Antibacterial properties of snake venom components

Bocian, Aleksandra; Hus, Konrad K.

doi:10.1007/s11696-019-00939-y

Cited by 23 publications

(22 citation statements)

References 123 publications

(122 reference statements)

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“…Therefore, it is difficult to speculate on the participation of this group in the bactericidal effect. As for the other groups, reports indicate their antibacterial character [14]. What is very interesting is that the composition of F2 fraction is quite similar to F1, although there is no LAAOs but there are SVMPs and, more importantly, cathelicidin-like antimicrobial peptides, which have a wide spectrum of antibacterial activity and high efficiency [15,16].…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis

et al. 2020

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One of the key problems of modern infectious disease medicine is the growing number of drug-resistant and multi-drug-resistant bacterial strains. For this reason, many studies are devoted to the search for highly active antimicrobial substances that could be used in therapy against bacterial infections. As it turns out, snake venoms are a rich source of proteins that exert a strong antibacterial effect, and therefore they have become an interesting research material. We analyzed Naja ashei venom for such antibacterial properties, and we found that a specific composition of proteins can act to eliminate individual bacterial cells, as well as the entire biofilm of Staphylococcus epidermidis. In general, we used ion exchange chromatography (IEX) to obtain 10 protein fractions with different levels of complexity, which were then tested against certified and clinical strains of S. epidermidis. One of the fractions (F2) showed exceptional antimicrobial effects both alone and in combination with antibiotics. The protein composition of the obtained fractions was determined using mass spectrometry techniques, indicating a high proportion of phospholipases A2, three-finger toxins, and L-amino acids oxidases in F2 fraction, which are most likely responsible for the unique properties of this fraction. Moreover, we were able to identify a new group of low abundant proteins containing the Ig-like domain that have not been previously described in snake venoms.

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Section: Discussionmentioning

confidence: 97%

Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis

et al. 2020

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“…Moreover, selected components of snake venoms may be also considered as new pharmaceuticals with high therapeutical potential such as FDA approved drugs based on snake venoms Captopril ® (Enalapril, an inhibitor of angiotensin-converting enzyme), Integrilin ® (Eptifibatide, glycoprotein IIb/IIIa inhibitor) and Aggrastat ® (Tirofiban, glycoprotein IIb/IIIa inhibitor) to treat hypertension, acute coronary syndrome and heart attack, respectively [8,9]. In contrast to well established antibacterial activity of crude snake venoms and selected components such as PLA 2 and LAAO fractions [10][11][12], little is known about antifungal properties of snake venoms, especially elapid snake venoms (Elapidae family) [9]. Thus, it seems reasonable to study antifungal activity of cobra venoms more comprehensively.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

Kuna

Bocian

Hus

et al. 2020

Toxins

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In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three Candida species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of C. albicans and C. tropicalis, affected cell cycle of C. albicans, inhibited C. tropicalis-based biofilm formation and promoted oxidative stress in C. albicans, C. glabrata and C. tropicalis cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three Candida species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of Candida species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate Candida pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments.

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“…In this respect, the efficiency of these compounds, specially phospholipase A2 against antibioticresistant bacteria, holds promise for biotechnological applications, in this case, new medical treatment alternatives, however, it should be understood there are different antibacterial activity mechanisms from venom-based drugs [23,24]. Antibacterial activity of rattlesnake's venoms against P. aeruginosa: (a) columns 1-3, CT1 from 100 at 0.78 µg/mL, columns 4-6, CR4 from 100 at 0.78 µg/mL, columns 7-9, CT2 from 100 at 0.78 µg/mL, columns 10-12, CR3 from 100 at 0.78 µg/mL.…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…In this respect, the efficiency of these compounds, specially phospholipase A 2 against antibiotic-resistant bacteria, holds promise for biotechnological applications, in this case, new medical treatment alternatives, however, it should be understood there are different antibacterial activity mechanisms from venom-based drugs [23,24].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Zaragoza-Bastida

Flores-Aguilar

Aguilar-Castro

et al. 2020

Animals

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Simple Summary: Rattlesnakes (Crotalus ravus and Crotalus triseriatus) have some compounds that resemble polypeptides and proteins in their venoms which can be used in therapeutic treatment as antibacterial compounds. The aim of the present study is to evaluate the antibacterial and hemolytic activity of two rattlesnake venoms. The results of the present study indicate that the evaluated venoms have bactericidal activity against Pseudomonas aeruginosa, an important bacterium that affects animals and humans, thereby providing a new and efficient treatment alternative against this pathogenic bacterium.Abstract: Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

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Antibacterial properties of snake venom components

Cited by 23 publications

References 123 publications

Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis

Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis

Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

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