Antibacterial susceptibility profiles of subclinical mastitis pathogens isolated in Batna and Setif Governorates East of Algeria

Mamache, Bakir; Sabrina, Rabehi; Meziane, T.

doi:10.5455/vetworld.2011.537-541

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…Among the 229 staphylococcal isolates from the 132 cows with SCM, the majority were CoNS, that is, 197 isolates from 121 cows, suggesting that CoNS are the predominant bacterial pathogens associated with SCM in Thailand. This finding is in agreement with the previous findings in Thailand ( Jarassaeng et al, 2012 ), Sweden ( Persson, Nyman & Andersson, 2011 ), eastern Algeria ( Bakir, Sabrina & Toufik, 2011 ), Dharwad, India ( Kaliwal et al, 2011 ), Northwest Iran ( Hosseinzadeh & Saei, 2014 ) and Jiangsu Province, China ( Xu et al, 2015 ). Although CoNS usually cause infections with less severe symptoms compared to the S. aureus infections, they are highly contagious and can be spread readily to other cattle in the herd, other herds, as well as other animals and humans ( Xu et al, 2015 ) through direct contact or via the contaminated environmental sources such as manure, bedding, vegetation, ground, forage, water.…”

Section: Discussionsupporting

confidence: 93%

Staphylococcusspp. associated with subclinical bovine mastitis in central and northeast provinces of Thailand

Pumipuntu

Tunyong

Chantratita

et al. 2019

PeerJ

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Background Staphylococcus spp. are major cause of bovine mastitis (BM) worldwide leading to economic damage to dairy farms and public health threat. Recently, a newly emerged Staphylococcus argenteus has been found as a human and animal pathogen. Molecular characteristics, virulence and antibiotic resistant phenotypes of bacteria causing BM in Thailand are rare. This study aimed to investigated Staphylococcus spp. associated with subclinical bovine mastitis (SCM) in Thailand. Methods Milk samples were collected from 224 cows of 52 dairy herds in four central and northeast provinces. Total somatic cell counts (SCC) and California mastitis test (CMT) were used to identify SCM cows. Milk samples were cultured for Staphylococcus spp. Coagulase-positive isolates were subjected to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Organisms suspected as S. argenteus were verified by detecting nonribosomal peptide synthetase gene. All isolates were checked for antibiograms and the presence of various virulence genes. Results From the 224 milk samples of 224 cows, 132 (59%) were positive for SCM by SCC and CMT and 229 staphylococcal isolates were recovered. They were 32 coagulase-positive (24 S. aureus and eight S. argenteus) and 197 coagulase-negative. PFGE of the S. aureus and S. argenteus revealed 11 clusters and a non-typeable pattern. MLST of representatives of the 11 PFGE clusters, three PFGE non-typeable S. aureus isolates from different locations and S. argenteus showed 12 sequence types. The eight S. argenteus isolates belonged to ST1223 (three isolates), ST2250 (two isolates), and ST2793 (two isolates). The antimicrobial tests identified 11 (46%) methicillin-resistant S. aureus and 25 (13%) methicillin-resistant coagulase-negative isolates, while seven S. argenteus were methicillin-susceptible and one isolate was methicillin-resistant. All of the 229 isolates were multiply resistant to other antibiotics. The most prevalent virulence genes of the 24 S. aureus isolates were clfA, coa and spa (X and IgG-binding region) (100%), hla (96%), pvl (96%) and sec (79%). Six S. argenteus isolates carried one enterotoxin gene each and other virulence genes including coa, clfA, hla/hlb, spa, tsst and pvl, indicating their pathogenic potential. Conclusion and perspective This is the first report on the S. argenteus from cow milk samples with SCM. Data on the molecular characteristics, virulence genes and antibiograms of the Staphylococcus spp. obtained from the present study showed a wide spread and increasing trend of methicillin-resistance and multiple resistance to other antibiotics. This suggests that the “One Health” practice should be nurtured, not only at the dairy farm level, but also at the national or even the international levels through cooperation of different sectors (dairy farmers, veterinarians, medical and public health personnel and scientists) in order to effectively combat and control the spread of these pathogens.

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Section: Discussionsupporting

confidence: 93%

Staphylococcusspp. associated with subclinical bovine mastitis in central and northeast provinces of Thailand

Pumipuntu

Tunyong

Chantratita

et al. 2019

PeerJ

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“…Usually intramammary infusion is the most commonly recommended route to treat mastitis cases and one of the antibiotics that has a good distribution into the mammary gland for mastitis treatment is spiramycin [35,36]. In many studies [23,25,37], strains isolated from subclinical mastitis including Streptococcus spp., Staphylococcus spp. and Enterococcus spp.…”

Section: Discussionmentioning

confidence: 99%

Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

et al. 2015

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The aim of this study was to investigate the etiology of subclinical mastitis (SCM) in dairy Jersey cows with the use of bacteriological and molecular identifi cation methods. In the study 121 Jersey and 78 hybrid Jersey cows with SCM were observed in the Samsun district of Turkey. A total of 411 California mastitis test (CMT) positive milk samples from these animals were examined bacteriologically. The prevalence of subclinical mastitis was 54.75% and 67.2% in Jerseys and hybrids, respectively. On bacteriological examination, a total of 92 strains were isolated from 411 milk samples. The most prevalent bacteria were Staphylococcus spp. (69.56%). Among them 24 isolates were Staphylococcus aureus (26.08%) the other isolates were Streptococcus dysgalactiae (23.91%), Enterococcus spp. (3.26%) and Streptococcus agalactiae (3.26%). All strains were identifi ed with bacteriological culture methods, as well as by Polymerase Chain Reaction (PCR). Gram-negative bacteria were not isolated. In conclusion, the etiology of SCM in full blood and hybrid Jersey dairy cows in Samsun and the prevalence of bacteria were determined. The relatively high prevalence of SCM indicates the potential need for the consideration of some factors contributing to the formation of mastitis (e.g. management) as well as bacterial agents. The present study and further studies may be useful to develop mastitis vaccines by means of providing true vaccine strain sources.

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“…This comparing data showed the high percentage of S. aureus infection in bovine mastitis from the past which needs to be concerned for both livestock and veterinary public health. An increase in the prevalence of S. aureus infections associated with bovine mastitis has also been reported in other countries; Northwest Iran [20], Jiangsu Province, China [2], Sweden [21], Eastern Algeria [22], and Dharwad, and India [23].…”

Section: Discussionmentioning

confidence: 91%

Staphylococcus argenteus: An emerging subclinical bovine mastitis pathogen in Thailand

Pumipuntu

2019

Vet World

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Background and Aim: Staphylococcus argenteus is an emerging species of the Staphylococcus aureus complex. It has usually been misidentified as S. aureus by conventional methods and its characteristics. S. argenteus is potentially emerging in both humans and animals with an increasing global distribution. This study aimed to differentiate and identify S. argenteus from S. aureus collected and isolated from milk samples of subclinical bovine mastitis cases in Maha Sarakham Province, Northeastern Thailand. Materials and Methods: Forty-two isolates of S. aureus were studied from 132 individual milk samples collected from subclinical bovine mastitis cases of 15 dairy farms in three districts of Maha Sarakham, Thailand. The identification was confirmed by conventional and immune-agglutination methods. Fifteen representative isolates which were suspected as being S. argenteus were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results: The result from MALDI-TOF MS confirmed that seven from 15 isolates were S. argenteus and eight isolates were S. aureus. Conclusion: This study indicated that MALDI-TOF MS used as an identification and classification method could accurately differentiate the novel species, S. argenteus, from the S. aureus complex which is usually misdiagnosed. In addition, the identification of S. argenteus seems to be very limited in technical difficulty despite the fact that it may be the important causative pathogen in bovine mastitis as well as a pathogenic bacterium in food and milk. Therefore, it is essential for both bovine medicine and veterinary public health to emphasize and recognize this bacterial pathogen as an emerging disease of staphylococcal bacteria that there is a need for further study of S. argenteus infections.

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Antibacterial susceptibility profiles of subclinical mastitis pathogens isolated in Batna and Setif Governorates East of Algeria

Cited by 7 publications

References 2 publications

Staphylococcusspp. associated with subclinical bovine mastitis in central and northeast provinces of Thailand

Staphylococcusspp. associated with subclinical bovine mastitis in central and northeast provinces of Thailand

Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

Staphylococcus argenteus: An emerging subclinical bovine mastitis pathogen in Thailand

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