against only gram-positive bacteria -a result consistent with previous findings. 6,[23][24][25][26] Nano-BAs (Nano-BA 3K and Nano-BA 5K ) had high effectiveness against both gram-positive and gram-negative bacteria. Their MICs were cell-type dependent and were close to or even below the CMC of the triblock copolymers.…”

Section: Antibacterial Activities Of the Nano-basmentioning

confidence: 99%

“…DMSO-d 6 and CDCl 3 were used as the solvent, including 0.03 vol.% tetramethylsilane (TMS). The composition and block length of PLGA were calculated by comparing the proton-peak integration of LA and GA from 1 H-NMR spectra.…”

Section: Synthesis Of Ba-b-plga-b-plgamentioning

confidence: 99%

“…5 In addition, BA disintegrates the bacterial cytoplasmic membrane, thus leading to the loss of various ions and amino acids and resulting in the death of bacteria. [6][7][8] However, BA has a complex chemical structure and high molecular weight, and it is unable to pass through porins in the outer membrane of gram-negative bacteria to reach the cell wall area, which is their site of action. Therefore, gram-negative bacteria are intrinsically resistant to BA.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo

Hong

Gao

Qiu

et al. 2017

IJN

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Bacitracin A (BA) is an excellent polypeptide antibiotic that is active against gram-positive bacteria without triggering multidrug resistance. However, BA is inactive against gram-negative bacteria because of its inability to cross the outer membrane of these cells, and it has strong nephrotoxicity, thus limiting its clinical applications. Nanoantibiotics can effectively localize antibiotics to the periplasmic space of bacteria while decreasing the adverse effects of antibiotics. In this study, biodegradable hydrophobic copolymers of poly ( d , l -lactide-co-glycolide) (PLGA) were attached to the N-termini of BA to design a novel class of self-assembled nano-bacitracin A (nano-BAs), and their potential as antibacterial agents was evaluated in vitro and in vivo. Nano-BAs had a core-shell structure with a mean diameter <150 nm. Impressively, nano-BAs had strong antibacterial properties against both gram-positive and gram-negative bacteria, and the distribution of antibacterial activity as a function of PLGA block length was skewed toward longer PLGA chains. No cytotoxicity against HK-2 cells or human red blood cells (hRBCs) was observed in vitro, suggesting good biocompatibility. A high local density of BA mass on the surface promoted endocytotic cellular uptake, and hydrophobic interactions between the PLGA block and lipopolysaccharide (LPS) facilitated the uptake of nano-BAs, thereby leading to greater antibacterial activities. In addition, Nano-BA 5K was found to be effective in vivo, and it served as an anti-infective agent for wound healing. Collectively, this study provides a cost-effective means of developing self-assembling nano-polypeptide antibiotic candidates with a broader antibacterial spectrum and a lower toxicity than commercially available peptide antibiotics, owing to their modification with biodegradable copolymers.

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Section: Antibacterial Activities Of the Nano-basmentioning

confidence: 99%

Section: Synthesis Of Ba-b-plga-b-plgamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo

Hong

Gao

Qiu

et al. 2017

IJN

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“…É amplamente utilizada em produtos farmacêuticos de administração tópica, além de poder ser utilizada em aplicações orais para esterilização do trato gastrointestinal e tratamento de algumas infecções gastrointestinais, limitadas aplicações intramusculares e em produtos veterinários. É frequentemente encontrada em produtos farmacêuticos em associação com outros antibióticos, como a neomicina e a polimixina, e raramente produz hipersensibilidade (MING & EPPERSON, 2002;SARRI et al, 2006;BRUNTON et al, 2006;POTTS et al, 2012;CIESIOLKA et al, 2014). (CIESIOLKA et al, 2014).…”

Section: Bacitracina Zincicaunclassified

“…É frequentemente encontrada em produtos farmacêuticos em associação com outros antibióticos, como a neomicina e a polimixina, e raramente produz hipersensibilidade (MING & EPPERSON, 2002;SARRI et al, 2006;BRUNTON et al, 2006;POTTS et al, 2012;CIESIOLKA et al, 2014). (CIESIOLKA et al, 2014). …”

Section: Bacitracina Zincicaunclassified

Doseamento microbiológico de neomicina e bacitracina - desenvolvimento e validação de método rápido em microplacas

Francisco¹

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Antimicrobial effect of Bacillus licheniformis HN-5 bacitracin A on rice pathogen Pantoea ananatis

et al. 2020

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Antibiotic bacitracin induces hydrolytic degradation of nucleic acids

Cited by 20 publications

References 30 publications

Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo

Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo

Doseamento microbiológico de neomicina e bacitracina - desenvolvimento e validação de método rápido em microplacas

Antimicrobial effect of Bacillus licheniformis HN-5 bacitracin A on rice pathogen Pantoea ananatis

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