ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.)

Abstract

Antibacterial Extract of Mangosteen (Garcinia mangostana L.)Fruit Shell The skin of the mangosteen fruit is extracted with n-hexane and ethyl acetate to determine the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27 853. Results showed that n-hexane extract gave inhibition zone larger than the ethyl acetate extract on all concentrations . Extract n-hexane has a value of MIC against S. aureus ATCC bacterial test 25923 62.5 mg / ml while the ethyl acetate extract of 125 mg / ml . N- hexane extracts had MIC values of the test bacteria P.aeuroginosa ATCC 27 853 was 125 mg / ml , and while the ethyl acetate extract had a MIC value of 500 mg / ml . Treatment of solvent, concentration and interaction between the solvent and concentration significantly affected the test bacteria ATCC 25923 S. aureus at the level of 5 %, the highest interaction N-Hexane solvent with a concentration of 15,625 mg / ml and was not significantly different interactions with the concentration of 31.25 mg/ml and 125 mg/ml. Treatment solvent and concentration significantly while the interaction between the solvent and the concentration has no effect on the test bacteria P.aeuroginosa ATCC 27 853 at 5% level .Keywords: Garcinia mangostana L., Staphylococcus aureus and Pseudomonas aeruginosa ABSTRAK Kulit buah manggis diekstrak dengan n-heksan dan etil asetat untuk mengetahui Konsentrasi Hambat Minimum (KHTM) terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 27853. Hasil penelitian menunjukkan bahwa ekstrak n-heksana memberikan zona hambatan lebih besar dibandingkan dengan ekstrak etil asetat pada semua konsentrasi. Ekstrak n-heksana memiliki nilai kadar hambat minimal (KHM) terhadap bakteri uji S. aureus ATCC 25923 62,5 mg/ml sedangkan ekstrak etil asetat 125 mg/ml. Ekstrak n-heksana memiliki nilai KHM terhadap bakteri uji P.aeuroginosa ATCC 27853 adalah 125 mg/ml dan sedangkan ekstrak etil asetat memiliki nilai KHM 500 mg/ml. Perlakuan pelarut, konsentrasi dan interaksi antara pelarut dan konsentrasi berpengaruh nyata terhadap bakteri uji S. aureus ATCC 25923 pada taraf 5%, interaksi tertinggi yaitu pelarut N-Heksan dengan konsentrasi 15,625mg/ml dan interaksi ini tidak berbeda nyata dengan konsentrasi 31,25 mg/ml dan 125 mg/ml. Perlakuan pelarut dan konsentrasi berpengaruh nyata sedangkan interaksi antara pelarut dan konsentrasi tidak berpengaruh terhadap bakteri uji P.aeuroginosa ATCC 27853 pada taraf 5%. Kata kunci: Garcinia mangostana L., Staphylococcus aureus dan Pseudomonas aeruginosa

Antibacterial Activity Test of Mangosteen Plants (Garcinia Mangostana L.): A Review

“…Based on the antibacterial effectiveness of some parts of the mangosteen plant, Mangosteen fruit peel has the highest antibacterial effect than any other part. Especially on the extraction separation method using a solvent n-hexane [18] . N-hexanes are non-polar solvents, so the non-polar compound group will dissolve in this solvent [18] .…”

“…Especially on the extraction separation method using a solvent n-hexane [18] . N-hexanes are non-polar solvents, so the non-polar compound group will dissolve in this solvent [18] . The nhexane solvent can attract flavonoids, tannins, and xanthones contained in the mangosteen fruit peel.…”

“…N-hexane extract of mangosteen peel contains antibacterial compounds, namely flavonoids, tannins, and xanthones that can inhibit the growth of Staphylococcus aureus bacteria [18] . Staphylococcus aureus bacteria are gram-positive bacteria that have a layer cell wall composed of peptidoglycan macromolecules and a single cell membrane composed of proteins and lipids [41] .…”