Antibiotic Activity of Wickerhamomyces anomalus Mycocins on Multidrug-Resistant Acinetobacter baumannii

Junges, Daniele Schaab Boff; Delabeneta, Mateus F; Rosseto, Lana Rubia B; Nascimento, Bruna Larissa; Paris, A.; Persel, Cristiane; Loth, Eduardo Alexandre; Simão, Rita de Cássia Garcia; Menolli, Rafael Andrade; Paula, Claudete Rodrigues; Gandra, Rinaldo Ferreira

doi:10.1007/s00248-020-01495-9

Cited by 14 publications

(13 citation statements)

References 37 publications

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“…Nascimento et al, (2020) reported that mycocins have an antimicrobial action, and are minimally toxic to human cells. Junges et al (2020) confirmed this low toxicity of mycocins, by the cytotoxicity tests in Artemia salina Leach.…”

Section: Introductionsupporting

confidence: 64%

Antimicrobial activity of Wickerhamomyces anomalus mycocins against strains of Staphylococcus aureus isolated from meats

et al. 2021

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Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contaminated with S. aureus coagulase positive is considered a public health risk because the bacterium is resistant to several conventional antimicrobials. Mycocins are substances produced by yeasts that secrete glycoproteins, which can also be called toxin killers, they have an inhibitory action on other microorganisms. The Wickerhamomyces anomalus, is one of the microorganisms capable of producing these mycocins, resulting in the action of disturbances on the cell wall of the pathogen causing deleterious effects. This work aims to evaluate the antimicrobial activity of the mycocins produced by W. anomalus WA45 against the 29 strains of S. aureus coagulase positive isolated from bovine, porcine and chicken meat and 1 standard strain. The antimicrobial action of the mycocins present on the culture supernatant of W. anomalus WA45 was tested by microdilution and the results were satisfactory, since 100% inhibition of strains of S. aureus coagulase positive. We concluded that the mycocins present in the supernatant of W. anomalus WA45 showed antimicrobial action, being candidates for the development of new products for the biocontrol and bioconservation of meat.

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Section: Introductionsupporting

confidence: 64%

Antimicrobial activity of Wickerhamomyces anomalus mycocins against strains of Staphylococcus aureus isolated from meats

et al. 2021

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“…In fact, the biochemical characterization of WaF17.12-KT revealed a glycoproteic structure with an exo-β-1,3-glucanase activity that binds to specific receptors on the surface of microbes but does not target mammalian cells [30]. To our knowledge, there are a few studies in vitro or in vivo on the toxicity of W. anomalus and KTs [8,12,13]. Although yeast KTs appear to be harmless to non-microbial targets, it is important to establish that there are no other yeast toxicity mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…The effect on the growth reactivation and the maintenance of the killer toxin activity after freeze-drying was evaluated, demonstrating that WaF17.12 is a stress-tolerant strain that is transformable into a dried product, ready to use in the field. Concerning the safety, despite several killer strains of W. anomalus being used in the food industry [11], there are only a few studies demonstrating that mice orally treated with the yeast did not show collateral effects [12] and KTs did not harm murine hepatocytes and human erythrocytes in vitro [8,13]. Here, we estimated the effect of WaF17.12 in terms of cell viability and the proinflammatory response on human keratinocyte cell lines, which represent the first barrier of the human body.…”

Section: Introductionmentioning

confidence: 99%

Formulation and Safety Tests of a Wickerhamomyces anomalus–Based Product: Potential Use of Killer Toxins of a Mosquito Symbiotic Yeast to Limit Malaria Transmission

Cappelli

Amantini

Maggi

et al. 2021

Toxins

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Wickerhamomyces anomalus strain WaF17.12 is a yeast with an antiplasmodial property based on the production of a killer toxin. For its symbiotic association with Anopheles mosquitoes, it has been proposed for the control of malaria. In an applied view, we evaluated the yeast formulation by freeze-drying WaF17.12. The study was carried out by comparing yeast preparations stored at room temperature for different periods, demonstrating that lyophilization is a useful method to obtain a stable product in terms of cell growth reactivation and maintenance of the killer toxin antimicrobial activity. Moreover, cytotoxic assays on human cells were performed, showing no effects on the cell viability and the proinflammatory response. The post-formulation effectiveness of the killer toxin and the safety tests indicate that WaF17.12 is a promising bioreagent able to impair the malaria parasite in vector mosquitoes.

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“…Other KTs produced by W. anomalus strains isolated from plants, marine environment, milk, or insect microbiota showed to exert direct antimicrobial activity on different yeast species, including azole-resistant clinical isolates of C. albicans and C. glabrata [ 24 , 53 , 56 , 57 ], malaria parasites [ 32 ] and pathogenic bacteria, such as Listeria monocytogenes and multidrug-resistant strains of Acinetobacter baumannii [ 54 , 55 ].…”

Section: Killer Toxins’ Medical Applicationsmentioning

confidence: 99%

Wickerhamomyces Yeast Killer Toxins’ Medical Applications

Giovati

Ciociola

Simone

et al. 2021

Toxins

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Possible implications and applications of the yeast killer phenomenon in the fight against infectious diseases are reviewed, with particular reference to some wide-spectrum killer toxins (KTs) produced by Wickerhamomyces anomalus and other related species. A perspective on the applications of these KTs in the medical field is provided considering (1) a direct use of killer strains, in particular in the symbiotic control of arthropod-borne diseases; (2) a direct use of KTs as experimental therapeutic agents; (3) the production, through the idiotypic network, of immunological derivatives of KTs and their use as potential anti-infective therapeutics. Studies on immunological derivatives of KTs in the context of vaccine development are also described.

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Antibiotic Activity of Wickerhamomyces anomalus Mycocins on Multidrug-Resistant Acinetobacter baumannii

Cited by 14 publications

References 37 publications

Antimicrobial activity of Wickerhamomyces anomalus mycocins against strains of Staphylococcus aureus isolated from meats

Antimicrobial activity of Wickerhamomyces anomalus mycocins against strains of Staphylococcus aureus isolated from meats

Formulation and Safety Tests of a Wickerhamomyces anomalus–Based Product: Potential Use of Killer Toxins of a Mosquito Symbiotic Yeast to Limit Malaria Transmission

Wickerhamomyces Yeast Killer Toxins’ Medical Applications

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