Antibiofilm activity of Andrographis paniculata against cystic fibrosis clinical isolate Pseudomonas aeruginosa

Abstract

Respiratory tract and device associated infections caused by biofilm forming Pseudomonas aeruginosa play a primary role in the pathogenesis and prognosis of cystic fibrosis (CF) diseases. The biofilm formed by these pathogens attributes to the antibiotic resistance and protection from host immune response. Once established, the pathogens respond poorly to therapeutic agents. Recently medicinal plants are largely explored as potential source of bioactive agents. In this context the present study reports the antibiofilm activity of the folkloric medicinal plant Andrographis paniculata against biofilm forming CF causative Pseudomonas aeruginosa isolated from CF sputum. P. aeruginosa was also assessed for their growth and development of the biofilm, phylogenetic relationship and antibiotic susceptibility. Antibiogram of the strains indicated that they were resistant to more than one antibiotic. Six extracts of A. paniculata showed significant antibiofilm activity. P. aeruginosa strains, KMS P03 and KMS P05, were found to be maximally inhibited by the methanol extract to an extent of 88.6 and 87.5% respectively. This is the first report on antibiofilm activity of A. paniculata extracts, and our results indicate scope for development of complementary medicine for biofilm associated infections.

Anti-biofilm activity ofSalvadora persicaon cariogenic isolates ofStreptococcus mutans:in vitroand molecular docking studies

“…In the present study, the susceptibility of S. mutans to different extracts varied considerably, indicating the presence of more than one bioactive principle. Alcohol was found to be a better solvent for the extraction of antibacterial substances from S. persica active against cariogenic bacteria compared with water and hexane, which corroborates the results obtained by other authors (Ahmad et al 1998;Hwang et al 2004;Murugan et al 2010a) using other plants. Interestingly, the methanol extract was more potent than other extracts.…”

“…These isolates were identified (using Api-20 STREP) according to National Committee for Clinical Laboratory Standards (NCCLS) and maintained in Brain-Heart Infusion (BHI) Agar with 5% (v/v) defibrinated blood (Al-Anbori et al 2008). The slimeforming ability of the isolates was evaluated using the tube adherence method devised by Christensen et al (1982) and graded as described (Murugan et al 2010a). The extracellular polysaccharide substance and slimeproducing ability of the isolates was determined with the Congo Red Agar (CRA) method (Freeman et al 1989).…”

Characterization of Tannase Protein Sequences of Bacteria and Fungi: An In Silico Study

“…Detannification of foods and feeds by applying tannase can improve their palatability, digestibility and nutritive value [1,19]. It is also used to treat tannin polluting industrial effluents and agricultural wastes [22,32]. Tannase is recently used in haze removing of tea beverages [33], tea cream solubilization [28] and as a biomarker for colon cancer [23].…”

Catalytic and Thermodynamic Properties of a Tannase Produced by Aspergillus niger GH1 Grown on Polyurethane Foam

“…But due to its hydrolytic and synthetic properties, tannase have several potential uses, such as the clarification of tanninrich beverages [4], the synthesis of antioxidants used in food industry [5,6], the elucidation of the structure of polyphenolic compounds [7], and the additive in laundry detergents [8]. Tannase could also be utilized for improvement of nutritional properties of legume flours and animal feed [9,10], bioremediation of tannin-contaminated wastewaters [11], and in the pre-treatment of lignocellulosic materials used for ethanol production [12].…”