Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens

Busetti, Alessandro; Thompson, Thomas P.; Tegazzini, Diana; Megaw, Julianne; Maggs, Christine A.; Gilmore, Brendan

doi:10.3390/md13063581

Cited by 18 publications

(12 citation statements)

References 82 publications

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“…The marine environment facilitates the formation of microbial biofilms on all abiotic and biotic surfaces underwater [ 98 ]. Algae, aquatic organisms carrying out photosynthesis, have to keep their surface free of fouling [ 99 ].…”

Section: Antibiofilm Effects Of Polyphenols From Algaementioning

confidence: 99%

“…Biofilms are microbial communities covered with a common glycocalyx, which is a complex polymeric (polysaccharide) structure. In humans and animals, such biofilms are able to avoid the factors of innate and adaptive immunity [ 98 , 100 ]. They are characterized by an increased rate of horizontal genetic transfer, leading to the acquisition and spread of antibiotic and multidrug resistance.…”

Section: Antibiofilm Effects Of Polyphenols From Algaementioning

confidence: 99%

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Algae Polyphenolic Compounds and Modern Antibacterial Strategies: Current Achievements and Immediate Prospects

et al. 2020

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The increasing drug resistance of pathogenic microorganisms raises concern worldwide and necessitates the search for new natural compounds with antibacterial properties. Marine algae are considered a natural and attractive biotechnological source of novel antibiotics. The high antimicrobial activity of their polyphenolic compounds is a promising basis for designing innovative pharmaceuticals. They can become both a serious alternative to traditional antimicrobial agents and an effective supplement to antibiotic therapy. The present review summarizes the results of numerous studies on polyphenols from algae and the range of biological activities that determine their biomedical significance. The main focus is put on a group of the polyphenolic metabolites referred to as phlorotannins and, particularly, on their structural diversity and mechanisms of antimicrobial effects. Brown algae are an almost inexhaustible resource with a high biotechnological potential for obtaining these polyfunctional compounds. An opinion is expressed that the effectiveness of the antibacterial activity of phlorotannins depends on the methods of their extraction aimed at preserving the phenolic structure. The use of modern analytical tools opens up a broad range of opportunities for studying the metabolic pathways of phlorotannins and identifying their structural and functional relationships. The high antimicrobial activity of phlorotannins against both Gram-positive and Gram-negative bacteria provides a promising framework for creating novel drugs to be used in the treatment and prevention of infectious diseases.

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Section: Antibiofilm Effects Of Polyphenols From Algaementioning

confidence: 99%

Section: Antibiofilm Effects Of Polyphenols From Algaementioning

confidence: 99%

Algae Polyphenolic Compounds and Modern Antibacterial Strategies: Current Achievements and Immediate Prospects

et al. 2020

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“…The bioactive potential of H. siliquosa was identified over four decades ago. Hornsey & Hide (1974, 1976 active extracts showed no toxicity against wax moth (G. mellonella) larvae across a wide range of concentrations up to the highest concentration tested (Busetti et al, 2015). The final antimicrobial activity exhibited by the crude extract using the disc diffusion assay or the MIC assay against MRSA ATCC 33593 is the result of the additive or synergistic activity of two distinct groups of compounds whereas the activity observed against C. violaceum ATCC 12472 results from three distinct groups of compounds.…”

Section: Brown Seaweeds (Phaeophyceae)mentioning

confidence: 98%

“…Hornsey & Hide (1974, 1976 highlighting the potential of this alga for the treatment of mycobacterial and protozoal infections. Active extracts showed no toxicity against wax moth (G. mellonella) larvae across a wide range of concentrations (Busetti et al, 2015) 13) have highlighted the presence of "core microbial species" in mutualistic or obligate association with their host . In particular, several bacterial epiphytes have been reported to produce bioactive compounds that can protect macroalgal surfaces from biofouling (Dobretsov & Qian, 2002).…”

Section: Brown Seaweeds (Phaeophyceae)mentioning

confidence: 99%

Marine macroalgae and their associated microbiomes as a source of antimicrobial chemical diversity

Busetti

Maggs²,

Gilmore

2017

European Journal of Phycology

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Antimicrobial resistance (AMR) represents one of the major health threats faced by humanity over the next few years. To prevent a global epidemic of antimicrobial-resistant infections, the discovery of new antimicrobials and antibiotics, better anti-infection strategies and diagnostics, and changes to our current use of antibiotics have all become of paramount importance. Numerous studies investigating the bioactivities of seaweed extracts as well as their secondary and primary metabolites highlight the vast biochemical diversity of seaweeds, with new modes of action making them ideal sources for the discovery of novel antimicrobial bioactive compounds of pharmaceutical interest. In recent years, researchers have focused on characterizing the endophytic and epiphytic microbiomes of various algal species in an attempt to elucidate host-microbe interactions as well as to understand the function of microbial communities. Although environmental and host-associated factors crucially shape microbial composition, microbial mutualistic and obligate symbionts are often found to play a fundamental role in regulating many aspects of host fitness involving ecophysiology and metabolism. In particular, algal "core" epiphytic bacterial communities play an important role in the protection of surfaces from biofouling, pathogens and grazers through the production of bioactive metabolites. Together, marine macroalgae and their associated microbiomes represent unique biological systems offering great potential for the isolation and identification of novel compounds and strategies to contrast the rise and dissemination of AMR.

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“…Marine environment known to have vast diversity of organisms which produce novel beneficial bioactive compounds. Among them, marine algae received special attention because of its immense chemical diversity that offers a strong target for a concerted global effort in order to discover new antimicrobials to attempt to tackle bacterial infection and the widespread diffusion of antimicrobial resistance (AMR) (Busetti et al ).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algaeChlamydomonas reinhardtii

Vishwakarma

Vavilala

2019

J Appl Microbiol

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Aim of the study In this study, antibacterial and antibiofilm potential of sulphated polysaccharides (SPs) extracted from Chlamydomonas reinhardtii (Cr) was evaluated against Neisseria mucosa, Escherichia coli, Streptococcus sp. and Bacillus subtilis. Methods and Results Antibacterial potential of Cr‐SPs was evaluated by agar‐cup diffusion, time‐kill and colony‐forming ability (CFU), minimum inhibitory and bactericidal concentration assays. Antibiofilm potential was evaluated by biofilm inhibition, eradication, extracellular‐DNA, metabolic activity and microscopy assays. Cr‐SPs at 0·5 mg ml−1 showed 34·52, 48·6, 66·1 and 55·6% reduced CFU in B. subtilis, Streptococcus, N. mucosa and E. coli respectively. Minimum inhibitory concentration of Cr‐SPs was as low as 480 μg ml−1 for Streptococcus, N. mucosa and 420 μg ml−1 for B. subtilis and E. coli. At 1 mg ml−1, Cr‐SPs showed 50% biofilm inhibition, whereas 4–8 mg ml−1 showed 100% inhibition. Cr‐SPs also effectively dissolved preformed biofilms. Dose‐dependent reduction in extracellular DNA revealed that Cr‐SPs interacts with the extra polymeric substance of the biofilm and destroys them. Light microscopy reconfirmed the above results. Conclusion Cr‐SPs not only inhibited biofilm formation but also effectively dissolved preformed‐biofilms. Significance and Impact of the Study The current study showed the promising potential of Cr‐SPs as antibiofilm agents. Further validation will help in developing Cr‐SPs as natural antibiotics against biofilm‐causing bacteria.

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Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens

Cited by 18 publications

References 82 publications

Algae Polyphenolic Compounds and Modern Antibacterial Strategies: Current Achievements and Immediate Prospects

Algae Polyphenolic Compounds and Modern Antibacterial Strategies: Current Achievements and Immediate Prospects

Marine macroalgae and their associated microbiomes as a source of antimicrobial chemical diversity

Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algaeChlamydomonas reinhardtii

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