Antibiotic activity and microbial community of the temperate sponge, <i>Haliclona</i>
 sp.

Hoppers, A.; Stoudenmire, Julie L.; Wu, Shuqi; Lopanik, Nicole B.

doi:10.1111/jam.12709

Cited by 16 publications

(9 citation statements)

References 94 publications

(157 reference statements)

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“…Marine sponges (phylum Porifera ) in particular are known to be a rich source of bioactive compounds, many of which are produced by the bacteria which reside within the sponge host (Abdelmohsen et al, 2014; Fuerst, 2014). Many of these bioactives have antimicrobial activities, making these sponge-associated microbial and fungal communities a potentially valuable source of novel antimicrobials (Baker et al, 2009; Flemer et al, 2012; Hoppers et al, 2015; Indraningrat et al, 2016; Jackson et al, 2018). While sponge bacteria-derived antimicrobial compounds have to date been identified from 35 different genera, the most predominant producing genera include Streptomyces , Pseudovibrio and Bacillus strains (Indraningrat et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Marine Sponge-Derived Streptomyces spp. Isolates SM17 and SM18 With Their Closest Terrestrial Relatives Provides Novel Insights Into Environmental Niche Adaptations and Secondary Metabolite Biosynthesis Potential

et al. 2019

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The emergence of antibiotic resistant microorganisms has led to an increased need for the discovery and development of novel antimicrobial compounds. Frequent rediscovery of the same natural products (NPs) continues to decrease the likelihood of the discovery of new compounds from soil bacteria. Thus, efforts have shifted toward investigating microorganisms and their secondary metabolite biosynthesis potential, from diverse niche environments, such as those isolated from marine sponges. Here we investigated at the genomic level two Streptomyces spp. strains, namely SM17 and SM18, isolated from the marine sponge Haliclona simulans , with previously reported antimicrobial activity against clinically relevant pathogens; using single molecule real-time (SMRT) sequencing. We performed a series of comparative genomic analyses on SM17 and SM18 with their closest terrestrial relatives, namely S. albus J1074 and S. pratensis ATCC 33331 respectively; in an effort to provide further insights into potential environmental niche adaptations (ENAs) of marine sponge-associated Streptomyces , and on how these adaptations might be linked to their secondary metabolite biosynthesis potential. Prediction of secondary metabolite biosynthetic gene clusters (smBGCs) indicated that, even though the marine isolates are closely related to their terrestrial counterparts at a genomic level; they potentially produce different compounds. SM17 and SM18 displayed a better ability to grow in high salinity medium when compared to their terrestrial counterparts, and further analysis of their genomes indicated that they possess a pool of 29 potential ENA genes that are absent in S. albus J1074 and S. pratensis ATCC 33331. This ENA gene pool included functional categories of genes that are likely to be related to niche adaptations and which could be grouped based on potential biological functions such as osmotic stress, defense; transcriptional regulation; symbiotic interactions; antimicrobial compound production and resistance; ABC transporters; together with horizontal gene transfer and defense-related features.

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Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Marine Sponge-Derived Streptomyces spp. Isolates SM17 and SM18 With Their Closest Terrestrial Relatives Provides Novel Insights Into Environmental Niche Adaptations and Secondary Metabolite Biosynthesis Potential

et al. 2019

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“…However, little is known regarding the metabolic functions and possible host-microbe interactions of sponge-associated bacteria. Functional roles that have been ascribed to sponge bacteria are carbon fixation (Thacker 2005 ), nitrogen cycling (Hoffmann et al 2009 ; Taylor et al 2007 ) and host defence (Hoppers et al 2014 ; Mollica et al 2012 ; Proksch 1994 ). Host defence is suggested in most cases to be the result of microbe-derived bioactive compounds, and in the last decade, their potential pharmaceutical application has sparked numerous studies (Mehbub et al 2014 ; Indraningrat et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Recovery of Previously Uncultured Bacterial Genera from Three Mediterranean Sponges

et al. 2017

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Sponges often harbour a dense and diverse microbial community. Presently, a large discrepancy exists between the cultivable bacterial fraction from sponges and the community in its natural environment. Here, we aimed to acquire additional insights into cultivability of (previously uncultured) bacteria from three sponge species, namely Aplysina aerophoba, Corticium candelabrum and Petrosia ficiformis, by studying bacterial growth on five media in the form of 60 communities scraped from plates without antibiotics, as well as in the form of individual isolates that were grown on these media supplemented with antibiotics. We applied (double-)barcoded 16S ribosomal RNA (rRNA) gene amplicon sequencing for species identification. We show that previously uncultured bacteria can be cultivated using conventional plating and that application of antibiotics in the media can serve to capture a greater bacterial diversity. Moreover, we present criteria to address an important caveat of the plate scraping method whereby bacteria may be detected that did not actually grow. Fourteen out of 27 cultivated novel taxa (<95% identity of the 16S rRNA gene amplicon to reported species) belong to Actinobacteria, which indicates the presence of a large untapped reservoir of bioactive compounds. Three Flavobacteriaceae spp. were isolated that potentially constitute two new genera and one new species.Electronic supplementary materialThe online version of this article (doi:10.1007/s10126-017-9766-4) contains supplementary material, which is available to authorized users.

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“…Many of these metabolites have been linked with a microbial origin (Haygood et al, 1999;Lozada and Dionisi, 2015). In the case of Haliclona spp., at least 190 secondary metabolites have been identified exhibiting biological activities like antibiotic, antifungal, antimicrobial, antimalarial, cytotoxic activities, among others (Yu et al, 2006;Hoppers et al, 2015). Several of these compounds are produced by their associated microbiota, which make microbiomes the key players in the functional capabilities of sponges (Kennedy et al, 2009;Khan et al, 2011Khan et al, , 2014.…”

Section: Resultsmentioning

confidence: 99%

Microbial Diversity Exploration of Marine Hosts at Serrana Bank, a Coral Atoll of the Seaflower Biosphere Reserve

Álvarez-Yela¹,

Mosquera-Rendón²,

Noreña-P³

et al. 2019

Front. Mar. Sci.

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Microorganisms represent nearly 90% of ocean biomass and are fundamental for the functioning and health of marine ecosystems due to their integral contribution to biogeochemical cycles and biological processes. In marine environments, microorganisms exist as microbial communities in the water column, benthonic substrates, and macroorganisms, where they establish symbiotic interactions and fulfill their ecological roles. Such interactions can have a harmful or beneficial impact on the hosts depending on the emergent properties of the communities, their taxonomic structure, and functionality. To evaluate these features, culture independent approaches like metabarcoding have been developed and have hugely contributed to the characterization of marine microbial diversity. The present study was aimed to explore the structure and metabolic functionality of microbial communities associated to marine hosts at the Serrana Bank, a coral atoll part of the Seaflower Biosphere Reserve (Archipelago of San Andrés, Old Providence and Saint Catalina, Colombia). We found a highly diverse microbial assemblage associated with the corals Siderastrea siderea, Colpophyllia natans, and Orbicella annularis, the sponge Haliclona sp. and sediment from Isla de los Pájaros lagoon. However, the coral Porites astreoides had significantly lower bacterial diversity and a different community composition. Proteobacteria was the most abundant phylum within bacterial communities in the evaluated hosts, except in P. astreoides, where Cyanobacteria was the predominant group. Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria, Chloroflexi, and Gemmatimonadetes were also identified within all microbiomes, but their dominance varied between hosts. Additionally, the most abundant group among the fungi communities associated with O. annularis, S. siderea, and C. natans was Ascomycota, but significant differences between clasess and order were observed among hosts. Finally, functional profiles revealed that the principal microbial functions were focused on membrane transport, carbohydrates, amino acids and energy metabolism, replication, and translation processes. A significant higher metabolic functionality was found in the sponge microbiome in comparison to the coral microbial communities.

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Antibiotic activity and microbial community of the temperate sponge, Haliclona sp.

Abstract: These data suggest that common temperate sponges can be a source of bioactive chemical and microbial diversity. Further studies may reveal the importance of the microbial associates to the sponge and natural product biosynthesis.

Cited by 16 publications

References 94 publications

Comparative Genomics of Marine Sponge-Derived Streptomyces spp. Isolates SM17 and SM18 With Their Closest Terrestrial Relatives Provides Novel Insights Into Environmental Niche Adaptations and Secondary Metabolite Biosynthesis Potential

Comparative Genomics of Marine Sponge-Derived Streptomyces spp. Isolates SM17 and SM18 With Their Closest Terrestrial Relatives Provides Novel Insights Into Environmental Niche Adaptations and Secondary Metabolite Biosynthesis Potential

Recovery of Previously Uncultured Bacterial Genera from Three Mediterranean Sponges

Microbial Diversity Exploration of Marine Hosts at Serrana Bank, a Coral Atoll of the Seaflower Biosphere Reserve

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