Antibacterial wound dressing nanofibrous mats composed of cellulose acetate loaded with chitosan/erythromycin nanoparticles

Abstract

This study attempts to fabricate the antibacterial nanofibrous mats composed of cellulose acetate (CA) nanofibers loaded with erythromycin-chitosan nanoparticles (Ery-CS NPs) intended for infected wound dressing. The Ery-loaded CS NPs were prepared by ionic gelation process and then incorporated into the CA electrospun nanofibers (NFs). The characterization of the NPs and mats were investigated by using dynamic light scattering (DLS), scanning electron microscopy (SEM), Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) to show the size distribution, morphology and surface chemistry along with other physiochemical properties including hydrophilicity property, porosity, and water absorption capacity. The antimicrobial activity and cell viability of fibroblast cells were also evaluated. The results indicated that Ery was loaded into CS NPs with high encapsulation efficiency (95%). The CA NFs (17%) incorporated with the Ery-CS NPs (12 wt%) displayed smooth homogenous morphology with 141.7 ± 91.7 nm average diameter. The relevant analyses revealed that the NPs incorporated in NFs and provided high water holding capacity with high porosity. Finally, Ery-CS NPs/CA mats were able to inhibit the growth of both Gram-positive and Gram-negative bacteria as well as showed no cytotoxic effect on the human dermal fibroblast cells. Overall, our findings concluded that the proposed system could be potentially applied as the proper antibacterial mats for infected wound dressing applications.

An Overview on the Recent Advances in the Treatment of Infected Wounds: Antibacterial Wound Dressings

“…[ 131,132 ] The usual dose of topical Ert is reported about 20 mg g −1 . [ 133 ] Ert is known as an antibacterial agent and is often used for gram‐positive bacterial infections. It first inhibits protein synthesis by binding to the 23S bacterial ribosomal RNA molecule in the 50S subunit.…”

“…The results showed that these prepared nanofibers have good antibacterial activity against gram‐positive and gram‐negative bacteria and on the other hand, no cytotoxicity was observed on fibroblast cells. [ 133 ]…”

Potential of Nanocarriers for Wound Healing

“…Doostanet alexplored the antibacterial potential of a medium spectrum antibiotic, erythromycin, for infected wound care management. A cellulose acetate nanofibrous mat incorporated with erythromycin loaded chitosan nanoparticles was evaluated against E. coli, S. aureusand P. aeruginosa bacterial strains as potential dressing for infected wounds [11]. A recent study reported an increase in poor solubility of curcumin via encapsulation into a saline-hydrogel nanoparticulatevector.…”

Functional nanoparticles in electrospun fibers for biomedical applications

“…For example, the hybrid nanofibers can be fabricated by directly blending the target NPs with the polymer solution for electrospinning, [ 29 ] sol‐gel emulsification method for wrapping the NPs within the fiber, [ 30 ] layer by layer stacking, [ 8 ] in situ growth or coating of NPs on the nanofibers surface. [ 31 ] The NPs in electrospun fibers hybrid composites display a broad application potency ranging from wound dressings with antimicrobial and/or angiogenesis bioactivities, [ 32 ] osteogenesis [ 33 ] and periosteal regeneration, [ 34 ] anti‐tumor treatment, [ 35 ] biosensing [ 36 ] and gene delivery [ 9 ] (Scheme 1).…”