Antibiofilm coatings based on protein-engineered polymers and antimicrobial peptides for preventing implant-associated infections

Abstract

Protein-engineered polymers can be used as multivalent platforms for AMP tethering on implant surfaces as cytocompatible coatings with strong antibiofilm properties.

Biological and Physiochemical Methods of Biofilm Adhesion Resistance Control of Medical-Context Surface

“…In addition, the antimicrobial peptide can be directly deposited on the surface of the catheter, and the surface-modified antimicrobial catheter maintains a long-lasting antimicrobial activity for 21 days with no toxicity to humans [90]. Recently, Acosta et al [91] used biotechnology to obtain recombinant protein polymers to directly immobilise antimicrobial peptides on the surface of titanium alloys. The antimicrobial paint was found to exhibit excellent antimicrobial activity under dynamic biofilm incubation conditions, with good cytocompatibility and low toxicity to mammalian cells, which is a good medical advantage in the prevention of implant infections.…”

The Best Peptidomimetic Strategies to Undercover Antibacterial Peptides

“…Nevertheless, immobilized AMPs have lower ability to interact with multiple targets [ 171 ]. A possible solution is attachment of the peptides to the substrate via a spacer, which increases the flexibility and enhances the functional conformation of AMPs, thereby ameliorating the overall antimicrobial effect [ 172 ]. Such a strategy is a new horizon in the fabrication of biocoatings.…”

“…Such a strategy is a new horizon in the fabrication of biocoatings. Recently, Acosta et al [ 172 ] proposed an effective antibiofilm coatings based on protein-engineered polymers and antimicrobial peptides for preventing implant-associated infections [ 172 ]. The unstructured GL13K (GKIIKLKASLKLL-NH 2 ) peptide ( Figure 13 ) was hybridized with elastin-like recombinamers (ELRs) and subsequently tethered to commercially pure titanium discs, and their biological response was successfully tested against the biofilm activity of oral microorganisms ( S. gordonii and P. gingivalis ).…”

Protein-Engineered Polymers Functionalized with Antimicrobial Peptides for the Development of Active Surfaces

“…This technology is very powerful, enabling the design of multifunctional protein-based materials by fusing genes encoding for individual protein blocks, and even functional modules, into a single large gene [4]. For instance, the structure of rPPs can be genetically manipulated to incorporate the minimal consensus repeats of silk and elastin into the same molecule [5,6] and to include bioactive domains such as antimicrobial peptides (AMPs) [7][8][9]. The versatility of this approach allows custom design of the structure of rPPs to obtain functional protein materials that can be processed into different active materials such as coatings, films and fiber mats [7][8][9][10][11][12][13].…”