Antibiofilm effect of Nocardiopsis sp. GRG 1 (KT235640) compound against biofilm forming Gram negative bacteria on UTIs

Rajivgandhi, Govindan; Vijayan, Ramachandran; Maruthupandy, Muthuchamy; Vaseeharan, Baskaralingam; Manoharan, Natesan

doi:10.1016/j.micpath.2018.03.011

Cited by 62 publications

(29 citation statements)

References 37 publications

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“…27 The phenotypic confirmatory test for ESBL was carried on by combination disk method using cefotaxime (30 µg) and ceftazidime (30 µg) alone and in combination with clavulanic acid (10 µg). 28 , 29 ESBL activities were identified by ≥5 mm increase in zone diameter around the disk with the antibiotic combined with clavulanic acid. 30 , 31 In brief, Muller Hinton agar plates were swabbed with 0.5 mL of overnight bacterial culture; after 10 minutes the antibiotic disk was placed on the plates and incubated for 24.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Hypervirulent Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Among Urinary Tract Infections: The First Report from Iran

Taraghian¹,

Esfahani²,

Moghim³

et al. 2020

IDR

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Introduction This study was conducted to identify the hypermucoviscosity, iron acquisition, and capsule serotypes of K. pneumoniae strains isolated from urinary tract infections among community-acquired patients (CA) and assess the frequency of plasmid-mediated quinolone resistance (PMQR) and extended-spectrum beta-lactamases (ESBL) genes between classic and hypervirulent strains. Materials and Methods A total of 105 K. pneumoniae were isolated from CA-UTI. Demographic data related to the underlying diseases and clinical manifestations were further collected. Antibiotic resistance pattern and molecular characterization were compared among ESBL-positive, ESBL-negative, hypervirulent, and classic isolates. Results The results revealed that 52.4% of the isolates were confirmed as ESBL producers and 11 (10.5%) were considered as hypervirulent K. pneumoniae (hvKp). Ciprofloxacin and nalidixic acid were the most inactive antibiotics with resistance rates of 68.6% and 64.8%, respectively. Molecular characterization revealed that 7.6% of all the isolates carried k1 and 66.6% carried K2 genes. The most frequent ESBL gene was bla SHV 63.8%, followed by bla TEM 59.0%, and bla CTX-M 58.1%. ESBL genes were signiﬁcantly more in hvKp than in cKp. Moreover, 61 (84.7%), 47 (65.2%), and 16 (22.2%) of isolates harbored qnrB, qnrS , and qnrA . ESBL genes were detected in all hvKps, and bla SHV was observed in 90.9% of hvKp ( P value= 0.048, 95%). Discussion This study reported the high frequency of antimicrobial and multidrug resistance among hvKp isolates. Coexistence of PMQR and ESBL genes in hvkp indicates the necessity to enhance the clinical knowledge and management of hvKp infections.

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Section: Methodsmentioning

confidence: 99%

Characterization of Hypervirulent Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Among Urinary Tract Infections: The First Report from Iran

Taraghian¹,

Esfahani²,

Moghim³

et al. 2020

IDR

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“…The temperature of 0–10 min with 10–90% A, 90–100% B and 100% C at 10–11 min, 11–20 min and 15–25 min were maintained in gradient elution program. The 20 µL column injected volume at 40 °C temperature was used ( Rajivgandhi et al, 2018b ).…”

Section: Methodsmentioning

confidence: 99%

Preparative HPLC fraction of Hibiscus rosa-sinensis essential oil against biofilm forming Klebsiella pneumoniae

Yang

Rajivgandhi

Ramachandran

et al. 2020

Saudi Journal of Biological Sciences

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Recent years Klebsiella pneumoniae ( K. pneumoniae ) biofilm formation (BF) is emerging thread worldwide. For tackling this problem, we have chosen Hibiscus rosa-. pneumoniae. The HPLC purified essential oils (EOs sinensis (H. rosa-sinensis) (HRS) to inhibit the BF K) of H. rosa-sinensis was performed against BF K. pneumoniae and showed concentration dependent biofilm inhibition. At the MBIC of EOs (90 µg/ml), the biofilm inhibition was showed at 92% against selected BF K. Pneumoniae . The biofilm metabolic assay, exopolysaccharide quantification and hydrophobicity index variation results exhibited with 88%, 92% and 89% reduction at 90 μg/mL was observed respectively. In addition, the morphological modification of MBIC treated K. pneumoniae was clearly viewed by scanning electron microscope (SEM). Overall, all the invitro experiments result were confirmed that the MBIC of H. rosa-sinensis EOs was very effective against BF K. pneumonia.

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“…A phytochemical compound, Pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), extracted from endophytic actinomycete Nocardiosis sp., was found to inhibit microbial adherence and biofilm formation. The research group proved the QS inhibitory effect of this compound using the QS biosensor Chromobacterium violaceum (ATCC 12472) (Rajivgandhi et al, 2018 ).…”

Section: Therapeutic Strategiesmentioning

confidence: 99%

Proteus mirabilis Biofilm: Development and Therapeutic Strategies

Wasfi

Hamed

Amer

et al. 2020

Front. Cell. Infect. Microbiol.

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Proteus mirabilis is a Gram negative bacterium that is a frequent cause of catheter-associated urinary tract infections (CAUTIs). Its ability to cause such infections is mostly related to the formation of biofilms on catheter surfaces. In order to form biofilms, P. mirabilis expresses a number of virulence factors. Such factors may include adhesion proteins, quorum sensing molecules, lipopolysaccharides, efflux pumps, and urease enzyme. A unique feature of P. mirabilis biofilms that build up on catheter surfaces is their crystalline nature owing to their ureolytic biomineralization. This leads to catheter encrustation and blockage and, in most cases, is accompanied by urine retention and ascending UTIs. Bacteria embedded in crystalline biofilms become highly resistant to conventional antimicrobials as well as the immune system. Being refractory to antimicrobial treatment, alternative approaches for eradicating P. mirabilis biofilms have been sought by many studies. The current review focuses on the mechanism by which P. mirabilis biofilms are formed, and a state of the art update on preventing biofilm formation and reduction of mature biofilms. These treatment approaches include natural, and synthetic compounds targeting virulence factors and quorum sensing, beside other strategies that include carrier-mediated diffusion of antimicrobials into biofilm matrix. Bacteriophage therapy has also shown successful results in vitro for combating P. mirabilis biofilms either merely through their lytic effect or by acting as facilitators for antimicrobials diffusion.

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Antibiofilm effect of Nocardiopsis sp. GRG 1 (KT235640) compound against biofilm forming Gram negative bacteria on UTIs

Cited by 62 publications

References 37 publications

<p>Characterization of Hypervirulent Extended-Spectrum β-Lactamase-Producing <em>Klebsiella pneumoniae</em> Among Urinary Tract Infections: The First Report from Iran</p>

<p>Characterization of Hypervirulent Extended-Spectrum β-Lactamase-Producing <em>Klebsiella pneumoniae</em> Among Urinary Tract Infections: The First Report from Iran</p>

Preparative HPLC fraction of Hibiscus rosa-sinensis essential oil against biofilm forming Klebsiella pneumoniae

Proteus mirabilis Biofilm: Development and Therapeutic Strategies

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