Antibiofilm polysaccharides

Rendueles, Olaya; Kaplan, Jeffrey B.; Ghigo, Jean‐Marc

doi:10.1111/j.1462-2920.2012.02810.x

Cited by 231 publications

(178 citation statements)

References 99 publications

(153 reference statements)

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“…Our findings suggest that biofilms possess an endogenous structure that defends against invading planktonic competitors regardless of their strain or species identity. This ecological view of biofilm behavior is supported by mechanistic studies establishing that Bacillus, Burkholderia, Escherichia, Pseudomonas, Streptococcus and Vibrio species produce polysaccharides during biofilm formation that reduce surface adherence and growth by con-or hetero-specific bacteria (Rendueles et al, 2013;Anderson et al, 2014).…”

Section: Discussionmentioning

confidence: 82%

“…These biofilm properties, in turn, will have ramifications on the evolution of bacterial behavior, for example, investment into local competition versus dispersal (Hanski et al, 2011), and potentially for the development of novel probiotic strategies for enhancing or inhibiting biofilms (Preidis and Versalovic, 2009;Rendueles et al, 2013). Invasion dynamics will also influence the evolutionary stability of extracellular matrix production (Xavier and Foster, 2007;van Gestel et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Extracellular matrix structure governs invasion resistance in bacterial biofilms

et al. 2015

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Many bacteria are highly adapted for life in communities, or biofilms. A defining feature of biofilms is the production of extracellular matrix that binds cells together. The biofilm matrix provides numerous fitness benefits, including protection from environmental stresses and enhanced nutrient availability. Here we investigate defense against biofilm invasion using the model bacterium Vibrio cholerae. We demonstrate that immotile cells, including those identical to the biofilm resident strain, are completely excluded from entry into resident biofilms. Motile cells can colonize and grow on the biofilm exterior, but are readily removed by shear forces. Protection from invasion into the biofilm interior is mediated by the secreted protein RbmA, which binds mother-daughter cell pairs to each other and to polysaccharide components of the matrix. RbmA, and the invasion protection it confers, strongly localize to the cell lineages that produce it.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Extracellular matrix structure governs invasion resistance in bacterial biofilms

et al. 2015

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“…Microbes defective in polysaccharide production can have increased sensitivity to antimicrobials and to the host immune system (Rendueles et al, 2013). In addition to their protective role, polysaccharides are involved in mediating cell surface and cell-to-cell interactions, and can even act as antibiofilm agents by inhibiting growth of other species (Kostakioti et al, 2013;Rendueles et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

et al. 2014

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Klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. In this work, we carried out an analysis of the genes in the K. pneumoniae yfiRNB operon, previously implicated in biofilm formation. The results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formation due to changes in cellulose as part of the extracellular matrix. Deletion of yfiR resulted in enhanced biofilm formation and an altered colony phenotype indicative of cellulose overproduction when grown on solid indicator media. Extraction of polysaccharides and treatment with cellulase were consistent with the presence of cellulose in biofilms. The enhanced cellulose production did not, however, correlate with virulence as assessed using a Caenorhabditis elegans assay. In addition, cells bearing mutations in genes of the yfiRNB operon varied with respect to the WT control in terms of susceptibility to the antibiotics amikacin, ciprofloxacin, imipenem and meropenem. These results indicated that the yfiRNB operon is implicated in the production of exopolysaccharides that alter cell surface characteristics and the capacity to form biofilms -a phenotype that does not necessarily correlate with properties related with survival, such as resistance to antibiotics.

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“…These and other considerations have generated an increased interest in the development of non-biocidal anti-infective strategies as alternatives to antibiotics, as these would be expected to show reduced tendency to provoke the appearance of resistant strains. [6][7][8][9][10][11][12][13] One such approach is the development of a † Electronic supplementary information (ESI) available. See DOI: 10.1039/ c4nr05906a ‡ These authors contributed equally to the work.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles

et al. 2015

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Inhibition of type 1 fi mbriae-mediated Escherichia coli adhesion and biofi lm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles Trimeric thiosugar clusters, conveniently obtained through a thiol-ene "click" strategy, have been effi ciently conjugated to alkynyl-functionalized nanodiamonds (NDs) using a Cu(I)-catalysed "click" reaction. These tri-thiomannoside clusterconjugated NDs (ND-Man 3 ) are shown to be potent inhibitors of type 1 fi mbriae-mediated E. coli adhesion to yeast and T24 bladder cells and moreover to inhibit E. coli-mediated biofi lm formation. This latter feature has only rarely been reported in the past for analogues featuring such simple multivalent glycosidic motifs and would constitute a useful additional characteristic of any anti-adhesive drug lead. anti-adhesive nanoparticles displaying activity against biofilms. In this work, trimeric thiomannoside clusters conjugated to nanodiamond particles (ND) were targeted for investigation. NDs have attracted attention as a biocompatible nanomaterial and we were curious to see whether the high mannose glycotope density obtained upon grouping monosaccharide units in triads might lead to the corresponding NDconjugates behaving as effective inhibitors of E. coli type 1 fimbriae-mediated adhesion as well as of biofilm formation. The required trimeric thiosugar clusters were obtained through a convenient thiol-ene "click" strategy and were subsequently conjugated to alkynyl-functionalized NDs using a Cu(I)-catalysed "click" reaction. We demonstrated that the tri-thiomannoside cluster-conjugated NDs (ND-Man 3 ) show potent inhibition of type 1 fimbriae-mediated E. coli adhesion to yeast and T24 bladder cells as well as of biofilm formation. The biofilm disrupting effects demonstrated here have only rarely been reported in the past for analogues featuring such simple glycosidic motifs. Moreover, the finding that the tri-thiomannoside cluster (Man 3 N 3 ) is itself a relatively efficient inhibitor, even when not conjugated to any ND edifice, suggests that alternative mono-or multivalent sugar-derived analogues might also be usefully explored for E. coli-mediated biofilm disrupting properties.

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Antibiofilm polysaccharides

Cited by 231 publications

References 99 publications

Extracellular matrix structure governs invasion resistance in bacterial biofilms

Extracellular matrix structure governs invasion resistance in bacterial biofilms

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles

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