Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2

Kang, Min Jae; Khan, Fazlurrahman; Jo, Du-Min; Oh, DoKyung; Tabassum, Nazia; Kim, Young‐Mog

doi:10.3390/antibiotics11111524

Cited by 22 publications

(12 citation statements)

References 102 publications

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“…The appearance of a wine-red color of the reaction mixture and specific absorption spectra (570 nm) in the UV-vis absorption spectroscopy confirmed the successful synthesis of the Fu–AuNPs, as seen previously in several reports [ 47 , 48 ]. The FTIR spectra show that some characteristic bands are unique to AuNPs and fucoidan, as previously identified in fucoidan-derived AuNPs and fucoidan [ 47 , 49 ].…”

Section: Discussionsupporting

confidence: 87%

“…The spherical shape of the Fu–AuNPs is similar to that of the previously synthesized fucoidan–gold nanoparticles [ 47 , 50 ]. Furthermore, several other gold nanoparticles synthesized using the natural product were discovered to be spherical, as well as non-spherical, in shape [ 47 , 48 , 51 ]. The size variation of nanoparticles synthesized using a natural product has been frequently reported.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

et al. 2023

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The polymicrobial proliferation and development of complex biofilm morphologies by bacterial and fungal pathogens in the host are some of the key factors contributing to the failure of antimicrobial treatments. The polymicrobial interaction of Candida albicans and some bacterial species has been extensively studied in both in vitro and in vivo model systems. Alternative strategies for disrupting polymicrobial interaction and biofilm formation are constantly needed. Among several alternative strategies, the use of nanoparticles synthesized using a natural product in the treatment of microbial infection has been considered a promising approach. The current study aimed to synthesize gold nanoparticles (AuNPs) using a natural product, fucoidan, and to test their efficacy against mono and duo combinations of fungal (Candida albicans) and bacterial (Staphylococcus aureus/Streptococcus mutans) biofilms. Several methods were used to characterize and study Fu–AuNPs, including UV-vis absorption spectroscopy, FTIR, FE-TEM, EDS, DLS, zeta potential, and XRD. The concentration-dependent inhibition of early-stage biofilms and the eradication of mature biofilms of single species of C. albicans, S. aureus, and S. mutans have been observed. Early biofilms of a dual-species combination of C. albicans and S. aureus/S. mutans were also suppressed at an increasing concentration of Fu–AuNPs. Furthermore, Fu–AuNPs significantly eradicated the established mature biofilm of mixed species. The treatment method proposed in this study, which involves the use of marine-bioinspired nanoparticles, is a promising and biocompatible agent for preventing the growth of polymicrobial biofilms of bacterial and fungal pathogens.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

et al. 2023

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“…The identification of metabolites present in the LAB supernatant was accomplished using GC–MS analysis. 26 The GC–MS equipment (Agilent Technologies 7890A-5975C) was used to examine the metabolites in the supernatant. The helium gas was used as the carrier with a 1.0 mL/min flow rate.…”

Section: Methodsmentioning

confidence: 99%

“… 23 , 24 As a result, it would be one of the novel approaches to using LAB as an environmentally friendly method of synthesizing biocompatible NPs that can be used to treat a wide variety of microbial infections. 25 , 26 Thus, in the current study, we sought to isolate and characterize the LAB strains from the Kimchi sample as a biological source for the synthesis of AuNPs and AgNPs. In addition, gas chromatography-mass spectroscopy was used to investigate the identified LAB strain C1 for the production of secondary metabolites to determine their various biological functions.…”

Section: Introductionmentioning

confidence: 99%

“…Nanoparticles’ features, such as small size, large surface area, biocompatibility, and targeting to specific sites, make them more suited for use in various biochemical functions. , Most studies used biologically derived raw materials (from algae, fungi, bacteria, and mammals) to synthesize organic or inorganic nanoparticles to treat infectious and noninfectious diseases due to their various benefits over chemically synthesized nanoparticles. − We synthesize two different kinds of metallic nanoparticles, such as silver (AgNPs) and gold (AuNPs), using microbial-derived products as raw materials, which have several advantages. According to a recent study, probiotics, particularly lactic acid bacteria (LAB), have drawn much interest from the food and pharmaceutical industries because of their many advantages for human health and well-being. − Several studies have shown that LAB provides biological protection against microbial infections. , As a result, it would be one of the novel approaches to using LAB as an environmentally friendly method of synthesizing biocompatible NPs that can be used to treat a wide variety of microbial infections. , Thus, in the current study, we sought to isolate and characterize the LAB strains from the Kimchi sample as a biological source for the synthesis of AuNPs and AgNPs. In addition, gas chromatography-mass spectroscopy was used to investigate the identified LAB strain C1 for the production of secondary metabolites to determine their various biological functions …”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

et al. 2023

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The emergence of antibiotic resistance in microbial pathogens necessitates the development of alternative ways to combat the infections that arise. The current study used nanotechnology as an alternate technique to control virulence characteristics and biofilm development in Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, based on the acceptance and biocompatibility of the probiotic bacteria, we chose a lactic acid bacteria (LAB) for synthesizing two types of metallic nanoparticles (NPs) in this study. Using molecular techniques, the LAB strain C1 was isolated from Kimchi food samples and identified as Lactiplantibacillus sp. strain C1. The prepared supernatant from strain C1 was used to produce gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). C1-AuNPs and C1-AgNPs were characterized physiochemically using a variety of instruments. C1-AuNPs and C1-AgNPs had spherical shapes and sizes of 100.54 ± 14.07 nm (AuNPs) and 129.51 ± 12.31 nm (AgNPs), respectively. C1-AuNPs and C1-AgNPs were discovered to have high zeta potentials of −23.29 ± 1.17 and −30.57 ± 0.29 mV, respectively. These nanoparticles have antibacterial properties against several bacterial pathogens. C1-AuNPs and C1-AgNPs significantly inhibited the initial stage biofilm formation and effectively eradicated established mature biofilms of P. aeruginosa and S. aureus. Furthermore, when P. aeruginosa was treated with sub-MIC levels of C1-AuNPs and C1-AgNPs, their different virulence features were significantly reduced. Both NPs greatly inhibited the hemolytic activity of S. aureus. The inhibition of P. aeruginosa and S. aureus biofilms and virulence features by C1-AuNPs and C1-AgNPs can be regarded as viable therapeutic strategies for preventing infections caused by these bacteria.

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Synthesis of NiO nanoparticles from Paulownia tomentosa plant extracts via a green synthesis method and antibacterial, antibiofilm and cytotoxicity applications

Gürsoy,

Çiçek,

Tekerek

et al. 2024

Applied Organom Chemis

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This study revealed the effective production of nickel oxide nanoparticles (NPs) using chelating agents derived from the leaves of Paulownia tomentosa plants and nickel (II) chloride hexahydrate (NiCl2⋅6H2O) as the precursor. Nickel oxide NPs (NiO NPs) have been successfully prepared by a green synthesis method using extracts of P. tomentosa leaves in water. The morphology and crystallinity of the NPs were investigated via X‐ray diffraction (XRD), scanning electron microscopy (SEM), UV–visible spectroscopy (UV–vis), energy‐dispersive X‐ray analysis (EDX), Fourier transform infrared spectroscopy (FT‐IR) and thermogravimetric analysis (TGA). The formation behaviour of NiO NPs and the microstructural and physical properties of the resulting particles were studied. This study additionally investigated the antimicrobial, antibiofilm and cytotoxic effects of NiO NPs. Microstructurally uniform NiO NPs with a nanometre diameter were consistently produced using an eco‐friendly processing approach and exhibited a visible‐range transparency of 89%. The obtained SEM images confirmed the octahedral shape of the NPs. The NiO NPs acquired were found to have potent antimicrobial efficacy against all the other bacterial strains. As a result of their antibiofilm effects on pathogenic Escherichia coli and Pseudomonas aeruginosa bacteria, NiO NPs strongly inhibited these bacteria in a concentration‐dependent manner. In the cytotoxicity study of NiO NPs, a decrease in cell viability was shown to occur depending on the concentration of the cell lines used.

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Antibiofilm and Antivirulence Activities of Gold and Zinc Oxide Nanoparticles Synthesized from Kimchi-Isolated Leuconostoc sp. Strain C2

Cited by 22 publications

References 102 publications

Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles

Inhibition of Biofilm and Virulence Properties of Pathogenic Bacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus sp. Strain C1

Synthesis of NiO nanoparticles from Paulownia tomentosa plant extracts via a green synthesis method and antibacterial, antibiofilm and cytotoxicity applications

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