Antibiogram and Detection of ESBL Production in Klebsiella species Isolated from Various Clinical Samples

Bhurle, Arati; Devnikar, Anushka V.; Sonth, Suresh B; Solabannavar, Shivakumar

doi:10.20546/ijcmas.2018.706.140

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…The current study finding that all Kp isolates were MDR is consistent with the study of Huynh et al (2020) [ 78 ]. The antibiogram of Kp isolates showed increased resistance toward cephalosporins and fluoroquinolones [ 79 ]. In the present study, hvKp were highly resistant to ciprofloxacin (100%), cephalosporins (70–94%), and aztreonam (70%).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

et al. 2023

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Hypervirulent Klebsiella pneumoniae (hvKp) is emerging worldwide. Hypermucoviscousity is the characteristic trait that distinguishes it from classic K. pneumoniae (cKp), which enables Kp to cause severe invasive infections. This research aimed to investigate the hypermucoviscous Kp (hmvKp) phenotype among gut commensal Kp isolated from healthy individuals and attempted to characterize the genes encoding virulence factors that may regulate the hypermucoviscosity trait. Using the string test, 50 identified Kp isolates from healthy individuals’ stool samples were examined for hypermucoviscosity and investigated by transmission electron microscopy (TEM). Antimicrobial susceptibility profiles of Kp isolates were determined using the Kirby Bauer disc method. Kp isolates were tested for genes encoding different virulence factors by PCR. Biofilm formation was assayed by the microtiter plate method. All Kp isolates were multidrug-resistant (MDR). Phenotypically, 42% of isolates were hmvKp. PCR-based genotypic testing revealed the hmvKp isolates belonged to capsular serotype K2. All study Kp isolates harbored more than one virulence gene. The genes magA and rmpA were not detected, while the terW gene was present in all isolates. The siderophores encoding genes entB and irp2 were most prevalent in hmvKp isolates (90.5%) and non-hmvKp (96.6%), respectively. hmvKp isolates harbored the genes wabG and uge with rates of 90.5% and 85.7%, respectively. The outcomes of this research highlight the potential health risk of commensal Kp to cause severe invasive diseases, owing to being hmvKp and MDR, and harboring multiple virulence genes. The absence of essential genes related to hypermucoviscosity such as magA and rmpA in hmvKp phenotypes suggests the multifactorial complexity of the hypermucoviscosity or hypervirulence traits. Thus, further studies are warranted to verify the hypermucoviscosity-related virulence factors among pathogenic and commensal Kp in different colonization niches.

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Section: Discussionmentioning

confidence: 99%

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

et al. 2023

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“…The antibiogram of Klebsiella isolates studied in India showed higher resistance against cephalosporins and fluoroquinolones, whereas the highest sensitivity was observed toward imipenem. [ 22 ] Higher coresistance to cephalosporins as well as quinolones could be because the plasmids that contain the ESBL gene also contain resistance genes for quinolones. [ 23 ] and availability of these over-the-counter drugs enable people to consume without prescription in incomplete and inappropriate dose regimen.…”

Section: Discussionmentioning

confidence: 99%

Association of Virulence with Antimicrobial Resistance among Klebsiella pneumoniae Isolated from Hospital Settings in Bangladesh

Kawser¹,

Shamsuzzaman²

2022

International Journal of Applied &Amp; Basic Medical Research

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: Introduction: Infections caused by multidrug-resistant (MDR) hypervirulent Klebsiella pneumoniae are difficult to treat and associated with high mortality rates. Hence, this study was conducted to determine the antibiotic resistance pattern along with the distribution of virulence genes among isolated string test positive and negative strains. Materials and Methods: A total of 44 K . pneumoniae strains were isolated following standard microbiological methods from 350 different clinical samples from patients admitted to Dhaka Medical College Hospital, Bangladesh. String test was done to detect the hypermucoid phenotype. Antimicrobial resistance (AMR) pattern was determined by dichlorodiphenyltrichloroethane (except colistin and fosfomycin) among all isolates. Polymerase chain reaction was done to detect the hypervirulence genes ( magA , rmpA , rmpA2 iutA , iroN ). Results: In this study, 21/44 (47.73%) of the isolated K . pneumoniae were string test positive and distribution of the virulence genes except rmpA2 was higher among them. A total of 15/44 (34.09%) of the isolated K . pneumoniae were MDR, 10/44 (22.73%) were extensively drug resistant, 1/44 (2.27%) was pan drug resistant, and 14/44 (31.82%) were colistin resistant. Isolated organisms were highly resistant to third-generation cephalosporins and most sensitive to fosfomycin in this study. Although all the string test positive strains showed higher resistance rates than the string test negative ones toward most of the tested antibiotics, only the differences of resistance rates to amoxiclav and tigecycline among the two phenotypes were statistically significant. Conclusion: Our findings highlight the importance of surveillance of the AMR pattern of hypervirulent K . pneumoniae in clinical samples. Therefore, a response to check the global dissemination of this hypervirulent K. pneumoniae with resistance determinants is urgently needed.

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Antibiogram and Detection of ESBL Production in Klebsiella species Isolated from Various Clinical Samples

Cited by 2 publications

References 5 publications

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

Association of Virulence with Antimicrobial Resistance among Klebsiella pneumoniae Isolated from Hospital Settings in Bangladesh

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